FlyBase Allele Report: Dmel\Raf[F20.UAS]

General Information

Symbol

Dmel\Raf^F20.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0103603

Feature type

allele

Associated gene

Dmel\Raf

Associated Insertion(s)

Carried in Construct

P{UAS-Raf.F20}

Also Known As

UAS-Δ-Raf^F20

Key Links

Transgenic product class

inframe_deletion

(Martin-Blanco et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Martin-Blanco et al., 1999)

Mutagen

in vitro construct

(Martin-Blanco et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-Raf.F20}

Cytology

Description

UASt regulatory sequences drive expression of a truncated form of phl, which lacks amino acids 2 to 431. Produces a constitutively active protein.

(Martin-Blanco et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Raf

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size | pupal stage, with Scer\GAL4^amn-c651

(Caldwell et al., 2005)

partially lethal, with Scer\GAL4⁶⁰⁴

(Martin-Blanco et al., 1999)

partially lethal - majority live, with Scer\GAL4⁶⁰⁴

(Morey et al., 2001)

visible, with Scer\GAL4⁶⁰⁴

(Martin-Blanco et al., 1999)

visible, with Scer\GAL4^GMR.PF

(Morey et al., 2001)

Phenotype Manifest In

bract | ectopic, with Scer\GAL4^hs.PB

(del Alamo et al., 2002)

bract | ectopic & adult external prothorax, with Scer\GAL4^hs.PB

(del Alamo et al., 2002)

bract | ectopic & arista, with Scer\GAL4^hs.PB

(del Alamo et al., 2002)

bract | ectopic & costal vein, with Scer\GAL4^hs.PB

(del Alamo et al., 2002)

bract | ectopic & gena, with Scer\GAL4^hs.PB

(del Alamo et al., 2002)

bract | ectopic & haltere, with Scer\GAL4^hs.PB

(del Alamo et al., 2002)

bract | ectopic & mesothoracic tergum, with Scer\GAL4^hs.PB

(del Alamo et al., 2002)

bract | ectopic & wing | dorsal, with Scer\GAL4^hs.PB

(del Alamo et al., 2002)

bract | ectopic & wing hinge | dorsal, with Scer\GAL4^hs.PB

(del Alamo et al., 2002)

eye, with Scer\GAL4^GMR.PF

(Morey et al., 2001)

femur, with Scer\GAL4^hs.PB

(del Alamo et al., 2002)

photoreceptor neuron, with Scer\GAL4^GMR.PF

(Morey et al., 2001)

trichome, with Scer\GAL4^hs.PB

(del Alamo et al., 2002)

wing, with Scer\GAL4⁶⁰⁴

(Morey et al., 2001, Martin-Blanco et al., 1999)

wing sensillum | ectopic, with Scer\GAL4⁶⁰⁴

(Martin-Blanco et al., 1999)

wing vein, with Scer\GAL4⁶⁰⁴

(Martin-Blanco et al., 1999)

wing vein | ectopic, with Scer\GAL4⁶⁰⁴

(Martin-Blanco et al., 1999)

wing vein L3, with Scer\GAL4⁶⁰⁴

(Morey et al., 2001)

wing vein L5, with Scer\GAL4⁶⁰⁴

(Morey et al., 2001)

Detailed Description

Statement

Reference

Expression of phl^F20.Scer\UAS under the control of Scer\GAL4^amn-c651 reduces pupal body size. This genotype has no effect on prothoracic gland cell size.

(Caldwell et al., 2005)

When phl^F20.Scer\UAS is driven by Scer\GAL4^hs.PB and heatshocked for 30 minutes at 37^oC, large territories of trichomes are transformed into bracts; in legs, the proximal femur to the claw, all trichomes are transformed into bracts. In the notum, the region between the dorsocentral bristles also differentiate multiple bracts. In wings, only the cells of the dorsal and ventral proximal costa differentiate as bracts; the rest of the wing cells are not affected, apart from a small area in the dorsal hinge that also that also differentiates a patch of bracts. In the halteres multiple bracts cover the dorsal and ventral anterior pedicelem. The prothorax also differentiates a small patch of bracts. In the head bracts are found at the base of the arista, and in the gena and the supraorbital region. The size and shape of bracts was constant in different parts of the fly. When phl^F20.Scer\UAS, Scer\GAL4^hs.PB flies are heatshock induced at 8 hours APF differentiate a large number of bracts, with almost all the trichomes from the above-defined competent areas transformed. Heat sock at later stages yield a reduced density of bracts, and earlier heat shock produces a pattern of regions of normal trichomes surrounding patches of bracts are mainly areas among bristles.

(del Alamo et al., 2002)

Expression of phl^F20.Scer\UAS (driven by Scer\GAL4^GMR.PF) leads to the recruitment of supernumerary R7 photoreceptor cells. When driven by Scer\GAL4⁶⁰⁴, flies also have reduced viability, and wing phenotypes when raised at 25^oC. The central region of wing vein L3 is deleted, there is a partial loss of vein L5 and an overall reduction in wing size.

(Morey et al., 2001)

Flies expressing phl^F20.Scer\UAS under the control of Scer\GAL4⁶⁰⁴ have reduced viability and show wing phenotypes; they have ectopic veins and sensilla and a loss of endogenous veins. The wing may also be reduced in size.

(Martin-Blanco et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Raf^F20.UAS, Scer\GAL4⁶⁰⁴ has wing vein L3 phenotype, suppressible by Sps1^k11320/SelD[+]

(Morey et al., 2001)

Raf^F20.UAS, Scer\GAL4⁶⁰⁴ has wing vein L5 phenotype, suppressible by Sps1^k11320/SelD[+]

(Morey et al., 2001)

Raf^F20.UAS, Scer\GAL4⁶⁰⁴ has wing phenotype, suppressible by Sps1^k11320/SelD[+]

(Morey et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

When animals containing Poxn^hs.sev and phl^F20.Scer\UAS (driven by Scer\GAL4^hs.PB) and heatshocked, an additive phenotype is seen, mechanosensory bristles are transformed into chemosensory bristles and all epidermal cells are transformed into bracts. A similar effect is seen by the combination of Poxn^hs.sev and Poxn^hs.sev (driven by Scer\GAL4^hs.PB).

(del Alamo et al., 2002)

The addition of SelD^k11320/+ to phl^F20.Scer\UAS, Scer\GAL4⁶⁰⁴ flies leads to almost wild-type vein pattern, although wing size is still reduced.

(Morey et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Raf^F20.Scer\UAS

Raf^F20.UAS

Raf^F20

(Caldwell et al., 2005)

phl^F20.Scer\UAS

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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