FB2024_03 , released June 25, 2024
Allele: Dmel\Lis-1D
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General Information
Symbol
Dmel\Lis-1D
Species
D. melanogaster
Name
FlyBase ID
FBal0101617
Feature type
allele
Associated gene
Dmel\Lis-1
Associated Insertion(s)
Carried in Construct
Also Known As
Lis1D
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Steward, 2000.8.14, Liu et al., 1999)
Progenitor genotype
Cytology
Description

Amino acid replacement: W324term.

(Liu et al., 1999)

Nucleotide substitution: G1160A.

(Liu et al., 1999)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:16,183,346..16,183,346 [+]
Nucleotide change:

G16183346A

Reported nucleotide change:

G1160A

Amino acid change:

W324term | Lis-1-PA; W324term | Lis-1-PB; W324term | Lis-1-PF; W324term | Lis-1-PG

Reported amino acid change:

W324term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Liu et al., 1999)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Only 2% of Ecol\lacZarm.PV-negative germline stem cells remain three weeks after Lis-1D clone induction, compared to 75-80% in wild-type clones. Apoptotic Lis-1D mutant germline stem cells are not found, indicating that Lis-1 is required for controlling germline stem cell self-renewal but not survival in the ovary.

      Lis-1D mutant germline stem cells produce fewer germline cysts than control germline stem cells. The percentage of BrdU-positive Lis-1D mutant germline stem cells is similar or even higher than that of control germline stem cells. Lis-1D mutant germline stem cells exhibit a misoriented spindle more frequently than control germline stem cells.

      Lis-1D heterozygous germaria have about one cystoblast, as in wild-type germaria.

      (Chen et al., 2010)

      Females containing homozygous germ line clones fail to produce any eggs. No vitellogenic oocytes are seen in the ovaries.

      (Liu et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      NOT suppressed by
      Suppressor of
      Statement
      Reference

      Lis-1[+]/Lis-1D is a suppressor of germarium | ectopic | germline clone phenotype of Scer\GAL4VP16.nanos.UTR, tkvca.UASp

      (Chen et al., 2010)

      Lis-1[+]/Lis-1D is a suppressor of cystoblast | ectopic | germline clone phenotype of Scer\GAL4VP16.nanos.UTR, tkvca.UASp

      (Chen et al., 2010)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Lis-1D heterozygous germaria overexpressing tkvca.Scer\UAS.P\T under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 exhibit significantly fewer cystoblasts than wild-type germaria overexpressing tkvca.Scer\UAS.P\T.

      Expression of constitutively active tkvQ199D.Scer\UAS.P\T in Lis-1D heterozygous germaria under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 slows the loss of two week old Lis-1D mutant germline stem cells dramatically. This rescue effect becomes less dramatic for 3 week old mutant germline stem cells.

      Germline specific expression of shgScer\UAS.P\T.cCa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 slows the loss of 2 week old Lis-1D mutant germline stem cells dramatically and the rescue effect is also decreased for the 3 week old mutant germline stem cells.

      (Chen et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Lis-1D is rescued by Lis-1+t16

      (Liu et al., 1999)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Lis-1D
      Lis1D
      (Chen et al., 2010, Steward, 2000.8.14, Liu et al., 1999)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)