FlyBase Allele Report: Dmel\tws[S061805]

General Information

Symbol

Dmel\tws^S061805

Species

D. melanogaster

Name

FlyBase ID

FBal0100058

Feature type

allele

Associated gene

Dmel\tws

Associated Insertion(s)

P{lacW}tws^S061805

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Deak et al., 1997)

P-element activity

(Szeged Stock Center, 1998-)

Progenitor genotype

Associated Insertion(s)

P{lacW}tws^S061805

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with Df(3R)by62)

(Deak et al., 2003, Szeged Stock Center, 1998-, Deak et al., 1997)

lethal - all die before end of P-stage | recessive

(Deak et al., 1997)

partially lethal - majority die | recessive

(Szeged Stock Center, 1998-)

some die during pharate adult stage | recessive

(Deak et al., 1997)

Phenotype Manifest In

mitotic anaphase & neuroblast

(Deak et al., 2003)

mitotic metaphase & neuroblast

(Deak et al., 2003)

spindle & neuroblast

(Deak et al., 2003)

Detailed Description

Statement

Reference

Anaphase is abnormal in tws^S061805 mutant cells.

(Deak et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

tws^S061805 has abnormal mitotic cell cycle phenotype, suppressible by Cdc27^S092309

(Deak et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

tws^S061805 has mitotic anaphase phenotype, suppressible by Cdc27^S092309

(Deak et al., 2003)

tws^S061805 has mitotic telophase phenotype, suppressible by Cdc27^S092309

(Deak et al., 2003)

Enhancer of

Statement

Reference

tws^S061805 is an enhancer of mitotic anaphase phenotype of Cdc27^S092309

(Deak et al., 2003)

tws^S061805 is an enhancer of mitotic telophase phenotype of Cdc27^S092309

(Deak et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

There is no apparent shift in the lethal phase in the Cdc27^S092309 tws double mutant. Cytological analysis of neuroblasts show an elevated mitotic index and a dramatic reduction of metaphase figures replaced by an elevated frequency of anaphase-telophase figures equivalent to the tws^S061805 mutant alone. Therefore the Cdc27^S092309 tws^S061805 double mutant overcomes the metaphase arrest seen in the Cdc27^S092309 single mutant. From the analysis of CycB localisation, the authors postulate that Cdc27^S092309 tws^S061805 double mutant mitotic cells are capable of entering anaphase without activation of the APC/C. However, anaphase in these cells is highly abnormal.

(Deak et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

0618/05

(Deak et al., 1997)

l(3)S061805^S061805

tws^618/5

(Deak et al., 2003)

tws^S061805

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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