Overexpression of Uba2C175S.Scer\UAS driven by Scer\GAL4GMR.PF results in a variable slightly glossy, rough eye phenotype, sometimes with loss of pigmentation. This is temperature-dependent.
Expression of one copy of Uba2C175S.Scer\UAS under the control of Scer\GAL4elav-C155 generally results in the production of apparently normal adult progeny with no or very mild lethality at 29oC. P{UAS-Uba2.C175S} lines expressing higher levels of Uba2C175S.Scer\UAS result in higher lethality (approximately 50% for P{UAS-Uba2.C175S}117F and 100% for P{UAS-Uba2.C175S}92E). Animals expressing high levels of Uba2C175S.Scer\UAS under the control of Scer\GAL4GMR.PF have a rough, depigmented eye.
Scer\GAL4GMR.PF, Uba2C175S.UAS has increased cell death | temperature conditional phenotype, enhanceable by Hsap\ARQ112.UAS.Tag:HA, Scer\GAL4GMR.PF
Hsap\ARQ112.UAS.Tag:HA, Scer\GAL4GMR.PF, Uba2C175S.UAS has increased cell death | temperature conditional phenotype, non-suppressible by Hsap\HSPA1LUAS.cWa, Scer\GAL4GMR.PF
Uba2C175S.UAS, Scer\GAL4GMR.PU is an enhancer of visible phenotype of Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PU, Zzzz\CAG127Q.UAS.Tag:HA
Uba2C175S.UAS, Scer\GAL4GMR.PU is an enhancer of visible phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.PU
Uba2C175S.UAS/Scer\GAL4GMR.PU is an enhancer of visible phenotype of Scer\GAL4GMR.PU, Zzzz\CAG127Q.UAS.Tag:HA
Uba2C175S.UAS, Scer\GAL4GMR.PU is a non-enhancer of visible phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PU
Uba2C175S.UAS, Scer\GAL4GMR.PU is a non-suppressor of visible phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PU
Scer\GAL4GMR.PF, Uba2C175S.UAS has eye | temperature conditional phenotype, enhanceable by Hsap\ARQ112.UAS.Tag:HA, Scer\GAL4GMR.PF
Scer\GAL4GMR.PF, Uba2C175S.UAS has ommatidium | temperature conditional phenotype, enhanceable by Hsap\ARQ112.UAS.Tag:HA, Scer\GAL4GMR.PF
Hsap\ARQ112.UAS.Tag:HA, Scer\GAL4GMR.PF, Uba2C175S.UAS has eye phenotype, non-suppressible by Hsap\HSPA1LUAS.cWa, Scer\GAL4GMR.PF
Hsap\ARQ112.UAS.Tag:HA, Scer\GAL4GMR.PF, Uba2C175S.UAS has eye | temperature conditional phenotype, non-suppressible by Hsap\HSPA1LUAS.cWa, Scer\GAL4GMR.PF
Hsap\ARQ112.UAS.Tag:HA, Scer\GAL4GMR.PF, Uba2C175S.UAS has ommatidium | temperature conditional phenotype, non-suppressible by Hsap\HSPA1LUAS.cWa, Scer\GAL4GMR.PF
Uba2C175S.UAS, Scer\GAL4GMR.PU is an enhancer of ommatidium phenotype of Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PU, Zzzz\CAG127Q.UAS.Tag:HA
Uba2C175S.UAS, Scer\GAL4GMR.PU is an enhancer of ommatidium phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.PU
Uba2C175S.UAS/Scer\GAL4GMR.PU is an enhancer of ommatidium phenotype of Scer\GAL4GMR.PU, Zzzz\CAG127Q.UAS.Tag:HA
Uba2C175S.UAS, Scer\GAL4GMR.PF is an enhancer of eye phenotype of Hsap\ARQ112.UAS.Tag:HA, Scer\GAL4GMR.PF
Uba2C175S.UAS, Scer\GAL4GMR.PU is a non-enhancer of ommatidium phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PU
Uba2C175S.UAS, Scer\GAL4GMR.PU is a non-suppressor of ommatidium phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Hsp60DRNAi.Sym.UAS, Scer\GAL4GMR.PU
The eye phenotype resulting from simultaneous expression of Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 and Hsp60DdsRNA.Sym.Scer\UAS via Scer\GAL4GMR.PU does not appear to be affected by co-expression of Uba2C175S.Scer\UAS.
The Hsp60DdsRNA.Sym.Scer\UAS-mediated suppression of the eye phenotype resulting from the expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 driven by Scer\GAL4GMR.PU is less pronounced in the presence of Uba2C175S.Scer\UAS.
Simultaneous expression of Uba2C175S.Scer\UAS and Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 via Scer\GAL4GMR.PU enhances eye degradation, resulting in more glazed eyes with little pigmentation.
Simultaneous expression of Uba2C175S.Scer\UAS and Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 via Scer\GAL4GMR.PU enhances the eye degeneration phenotype.
Co-expression of Uba2C175S.Scer\UAS with Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 (both under the control of Scer\GAL4GMR.PF) results in a constant enhancement of the eye degeneration, as illustrated by severe loss of pigmentation.
Co-expression of Uba2C175S.Scer\UAS and Hsap\HSPA1LScer\UAS.cWa with Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 (all under the control of Scer\GAL4GMR.PF) fails to suppress the enhancement of the eye degeneration phenotype found in Uba2C175S.Scer\UAS ; Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 flies.