FB2024_03 , released June 25, 2024
Allele: Dmel\Gr5aΔEP19
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General Information
Symbol
Dmel\Gr5aΔEP19
Species
D. melanogaster
Name
FlyBase ID
FBal0127248
Feature type
allele
Associated gene
Dmel\Gr5a
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
Delta2-3 transposase
(Ueno et al., 2001)
Progenitor genotype
P{EP}Tre1EP496
(Ueno et al., 2001)
Caused by aberration
Df(1)TreΔEP19
Cytology
Description

Proximal breakpoint lies within the second exon of Gr5a, 3' to the codon specifying Ala 62. The translation initiation codon of Gr5a is removed.

(Dahanukar et al., 2001)

Imprecise excision of the progenitor insertion, resulting in a 1.0kb deletion of genomic sequences in the direction of Gr5a (removing the promoter, 5' leader sequences and a 253bp sequence removing exon 1, intron 1 and part of exon 2). The 3' end of the progenitor insertion has been precisely excised, leaving the Tre1 gene structure intact.

(Ueno et al., 2001)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
X:5,679,980..5,680,979 [+]
Comment:

1000 bp deletion associated with the precise excision of P{EP}Tre1EP496 (GB:AB066612).

(Ueno et al., 2001)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
AB066612
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Stimulation of long labellar taste bristles in mutant flies with trehalose induces significantly fewer biphasic spikes than seen in wild-type.

      Stimulation of long labellar taste bristles in TreΔEP19 mutant flies with trehalose induces significantly fewer biphasic spikes than seen in wild-type.

      (Ueno et al., 2006)

      Impulse discharges from I-type hairs in response to trehalose stimulation are diminished in Gr5aΔEP19 mutants compared to wild-type.

      (Usui-Aoki et al., 2005)

      Electrophysiological response to sugars in individual labellar taste hairs of the large and medium class shows normal response to sucrose but drastically reduced response to trehalose, in comparison with parental P{EP}Tre1EP496 strain. In a two choice preference test with sucrose and trehalose, mutants show dramatically reduced response to trehalose, when compared to the parental P{EP}Tre1EP496 strain.

      (Dahanukar et al., 2001)

      Flies show reduced sensitivity to trehalose in a feeding preference test.

      (Ueno et al., 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      Gr5a01

      (Ueno et al., 2001)
      Rescued by

      Gr5aΔEP19 is rescued by Gr5a+t10

      (Dahanukar et al., 2001)
      Not rescued by

      Gr5aΔEP19 is not rescued by Gr5aSTOP

      (Dahanukar et al., 2001)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 1 )
      Crossreferences
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      EP(X)-19
      (Usui-Aoki et al., 2005)
      Gr5aΔEP19
      (Ueno et al., 2006)
      TreΔEP19
      ΔEP(X)-19
      (Dahanukar et al., 2001)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)