FlyBase Allele Report: Scer\GAL4[cry.PE]

General Information

Symbol

Scer\GAL4^cry.PE

Species

S. cerevisiae

Name

cryptochrome promoter construct of Emery

FlyBase ID

FBal0118345

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{cry-GAL4.E}

Also Known As

cry-GAL4, crypiGAL4, cryGal4, cry13-GAL4, cry-gal4(39), cry-gal4¹⁹, cry-GAL4-39

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Emery et al., 2000)

Progenitor genotype

Carried in construct

P{cry-GAL4.E}

Cytology

Description

The whole upstream region with the first intron and part of the second exon is fused in frame with sequence encoding a GAL4 driver.

(Emery et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

cry

cry_5.5kb

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

pacemaker neuron

(Daniels et al., 2008)

adult s-LNv neuron

(Daniels et al., 2008)

larval DN1 neuron

(Daniels et al., 2008)

Additional Information

Statement

Reference

Drives expression in a subset of pacemaker neurons.

(FlyBase Curators, 2017)

ScerGAL4^cry.PE is expressed in all of the LNv, LNd, DN3 and DN1 neurons in the protocerebrum. It also labels the medulla projections of LNv and DN3 and a lamina projection from the fifth s-LNv PDF-negative neuron. The staining intensity in the cell bodies in the accessory medulla was stronger in the l-LNv than in the s-LNv cells. There is also staining in restricted domains in the lobula which did not originated from the neurons that projected to the medulla.

(Damulewicz and Pyza, 2011)

Expression is within all LN neurons, a subset of the DN1 and DN3 neurons, and not at all in the DN2 neurons. Expression was not seen in the third instar larval Pdf negative s-LNv neuron. Fibres of the Pdf negative s-LNV neuron were observed to project towards the accessory medulla and arborize in its central area.

(Helfrich-Forster et al., 2007)

Marker for

Reflects expression of

cry

(Damulewicz and Pyza, 2011, Yoshii et al., 2008, Helfrich-Forster et al., 2007)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage, with Hsap\PRODH^UAS.cZa

(Zwarts et al., 2017)

abnormal circadian behavior, with hid^UAS.cZa

(Kaneko et al., 2012, Yoshii et al., 2010)

abnormal circadian rhythm, with Clk^UAS.cZa

(Zhao et al., 2003)

abnormal circadian rhythm, with mir-210^UAS.cCa

(Cusumano et al., 2018)

abnormal circadian rhythm, with Shaw^{RA.UAS.Tag:FLAG}

(Hodge and Stanewsky, 2008)

abnormal circadian rhythm | adult stage, with Grd^GD2702, Scer\GAL80^Pdf.PS

(Frenkel et al., 2017)

abnormal circadian rhythm | adult stage, with Scer\GAL80^Pdf.PS, SecCl^JF02437

(Frenkel et al., 2017)

abnormal locomotor rhythm

(Shafer et al., 2008)

abnormal locomotor rhythm, with Clk^UAS.cZa

(Zhao et al., 2003)

abnormal locomotor rhythm, with Cul3^NIG.11861R

(Grima et al., 2012)

abnormal locomotor rhythm, with Fer2LCH^KK103577

(Mandilaras and Missirlis, 2012)

abnormal locomotor rhythm, with hid^UAS.cZa

(Kaneko et al., 2012, Stoleru et al., 2004)

abnormal locomotor rhythm, with mir-210^UAS.cCa

(Cusumano et al., 2018)

abnormal locomotor rhythm, with Scer\GAL80^Pdf.PS, hid^UAS.cZa

(Stoleru et al., 2004)

abnormal locomotor rhythm, with Shaw^{RA.UAS.Tag:FLAG}

(Hodge and Stanewsky, 2008)

abnormal locomotor rhythm | adult stage, with me31B^NIG.4916R

(Lee et al., 2017)

abnormal sleep, with Bhal\NaChBac^UAS.EGFP, Scer\GAL80^Pdf.PS

(Shang et al., 2008)

abnormal sleep, with Hsap\KCNJ2^UAS.EGFP, Scer\GAL80^Pdf.PS

(Shang et al., 2008)

viable, with Clk^UAS.cZa

(Zhao et al., 2003)

viable, with Cul3^NIG.11861R

(Grima et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

A Scer\GAL4^cry.PE/+ background causes high levels of arrhythmicity under free-running conditions.

(Shafer et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^cry.PE, me31B^NIG.4916R has abnormal locomotor rhythm | adult stage phenotype, enhanceable by Atx2^KK100423, Scer\GAL4^cry.PE

(Lee et al., 2017)

Enhancer of

Statement

Reference

Atx2^KK100423, Scer\GAL4^cry.PE is an enhancer of abnormal locomotor rhythm | adult stage phenotype of Scer\GAL4^cry.PE, me31B^NIG.4916R

(Lee et al., 2017)

per^UAS.cBa, per⁰¹, Scer\GAL4^cry.PE, per[+] is an enhancer of abnormal circadian rhythm phenotype of cwo^PB9

(Richier et al., 2008)

Suppressor of

Statement

Reference

per^UAS.cBa/Scer\GAL4^cry.PE is a suppressor | partially of abnormal locomotor rhythm | adult stage phenotype of Lsm12a^Δ6

(Lee et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^cry.PE/Atx2^UAS.Tag:FLAG is a non-suppressor of abnormal locomotor rhythm | adult stage phenotype of Lsm12a^Δ6

(Lee et al., 2017)

tyf^UAS.Tag:FLAG/Scer\GAL4^cry.PE is a non-suppressor of abnormal locomotor rhythm | adult stage phenotype of Lsm12a^Δ6

(Lee et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^cry.PE/Lsm12a^UAS.Tag:FLAG rescues Lsm12a^Δ3

(Lee et al., 2017)

Scer\GAL4^cry.PE, Pdfr^UAS.cMa, Scer\GAL80^Pdf.PS rescues Pdfr⁵³⁰⁴

(Lear et al., 2009)

Partially rescues

Scer\GAL4^cry.PE/Pdfr^UAS.cMa partially rescues Pdfr³³⁶⁹

(Im and Taghert, 2010)

Scer\GAL4^cry.PE/Pdfr^UAS.cMa partially rescues Pdfr⁵³⁰⁴

(Lear et al., 2009)

Scer\GAL4^cry.PE, Pdfr^UAS.cMa, Scer\GAL80^Pdf.PS partially rescues Pdfr⁵³⁰⁴

(Lear et al., 2009)

per^UAS.cBa, Scer\GAL4^cry.PE, Scer\GAL80^Pdf.PS partially rescues per⁰¹

(Picot et al., 2007)

na^UAS.cLa/Scer\GAL4^cry.PE partially rescues na^har

(Lear et al., 2005)

Clk^UAS.Tag:HA/Scer\GAL4^cry.PE partially rescues Clk^Jrk

(Allada et al., 2003)

Clk^UAS.Tag:HA/Scer\GAL4^cry.PE partially rescues Clk^ar

(Allada et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^cry.PE

Name Synonyms

cryptochrome promoter construct of Emery

(Emery et al., 2000)

Secondary FlyBase IDs

References (55)

Report Sections

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