FlyBase Allele Report: Dmel\Su(H)[UAS.cKa]

General Information

Symbol

Dmel\Su(H)^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Klein

FlyBase ID

FBal0119384

Feature type

allele

Associated gene

Dmel\Su(H)

Associated Insertion(s)

Carried in Construct

P{UAS-Su(H).K}

Also Known As

UAS-Su(H)

Key Links

Transgenic product class

cDNA

(Klein et al., 2000)

wild_type

(Klein et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Klein et al., 2000)

wild_type

(Klein et al., 2000)

Mutagen

in vitro construct

(Klein et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-Su(H).K}

Cytology

Description

Expression of a 2.8kb Su(H) cDNA fragment is governed by UASt regulatory sequences.

(Klein et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Su(H)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^ptc-559.1

(Furriols and Bray, 2000)

mesothoracic tergum | medial, with Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

proneural cluster, with Scer\GAL4^klu-G410

(Klein et al., 2000)

wing | proximal, with Scer\GAL4^ptc-559.1

(Furriols and Bray, 2000)

wing disc, with Scer\GAL4^ptc-559.1

(Furriols and Bray, 2000, Klein et al., 2000)

wing margin bristle, with Scer\GAL4^klu-G410

(Klein et al., 2000)

wing vein L3, with Scer\GAL4^dpp.blk1

(Negeri et al., 2002)

Detailed Description

Statement

Reference

When Su(H)^Scer\UAS.cKa is driven by Scer\GAL4^pnr-MD237, an absence of sensory organs in the medial region of the notum is seen.

(Chandra et al., 2003)

When Su(H)^Scer\UAS.cKa is driven by Scer\GAL4^dpp.blk1, a gap or distal deletion is seen in wing vein L3.

(Negeri et al., 2002)

Wings from flies expressing Su(H)^Scer\UAS.cKa under the control of Scer\GAL4^ptc-559.1 have outgrowths in the proximal region of the wing. When the wing disc is examined areas of ectopic growth are seen at the periphery. When Su(H)^Scer\UAS.cKa is driven by Scer\GAL4^dpp.blk1 the distal L3 wing vein is eliminated.

(Furriols and Bray, 2000)

When expression is driven by Scer\GAL4^klu-G410 in a wild type background nearly all neural precursors are lost and derived bristles in the notum and wing margin are consequently missing (although proneural gene expression is initially normal in the discs). When expression is driven by Scer\GAL4^ptc-559.1 in a wild type background the wing disc shows an overgrowth phenotype similar to that caused by overexpression of Dl or 'intra' forms of N.

(Klein et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^pnr-MD237/Su(H)^UAS.cKa is a suppressor of increased cell death phenotype of Scer\GAL4^pnr-MD237, fred^RNAi.UAS

(Chandra et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^dpp.blk1, Su(H)^UAS.cKa has wing | distal phenotype, enhanceable by H^UAS.cHa, Scer\GAL4^dpp.blk1

(Furriols and Bray, 2000)

Scer\GAL4^dpp.blk1, Su(H)^UAS.cKa has wing phenotype, enhanceable by H^UAS.cHa, Scer\GAL4^dpp.blk1

(Furriols and Bray, 2000)

Scer\GAL4^ptc-559.1, Su(H)^UAS.cKa has wing | distal phenotype, enhanceable by N^55e11

(Furriols and Bray, 2000)

Scer\GAL4^dpp.blk1, Su(H)^UAS.cKa has wing | distal phenotype, enhanceable by N^55e11

(Furriols and Bray, 2000)

NOT Enhanced by

Statement

Reference

Scer\GAL4^pnr-MD237, Su(H)^UAS.cKa has mesothoracic tergum | medial phenotype, non-enhanceable by Scer\GAL4^pnr-MD237/fred^RNAi.UAS

(Chandra et al., 2003)

NOT suppressed by

Statement

Reference

Scer\GAL4^pnr-MD237, Su(H)^UAS.cKa has mesothoracic tergum | medial phenotype, non-suppressible by Scer\GAL4^pnr-MD237/fred^RNAi.UAS

(Chandra et al., 2003)

Other

Statement

Reference

Bx42^RNAi.UAS.cNa, Scer\GAL4^dpp.blk1, Su(H)^UAS.cKa has wing phenotype

(Negeri et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of fred^{dsRNA.Scer\UAS} to Su(H)^Scer\UAS.cKa, Su(H)^Scer\UAS.cKa animals has no effect on the Su(H)^Scer\UAS.cKa phenotype. The cell death phenotype seen in Su(H)^Scer\UAS.cKa animals is however, alleviated by Su(H)^Scer\UAS.cKa expression.

(Chandra et al., 2003)

When Bx42^{dsRNA.Scer\UAS} and Su(H)^Scer\UAS.cKa are driven by Scer\GAL4^dpp.blk1 a wing overgrowth phenotype is seen.

(Negeri et al., 2002)

The addition of N^55e11 to Su(H)^Scer\UAS.cKa, Scer\GAL4^dpp.blk1 or Su(H)^Scer\UAS.cKa, Scer\GAL4^ptc-559.1 flies leads to a strong enhancement of the wing nicking phenotype. The addition of H^Scer\UAS.cHa to Su(H)^Scer\UAS.cKa, Scer\GAL4^dpp.blk1 flies greatly enhances the wing nick phenotype seen in Su(H)^Scer\UAS.cKa, Scer\GAL4^dpp.blk1 flies alone. The size of the wing is greatly reduced.

(Furriols and Bray, 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^unspecified/Su(H)^UAS.cKa rescues Su(H)^del47

(Koelzer and Klein, 2003)

Partially rescues

Scer\GAL4^klu-G410/Su(H)^UAS.cKa partially rescues Su(H)⁸/Su(H)²

(Klein et al., 2000)

Scer\GAL4^dpp.blk1/Su(H)^UAS.cKa partially rescues Su(H)⁸/Su(H)²

(Klein et al., 2000)

Comments

When expression of Su(H)^Scer\UAS.cKa is driven by Scer\GAL4^dpp.blk1 in a Su(H)²/Su(H)⁸ mutant the wing pouch size defect is rescued. When expression of Su(H)^Scer\UAS.cKa is driven by Scer\GAL4^klu-G410 in a Su(H)²/Su(H)⁸ mutant the neurogenesis and wing pouch size defects are rescued.

(Klein et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Su(H)^Scer\UAS.cKa

Su(H)^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Klein

(Klein et al., 2000)

Secondary FlyBase IDs

References (5)

Report Sections

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