FlyBase Allele Report: Dmel\enok[1]

General Information

Symbol

Dmel\enok¹

Species

D. melanogaster

Name

FlyBase ID

FBal0121785

Feature type

allele

Associated gene

Dmel\enok

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Scott et al., 2001)

Progenitor genotype

Cytology

Description

The mutation is in the HAT domain at the end of the zinc finger motif.

(Scott et al., 2001)

Amino acid replacement: W?term.

(Scott et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:24,102,293..24,102,293 [+]

Nucleotide change:

G24102293A

Amino acid change:

W765term | enok-PA; W765term | enok-PB

Reported amino acid change:

W?term

Comment:

Reported as a W to amber mutation. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Scott et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult mushroom body | somatic clone

(Scott et al., 2001)

egg chamber | germline clone | oogenesis | progressive

(Scott et al., 2001)

embryonic/larval neuron | larval stage

(Scott et al., 2001)

female germline stem cell | germline clone

(Xin et al., 2013)

germarium cap cell | progressive | somatic clone

(Xin et al., 2013)

Detailed Description

Statement

Reference

Marked enok¹ mutant female germline stem cells (GSCs) are present only 2 days after clone induction (ACI), but absent at 21 days ACI. Control clones are present at 21 days ACI. The mutant cells differentiate into marked mutant cysts. The cells in the mutant clones do not display signs of apoptosis.

Tracing of homozygous enok¹ mutant cap cells in mosaic germaria for 21 days after eclosion reveals that the proportion of mutant clones drops dramatically over the time course, while the percentage the control ones remains steady.

Females heterozygous for enok¹ have normal germarium cap cell numbers.

(Xin et al., 2013)

Homozygous somatic clones in the wing disc are not significantly different in size to wild type controls.

(Scott et al., 2001)

Homozygotes tend to enter the wandering third instar larval stage at about 7 days after larval hatching (ALH) at 25^oC while their heterozygous counterparts take only 3.5 to 4.0 days. Approximately half of the animals die before pupariation formation. Those that pupate do so at around 10 days ALH. Homozygotes never eclose as adults. Homozygous mutant clones exhibit defects in the mushroom body. Mushroom bodies contain only what appears to be a γ lobe and have a reduced cell number. The clones stop adding neurons to the mushroom body by 2.5 days ALH. When mitotic recombination is induced at different times the normal progression through γ, α'/β', and finally α/β neurons is seen and that transitions between cell types occur at normal times. Branched α'/β', and α/β can be generated in late-induced enok¹ clones. enok¹ clones in the wing disc show no proliferation defects. Throughout the life of females generating germ-line clone homozygous enok¹ eggs, a slow but steady decline in the number of eggs laid is seen when compared to wild-type.

(Scott et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

enok¹/enok[+] is an enhancer of homeotic | adult stage phenotype of trx¹

(Umer et al., 2019)

enok¹/enok[+] is an enhancer of homeotic | adult stage phenotype of trx^E2

(Umer et al., 2019)

Suppressor of

Statement

Reference

enok¹/enok[+] is a suppressor of homeotic | adult stage phenotype of Pc¹

(Umer et al., 2019)

enok¹/enok[+] is a suppressor of homeotic | adult stage phenotype of Pc¹¹

(Umer et al., 2019)

Phenotype Manifest In

Enhancer of

Statement

Reference

enok¹/enok[+] is an enhancer of adult abdominal segment 4 phenotype of trx¹

(Umer et al., 2019)

enok¹/enok[+] is an enhancer of adult abdominal segment 5 phenotype of trx¹

(Umer et al., 2019)

enok¹/enok[+] is an enhancer of adult abdominal segment 4 phenotype of trx^E2

(Umer et al., 2019)

enok¹/enok[+] is an enhancer of adult abdominal segment 5 phenotype of trx^E2

(Umer et al., 2019)

enok¹/enok[+] is an enhancer of germarium cap cell phenotype of N^264-39

(Xin et al., 2013)

NOT Enhancer of

Statement

Reference

enok¹/enok[+] is a non-enhancer of adult thorax | pharate adult stage phenotype of chm¹⁴

(Miotto et al., 2006)

Suppressor of

Statement

Reference

enok¹/enok[+] is a suppressor of sex comb | ectopic phenotype of Pc¹

(Umer et al., 2019)

enok¹/enok[+] is a suppressor of sex comb | ectopic phenotype of Pc¹¹

(Umer et al., 2019)

NOT Suppressor of

Statement

Reference

enok¹/enok[+] is a non-suppressor of adult thorax | pharate adult stage phenotype of chm¹⁴

(Miotto et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The germarium cap cell loss caused by N^264-39/+ is significantly enhanced by enok¹/+.

(Xin et al., 2013)

Throughout the life of females generating germ-line clone homozygous enok¹ eggs, a slow but steady decline in the number of eggs laid is seen when compared to wild-type.

(Scott et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

enok¹ is partially rescued by enok^+tSa

(Scott et al., 2001)

enok¹ is partially rescued by enok^T35

(Scott et al., 2001)

enok¹ is partially rescued by enok^+tSa

(Scott et al., 2001)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

6284

y¹ w^*; cn¹ bw¹ enok¹/SM6b

Kyoto

108718

y¹ w^*; cn¹ bw¹ enok¹/SM6b

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Enok¹

(Kim et al., 2023)

enok¹

(Umer et al., 2019, Xin et al., 2013)

enok^m1

(Scott et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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