FB2024_03 , released June 25, 2024
Allele: Dmel\Gαs+t10
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General Information
Symbol
Dmel\Gαs+t10
Species
D. melanogaster
Name
FlyBase ID
FBal0126533
Feature type
allele
Associated gene
Dmel\Gαs
Associated Insertion(s)
Carried in Construct
P{Gαs+t10}
Also Known As
Gs27
Key Links
Genomic Maps

Transgenic product class
genomic_DNA
(Wolfgang et al., 2001)
wild_type
(Wolfgang et al., 2001)
Mutagen
Nature of the Allele
Transgenic product class
genomic_DNA
(Wolfgang et al., 2001)
wild_type
(Wolfgang et al., 2001)
Mutagen
in vitro construct
(Wolfgang et al., 2001)
Progenitor genotype
Carried in construct
P{Gαs+t10}
Cytology
Description

10kb BamHI genomic fragment containing the entire Gαs gene and roughly 2kb 5' to the transcription initiation site and 2kb 3' to the translational stop site.

(Wolfgang et al., 2001)
Allele components
Component
Use(s)
Regulatory region(s)
Gαs
Encoded product / tool
Gαs
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
rescue_region
2R:23,940,746..23,950,004 [+]
Linked to:
BamHI-BamHI restriction fragment
Comment:

Position of restriction fragment on reference sequence inferred by FlyBase curator.

(Wolfgang et al., 2001)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Gαs+t10 rescues GαsB19

      (Wolfgang et al., 2004)

      Gαs+t10 rescues GαsR79

      (Wolfgang et al., 2001)

      Gαs+t10 rescues GαsR65

      (Wolfgang et al., 2001)

      Gαs+t10 rescues GαsR67

      (Wolfgang et al., 2001)

      Gαs+t10 rescues GαsR33

      (Wolfgang et al., 2001)

      Gαs+t10 rescues GαsB19

      (Wolfgang et al., 2001)

      Gαs+t10 rescues GαsR60

      (Wolfgang et al., 2001)
      Partially rescues

      Gαs+t10 partially rescues GαsR60

      (Hou et al., 2003)
      Comments

      Expression of one copy of G-sα60A+t10 is able to rescue some aspects of the synaptic impairment in G-sα60AR60 homozygote mutant embryos, while it fails to rescue other aspects. For example, G-sα60A+t10 partially rescues the synaptic facilitation during tetanic stimulation defect, it enhances the asynchronous transmitter release during and after tetanus and it increases the frequency and amplitude of mSCs. However, embryos expressing G-sα60A+t10 still show a failure to evoke synaptic currents in response to nerve stimulation, they still lack post-tetanic potentiation and still exhibit a skewed amplitude distribution.

      (Hou et al., 2003)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      G-sα60A+t10
      Gαs+t10
      Name Synonyms
      Secondary FlyBase IDs
        References (3)