FlyBase Allele Report: Dmel\vvl[UAS.cCa]

General Information

Symbol

Dmel\vvl^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Certel

FlyBase ID

FBal0118024

Feature type

allele

Associated gene

Dmel\vvl

Associated Insertion(s)

Carried in Construct

P{UAS-vvl.C}

Also Known As

UAS-dfr

Key Links

Transgenic product class

cDNA

(Certel et al., 2000)

wild_type

(Certel et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Certel et al., 2000)

wild_type

(Certel et al., 2000)

Mutagen

in vitro construct

(Certel et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-vvl.C}

Cytology

Description

UASt regulatory sequences drive expression of a full length vvl cDNA.

(Certel et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

vvl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^Lim3.B

(Certel and Thor, 2004)

abnormal neurophysiology, with Scer\GAL4^GH146

(Komiyama et al., 2003)

abnormal size | adult stage, with Scer\GAL4^c564

(Junell et al., 2010)

lethal, with Scer\GAL4^69B

(Junell et al., 2010)

lethal, with Scer\GAL4^da.G32

(Junell et al., 2010)

lethal - all die before end of second instar larval stage, with Scer\GAL4^Cg.PA

(Junell et al., 2010)

lethal - all die before end of second instar larval stage, with Scer\GAL4^Hml.PG

(Junell et al., 2010)

majority die during larval stage, with Scer\GAL4^Lsp2.PH

(Junell et al., 2010)

melanotic mass phenotype | larval stage, with Scer\GAL4^Cg.PA

(Junell et al., 2010)

melanotic mass phenotype | larval stage, with Scer\GAL4^Hml.PG

(Junell et al., 2010)

neoplasia | adult stage, with Scer\GAL4^Ser.PGF

(Certel et al., 2000)

short lived, with Scer\GAL4^c564

(Junell et al., 2010)

viable, with Scer\GAL4^hs.PB

(Junell et al., 2010)

visible, with Scer\GAL4^Ser.PGF

(Certel et al., 2000)

Phenotype Manifest In

antennal glomerulus & neuron, with Scer\GAL4^GH146

(Komiyama et al., 2003)

larval transverse nerve | embryonic stage 16, with Scer\GAL4^Lim3.B

(Certel and Thor, 2004)

olfactory sensory organ & neuron & dendrite, with Scer\GAL4^GH146

(Komiyama et al., 2003)

wing | distal, with Scer\GAL4^Ser.PGF

(Certel et al., 2000)

wing cell | ectopic, with Scer\GAL4^Ser.PGF

(Certel et al., 2000)

Detailed Description

Statement

Reference

Expressing vvl^UAS.cCa under the control of Scer\GAL4^c564 results in severe organ malformation or absence in newly eclosed adults.

(Junell et al., 2010)

In vvl^Scer\UAS.cCa; Scer\GAL4^Lim3.B embryos, transverse nerve axons leave the ventral nerve cord normally, but are mistargeted to the ISNb muscle field - either sending collaterals into ISNb muscle targets or, in some cases, innervating these muscles.

(Certel and Thor, 2004)

When vvl^Scer\UAS.cCa is driven in the anterodorsal projection neurons (adPNs), the innervation of dorsal landmark glomeruli is largely unaffected, and loss of dendritic projections to these glomeruli are rarely observed. However, these adPNs also mistarget their dendrites to 3 out of 10 lateral landmark glomeruli, DA1 DA2 and VA4. Single cell clones expressing vvl^Scer\UAS.cCa do not exhibit a detectable phenotype.

(Komiyama et al., 2003)

Flies expressing vvl^Scer\UAS.cCa under the control of Scer\GAL4^Ser.PGF at 25^oC produce wings with occasional blistering within the region of Scer\GAL4^Ser.PGF expression at the distal end of the wing. Ectopic growth of intervein tissue is consistently observed resulting in a small outgrowth at the most distal wing tip. The outgrowths do not appear to be organised with the characteristics of a partial wing duplication but seem to result from aberrant cell proliferation at the distal tip of the wing.

(Certel et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Lim3.B, vvl^UAS.cCa has abnormal neuroanatomy phenotype, suppressible by tup^unspecified

(Certel and Thor, 2004)

Enhancer of

Statement

Reference

vvl^UAS.cCa/Scer\GAL4^GH146 is an enhancer of abnormal neurophysiology | somatic clone phenotype of acj6⁶

(Komiyama et al., 2003)

Other

Statement

Reference

Avic\GFP^S65T.CecA1, Scer\GAL4^hs.PB, vvl^UAS.cCa has lethal phenotype

(Junell et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Lim3.B, vvl^UAS.cCa has larval transverse nerve | embryonic stage 16 phenotype, suppressible by tup^unspecified

(Certel and Thor, 2004)

Enhancer of

Statement

Reference

vvl^UAS.cCa/Scer\GAL4^GH146 is an enhancer of antennal lobe glomerulus DL1 | somatic clone phenotype of acj6⁶

(Komiyama and Luo, 2007)

vvl^UAS.cCa/Scer\GAL4^GH146 is an enhancer of adult antennal lobe projection neuron DL1 adPN | somatic clone phenotype of acj6⁶

(Komiyama and Luo, 2007)

vvl^UAS.cCa/Scer\GAL4^GH146 is an enhancer of antennal glomerulus DL1 & neuron | somatic clone phenotype of acj6⁶

(Komiyama et al., 2003)

Suppressor of

Statement

Reference

vvl^UAS.cCa/Scer\GAL4^lab.PH is a suppressor of larval tritocerebrum phenotype of lab¹⁴

(Meier et al., 2006)

NOT Suppressor of

Statement

Reference

vvl^UAS.cCa/Scer\GAL4^lab.PH is a non-suppressor of embryonic/larval tritocerebral commissure phenotype of lab¹⁴

(Meier et al., 2006)

vvl^UAS.cCa/Scer\GAL4^lab.PH is a non-suppressor of frontal connective phenotype of lab¹⁴

(Meier et al., 2006)

Other

Statement

Reference

Scer\GAL4^GH146, acj6⁶, ct^UAS.cPa, vvl^UAS.cCa has antennal lobe glomerulus DM2 | somatic clone phenotype

(Komiyama and Luo, 2007)

Scer\GAL4^GH146, acj6⁶, ct^UAS.cPa, vvl^UAS.cCa has antennal lobe glomerulus DA2 | somatic clone phenotype

(Komiyama and Luo, 2007)

Scer\GAL4^GH146, acj6⁶, vvl^UAS.cCa has antennal glomerulus DL1 & neuron | somatic clone phenotype

(Komiyama et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

vvl^Scer\UAS.cCa; Scer\GAL4^lab.PH partially suppresses the brain phenotype of lab¹⁴ homozygous embryos: longitudinal axon pathways in the tritocerebrum are restored but the tritocerebral commissure remains absent and axonal projection of the frontal connective remain abnormal.

(Meier et al., 2006)

The addition of vvl^Scer\UAS.cCa (driven by Scer\GAL4^GH146) to acj6⁶ single cell clones in DL1 leads to a complete failure to innervate the glomerulus DL1. Most of their dendrites partially innervated one or two glomeruli anterior to DL1, with some wandering dendrites within the dorsal half of the antennal lobe. This combination of mutants also leads to a novel stereotyped phenotype in DL1 projection neurons. The dorsal branch has additional side branches; the lateral branch stops well short of the lateral edge of the lateral horn and instead curves and branches to innervate the ventral area of the lateral horn nerver innervated by wild-type DL1 axons.

(Komiyama et al., 2003)

Xenogenetic Interactions

Statement

Reference

Expressing vvl^UAS.cCa under the control of Scer\GAL4^hs.PB together with Avic\GFP^S65T.CecA1 is lethal during development, but females can reach adulthood under lower expression conditions (18[o]C).

(Junell et al., 2010)

Complementation and Rescue Data

Partially rescues

vvl^UAS.cCa/Scer\GAL4^NP2646 partially rescues vvl^dfr-E82

(Hasegawa et al., 2011)

Comments

Expression of vvl^Scer\UAS.cCa under the control of Scer\GAL4^{CG11873-NP2646} partially rescues the Tm27 neuron phenotype of homozygous vvl^dfr-E82 clones.

(Hasegawa et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

vvl^Scer\UAS.cCa

vvl^UAS.cCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Certel

(Certel et al., 2000)

Secondary FlyBase IDs

References (7)

Report Sections

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