FB2024_03 , released June 25, 2024
Allele: Dmel\tkvQ199D.UASp
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General Information
Symbol
Dmel\tkvQ199D.UASp
Species
D. melanogaster
Name
FlyBase ID
FBal0241489
Feature type
allele
Associated gene
Dmel\tkv
Associated Insertion(s)
Carried in Construct
P{UASp-tkv.Q199D}
Also Known As
UASp-tkv*
Key Links
Transgenic product class
missense_variant
(Bolivar et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Bolivar et al., 2006)
Mutagen
in vitro construct
(Fichelson et al., 2009, Bolivar et al., 2006)
Progenitor genotype
Carried in construct
P{UASp-tkv.Q199D}
Cytology
Description

UASp regulatory sequences drive expression of an activated form of tkv which carries the Q199D amino acid substitution.

(Bolivar et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
tkv
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Germaria are filled with single cells dividing away from the niche in random orientations in females expressing tkvact.Scer\UAS.P\T under the control of Scer\GAL4nos.UTR.T:Hsim\VP16.

      (Fichelson et al., 2009)

      Expression of tkvQ199D.Scer\UAS.P\T under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 results in the overproliferation of germline stem cell-like cells in the ovary.

      Females in which expression of tkvQ199D.Scer\UAS.P\T under the control of Scer\GAL4bab1-Gal4 has been restricted to the adult stage using Scer\GAL80ts.αTub84B (animals are shifted to the restrictive temperature of 31[o]C when adult) do not show a germline stem cell tumour phenotype in the ovary after 3 days of tkvQ199D.Scer\UAS.P\T expression.

      (Bolivar et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of constitutively active tkvQ199D.Scer\UAS.P\T in Lis-1D heterozygous germaria under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 slows the loss of two week old Lis-1D mutant germline stem cells dramatically. This rescue effect becomes less dramatic for 3 week old mutant germline stem cells.

      Expression of constitutively active tkvQ199D.Scer\UAS.P\T in Lis-1G10.14 heterozygous germaria under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 slows the loss of two week old Lis-1G10.14 mutant germline stem cells dramatically. This rescue effect becomes less dramatic for 3 week old mutant germline stem cells.

      Expression of constitutively active tkvQ199D.Scer\UAS.P\T in Lis-1D heterozygous germaria under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 slows the loss of two week old Lis-1D mutant germline stem cells dramatically. This rescue effect becomes less dramatic for 3 week old mutant germline stem cells.

      (Chen et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      tkvQ199D.Scer\UAS.P\T
      tkvQ199D.UASp
      tkvact.Scer\UAS.P\T
      Name Synonyms
      Secondary FlyBase IDs
        References (4)