FlyBase Allele Report: Dmel\Pdk1[EP3553]

General Information

Symbol

Dmel\Pdk1^EP3553

Species

D. melanogaster

Name

FlyBase ID

FBal0123127

Feature type

allele

Associated gene

Dmel\Pdk1

Associated Insertion(s)

P{EP}Pdk1^EP3553

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Kraut et al., 2001, Kraut et al., 2001)

Progenitor genotype

Associated Insertion(s)

P{EP}Pdk1^EP3553

Cytology

Description

The P{EP} insertion is 179bp upstream of the Pk61C transcription start site.

(Cho et al., 2001)

The P{EP} element is inserted 35 nucleotides 5' to the start of the 2nd alternative 5' exon.

(Kraut et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:131,797..131,797 [+]

(Jones et al., 2009, Chen et al., 2003, Gene Disruption Project members, 2001-, Kraut et al., 2001, Kraut et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

Phenotype Manifest In

anterior fascicle & synapse, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

anterior fascicle & synapse | supernumerary, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

eye, with Scer\GAL4^GMR.PF

(Chen et al., 2003)

larval intersegmental nerve, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

Detailed Description

Statement

Reference

Age-related locomotor impairment is significantly delayed in mutant females compared to controls.

Mutant males and females show normal levels of resistance to paraquat.

(Jones et al., 2009)

Pk61C^EP3553 driven by Scer\GAL4^GMR.PF leads to enlarged eyes.

(Chen et al., 2003)

In mutant larvae expressing Pk61C^EP3553 under the control of Scer\GAL4^elav-C155, abnormal ISN branches are observed; the 6/7 synapse appears to be doubles. Mutant larvae expressing Pk61C^EP3553 under the control of Scer\GAL4^elav-C155 have ISN neuron pathfinding defects. Mutant larvae expressing Pk61C^EP3553 under the control of Scer\GAL4^elav-C155 have excessive or ectopic synapses.

(Kraut et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Pdk1^EP3553, Scer\GAL4^ptc-559.1 is an enhancer of 1st posterior cell phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^ptc-559.1

(Cho et al., 2001)