FlyBase Allele Report: Dmel\Scr[UAS.cMa]

General Information

Symbol

Dmel\Scr^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Miller

FlyBase ID

FBal0124001

Feature type

allele

Associated gene

Dmel\Scr

Associated Insertion(s)

Carried in Construct

P{UAS-Scr.M}

Also Known As

UAS-Scr

Key Links

Transgenic product class

cDNA

(Miller et al., 2001)

wild_type

(Miller et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Miller et al., 2001)

wild_type

(Miller et al., 2001)

Mutagen

in vitro construct

(Miller et al., 2001, Heuer et al., 1995)

Progenitor genotype

Carried in construct

P{UAS-Scr.M}

Cytology

Description

UAS sequences drive expression of a 1.2kb BamHI/MluI fragment of the Scr L3 cDNA.

(Miller et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Scr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

antenna, with Scer\GAL4^dpp.blk1

(Abzhanov et al., 2001)

arista, with Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

embryonic/larval midgut primordium, with Scer\GAL4^how-24B

(Miller et al., 2001)

embryonic gastric caecum & parasegment 3, with Scer\GAL4^how-24B

(Miller et al., 2001)

embryonic gastric caecum & parasegment 7, with Scer\GAL4^how-24B

(Miller et al., 2001)

embryonic midgut chamber 2, with Scer\GAL4^how-24B

(Miller et al., 2001)

embryonic tritocerebrum, with Scer\GAL4^sca-537.4

(Sprecher et al., 2004)

eye | pharate adult stage, with Scer\GAL4^ey.PU

(Javeed et al., 2015)

eye | pupal stage, with Scer\GAL4^ey.PU

(Javeed et al., 2015)

gastric caecum primordium, with Scer\GAL4^how-24B

(Miller et al., 2001)

head | pharate adult stage, with Scer\GAL4^ey.PU

(Javeed et al., 2015)

head | pupal stage, with Scer\GAL4^ey.PU

(Javeed et al., 2015)

larval abdominal ganglion Leucokinin neuron | ectopic | first instar larval stage, with Scer\GAL4^elav-C155

(Estacio-Gómez et al., 2013)

larval thoracic ventral intersegmental muscle cell, with Scer\GAL4^how-24B

(Miller et al., 2001)

larval thoracic ventral intersegmental muscle cell | ectopic, with Scer\GAL4^how-24B

(Miller et al., 2001)

larval ventral nerve cord | first instar larval stage, with Scer\GAL4^elav-C155

(Estacio-Gómez et al., 2013)

metathoracic ventral acute muscle cell | ectopic, with Scer\GAL4^how-24B

(Miller et al., 2001)

pretarsus | ectopic, with Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

tarsal segment, with Scer\GAL4^dpp.blk1

(Abzhanov et al., 2001)

wing, with Scer\GAL4^Bx-MS1096

(Javeed et al., 2015)

Detailed Description

Statement

Reference

The wings of flies expressing Scr^Scer\UAS.cMa under the control of Scer\GAL4^Bx-MS1096 always fail to unfold after eclosion.

Expression of Scr^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PU results in a severe rough eye phenotype.

Flies expressing Scr^Scer\UAS.cMa under the control of Scer\GAL4^ey.PU dies as pupae or pharate adult, with no eyes and severely reduced head.

(Javeed et al., 2015)

Expression of Scr^Scer\UAS.cMa under the control of Scer\GAL4^dpp.blk1 results in transformation of the arista to tarsus.

(Percival-Smith et al., 2005)

Expression of Scr^Scer\UAS.cMa under the control of Scer\GAL4^sca-537.4 results in a mutant phenotype in the embryonic tritocerebrum. The phenotype has a penetrance of more than 70-80%.

(Sprecher et al., 2004)

Animals expressing Scr^Scer\UAS.cMa under the control of Scer\GAL4^dpp.blk1 have morphologically normal legs, while the antennae are transformed distally to a tarsus-like identity.

(Abzhanov et al., 2001)

Expression of Scr^Scer\UAS.cMa under the control of Scer\GAL4^lab.PH does not result in morphological defects in the tritocerebrum or any other part of the embryonic brain.

(Hirth et al., 2001)

When Scr^Scer\UAS.cMa is driven by Scer\GAL4^how-24B, repressed posterior central projections (PVs) are seen in T3 and A1 and an absent ventral oblique muscle 2 (VO2) in T2. Occasionally these animals produce ectopic anterior ventral projections (AVs) in T2 and T3 which exemplifies T1 character. Occasionally an ectopic PV is seen in T2. Midgut morphology is altered with reduced gastric caecae at parasegment 3 and occasionally a reduced first constriction. Polyps that resemble ectopic gastric caecum primordia appear to form on the second midgut chamber, but fail to develop further. Midgut morphology is also altered in parasegment 7.

(Miller et al., 2001)

When driven by Scer\GAL4^69B, Scer\GAL4^prd.RG1, or Scer\GAL4^{l(3)31-1-31-1} leads to a significant loss in viability.

(Miller et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^dpp.blk1, Scr^UAS.cMa has visible phenotype, suppressible by pb^UAS.EGFP/Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^dpp.blk1, Scr^UAS.cMa has arista phenotype, suppressible by pb^UAS.EGFP/Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

Scer\GAL4^dpp.blk1, Scr^UAS.cMa has pretarsus | ectopic phenotype, suppressible by pb^UAS.EGFP/Scer\GAL4^dpp.blk1

(Percival-Smith et al., 2005)

Suppressor of

Statement

Reference

Scer\GAL4^lab.PH/Scr^UAS.cMa is a suppressor of embryonic tritocerebrum phenotype of lab¹⁴

(Hirth et al., 2001)

Other

Statement

Reference

Dfd¹⁶, Scer\GAL4^salm-459.2, Scr⁴, Scr^UAS.cMa has adult corpus allatum phenotype

(Sánchez-Higueras et al., 2014)

Dfd¹⁶, Scer\GAL4^salm-459.2, Scr⁴, Scr^UAS.cMa has prothoracic gland phenotype

(Sánchez-Higueras et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Addition of Scr^Scer\UAS.cMa to Dfd¹⁶;Scr⁴ double mutant embryos (under the control of Scer\GAL4^salm-459.2) results in both the Mx and Lb segments forming prothoracic glands.

(Sánchez-Higueras et al., 2014)

Co-expression of pb^{Scer\UAS.T:Avic\GFP-EGFP} suppresses the arista-to-tarsus transformation seen in flies expressing Scr^Scer\UAS.cMa under the control of Scer\GAL4^dpp.blk1.

(Percival-Smith et al., 2005)

Expression of Scr^Scer\UAS.cMa under the control of Scer\GAL4^lab.PH rescues the tritocerebral defects seen in lab¹⁴ embryonic brains. 36.4% of embryos show a complete rescue of the defects (taking into account that the phenotypic penetrance of the lab¹⁴ phenotype is 88.6%).

(Hirth et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7302

w¹¹¹⁸; P{UAS-Scr.M}EE2/TM6B, Tb⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Scr^Scer\UAS.cKa

Scr^Scer\UAS.cMa

Scr^UAS.cKa

Scr^UAS.cMa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Miller

(Miller et al., 2001)

Secondary FlyBase IDs

FBal0045051

References (18)

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