FlyBase Allele Report: Dmel\hh[C84S.UAS]

General Information

Symbol

Dmel\hh^C84S.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0122970

Feature type

allele

Associated gene

Dmel\hh

Associated Insertion(s)

Carried in Construct

P{UAS-hh.C84S}

Key Links

Transgenic product class

missense_variant

(Lee et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Lee et al., 2001)

Mutagen

in vitro construct

(Lee et al., 2001)

Progenitor genotype

hh^UAS.cLa

(Lee et al., 2001)

Carried in construct

P{UAS-hh.C84S}

Cytology

Description

Construct: Nucleotide substitution: G?C. Amino acid replacement: C84S.

(Lee et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^bi-omb-Gal4

(Lee et al., 2001)

visible, with Scer\GAL4^en-e16E

(Lee et al., 2001)

visible, with Scer\GAL4^en-e16E, hh^ts2

(Lee et al., 2001)

visible, with Scer\GAL4^ey.PH

(Lee et al., 2001)

visible, with Scer\GAL4^ptc-559.1

(Lee et al., 2001)

Phenotype Manifest In

1st posterior cell, with Scer\GAL4^bi-omb-Gal4

(Lee et al., 2001)

1st posterior cell, with Scer\GAL4^en-e16E

(Lee et al., 2001)

1st posterior cell, with Scer\GAL4^en-e16E, hh^ts2

(Lee et al., 2001)

anterior crossvein, with Scer\GAL4^ptc-559.1

(Lee et al., 2001)

ocellus, with Scer\GAL4^ey.PH

(Lee et al., 2001)

wing vein L3, with Scer\GAL4^ptc-559.1

(Lee et al., 2001)

wing vein L4, with Scer\GAL4^ptc-559.1

(Lee et al., 2001)

Detailed Description

Statement

Reference

Expression of hh^{C84S.Scer\UAS} under the control of Scer\GAL4^bi-omb-Gal4 or Scer\GAL4^en-e16E results in a reduction or loss of the L3-L4 intervein region. Expression of hh^{C84S.Scer\UAS} under the control of Scer\GAL4^en-e16E at 29^oC in flies which are heterozygous for hh^ts2 results in an enhancement of the loss of the L3-L4 intervein region. Expression of hh^{C84S.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 results in fusion of wing veins L3 and L4 proximally and loss of the anterior crossvein. Expression of hh^{C84S.Scer\UAS} under the control of Scer\GAL4^ey.PH blocks the formation of ocelli.

(Lee et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scer\GAL4^ap-md544, Sxl^M3.UAS, hh^C84S.UAS has lethal | pupal stage phenotype

(Horabin, 2005)

Phenotype Manifest In

Other

Statement

Reference

Scer\GAL4^ap-md544, Sxl^M3.UAS, hh^C84S.UAS has wing phenotype

(Horabin, 2005)

Scer\GAL4^ap-md544, Sxl^M3.UAS, hh^C84S.UAS has wing disc phenotype

(Horabin, 2005)

Scer\GAL4^ap-md544, Sxl^M3.UAS, hh^C84S.UAS has wing cell phenotype

(Horabin, 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Male and female wing discs, when expressing Sxl^M3.Scer\UAS and hh^{C84S.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 show a change in their normal proportions that is suggestive of enlargement. All male discs show the posterior en signal with an anterior extension, with the rest of the anterior compartment relatively normal in size. In females, the anterior compartment is larger than the posterior, frequently by almost twofold.

Co-expressing Sxl^M3.Scer\UAS and hh^{C84S.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 is essentially lethal to both males and females; rare female escapers have misshapen wings with bubbles and a narrowing of the L3 and L4 intervein region (this is also observed in hh^{C84S.Scer\UAS}-expressing flies).

(Horabin, 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^en-e16E/hh^C84S.UAS fails to rescue hh^AC/hh^ts2

(Lee et al., 2001)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

hh^{C84S.Scer\UAS}

hh^C84S.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close