FlyBase Allele Report: Dmel\spoon[EP1400]

General Information

Symbol

Dmel\spoon^EP1400

Species

D. melanogaster

Name

FlyBase ID

FBal0123301

Feature type

allele

Associated gene

Dmel\spoon

Associated Insertion(s)

P{EP}EP1400

P{unk}EP1400-P2

Carried in Construct

Also Known As

EP1400

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-, Kraut et al., 2001, Kraut et al., 2001)

Progenitor genotype

Associated Insertion(s)

P{EP}EP1400

P{unk}EP1400-P2

Cytology

Description

Two insertions are present in this line: the P{EP}spoon^EP1400 insertion within the first intron and a second insertion, P{unk}spoon^EP1400-P2, approximately 1580bp distal to the first insertion.

(Hadad et al., 2011)

The P{EP} element is inserted within the first intron, upstream of the entire coding region.

(Kraut et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:5,420,297..5,420,297 [-]

(Tripathi et al., 2017, Tripathi et al., 2016, Hadad et al., 2011, Gene Disruption Project members, 2001-, Kraut et al., 2001, Kraut et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN8OS^CTG112.UAS

(Mutsuddi et al., 2004)

exacerbates spinocerebellar ataxia type 8

modeled by Hsap\ATXN8OS^CTG112.UAS

(Tripathi et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

Phenotype Manifest In

anterior fascicle & synapse, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

larval intersegmental nerve, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

Detailed Description

Statement

Reference

Mutant larvae expressing CG3249^EP1400 under the control of Scer\GAL4^elav-C155 have reduced or abnormal synapses. Mutant larvae expressing CG3249^EP1400 under the control of Scer\GAL4^elav-C155 have ISN neuron pathfinding defects.

(Kraut et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

spoon^EP1400, Scer\GAL4^GMR.PF is a suppressor of abnormal neuroanatomy phenotype of Hsap\ATXN8OS^CTG112.UAS, Scer\GAL4^GMR.PF

(Mutsuddi et al., 2004)

Phenotype Manifest In

Suppressor of

Statement

Reference

spoon^EP1400, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Hsap\ATXN8OS^CTG112.UAS, Scer\GAL4^GMR.PF

(Mutsuddi et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The disorganization of the adult eye ommatidial lattice, disarrayed axonal projection in the optic nerve along with the increased cell death of developing photoreceptors in larval eye discs characteristic for flies expressing Hsap\ATXN8OS^{CTG112.Scer\UAS} under the control of Scer\GAL4^GMR.PU is further worsened by spoon^KG02745 homozygosity.

(Tripathi et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

11236

w¹¹¹⁸ P{EP}EP1400 P{unk}EP1400-P2 spoon^EP1400

Kyoto

101211