Expression of Csp2.Scer\UAS under the control of Scer\GAL4elav-C155 in a wild-type background has no significant effect on evoked excitatory junction potential amplitude at the neuromuscular junction in larvae. Features of spontaneous release are also normal in these larvae. Expression of Csp2.Scer\UAS under the control of Scer\GAL4elav-C155 or Scer\GAL4hs.PB results in temperature-sensitive lethality, with the animals dying during the third larval instar stage at 29oC. The lethality caused by expression of Csp2.Scer\UAS under the control of Scer\GAL4hs.PB is more severe in male than in female flies. Adult escapers carrying Csp2.Scer\UAS expressed under the control of Scer\GAL4elav-C155 have a shorter life span than normal at 25oC. The flies are extremely sluggish, show severely uncoordinated movements, and slip frequently. Body size is severely reduced and wing inflation is impaired, so that the wings appear severely crumpled. The regular ommatidial pattern is disrupted so that the eye appears rough. The eye is slightly elliptical in shape and some bristles are lost. Fusion of several ommatidial units is occasionally seen.
Csp2.UAS, Scer\GAL4elav-C155 has visible phenotype, suppressible | partially by Syx1Ahs.PW
Scer\GAL4elav-C155/Csp2.UAS is a non-suppressor of abnormal neurophysiology | third instar larval stage phenotype of Hip142/Hip141
Scer\GAL4elav-C155/Csp2.UAS is a non-suppressor of abnormal neurophysiology | third instar larval stage phenotype of Hip142/Df(3L)brm11
Csp2.UAS, Scer\GAL4elav-C155 has wing phenotype, suppressible | partially by Syx1Ahs.PW
Csp2.UAS, Scer\GAL4elav-C155 has eye phenotype, suppressible | partially by Syx1Ahs.PW
Scer\GAL4elav-C155/Csp2.UAS is a suppressor of neuromuscular junction | larval stage phenotype of Syx1Ahs.PW
Scer\GAL4elav-C155/Csp2.UAS is a non-suppressor of embryonic/larval neuromuscular junction phenotype of Hip142/Hip141
Scer\GAL4elav-C155/Csp2.UAS is a non-suppressor of embryonic/larval neuromuscular junction phenotype of Hip142/Df(3L)brm11
Expression of Csp2.Scer\UAS under the control of Scer\GAL4elav-C155 does not rescue the reduced amplitude of the excitatory junctional potential at the Hip142/Df(3L)brm11 neuromuscular junction at 23oC.
Expression of Csp2.Scer\UAS under the control of Scer\GAL4elav-C155 does not rescue the reduced amplitude of the excitatory junctional potential at the Hip141/Hip142 neuromuscular junction at 30oC.
The crumpled wing and rough eye phenotype of adult escapers carrying Csp2.Scer\UAS expressed under the control of Scer\GAL4elav-C155 is partially suppressed by Syx1Ahs.PW at 25oC and completely suppressed by daily heat shocks during late larval and pupal development in these flies (which increase expression of Syx1Ahs.PW). The mean evoked excitatory junction potential (EJP) amplitude at the neuromuscular junction is reduced by about 34% compared to wild type in larvae expressing Syx1Ahs.PW at 25oC. The EJP amplitude is normal if the larvae are also expressing Csp2.Scer\UAS under the control of Scer\GAL4elav-C155.
Scer\GAL4elav-C155/Csp2.UAS partially rescues CspR1
Expression of Csp2.Scer\UAS under the control of Scer\GAL4elav-C155 has no effect on spontaneous miniature excitatory junction potentials at the neuromuscular junction of CspR1 third instar larvae, but does rescue the evoked excitatory junction potential at the neuromuscular junction.