FlyBase Allele Report: Dmel\ash2[S112411]

General Information

Symbol

Dmel\ash2^S112411

Species

D. melanogaster

Name

FlyBase ID

FBal0129432

Feature type

allele

Associated gene

Dmel\ash2

Associated Insertion(s)

P{lacW}ash2^S112411

Carried in Construct

Also Known As

ash2¹¹²⁴¹¹

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Deak et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{lacW}ash2^S112411

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal

(Amoros et al., 2002)

partially lethal - majority die | recessive

(Angulo et al., 2004, Deak et al., 1997)

some die during pharate adult stage | recessive

(Angulo et al., 2004)

sterile

(Amoros et al., 2002)

sterile | recessive

(Angulo et al., 2004)

visible

(Beltran et al., 2007)

Phenotype Manifest In

Detailed Description

Statement

Reference

Wings are reduced in size compared to controls in mutant adults. Dorsal bristles in the mutant wing margin are partially ventralised.

(Beltran et al., 2007)

Most ash2^S112411 homozygotes die as pharate adults. Escapers are sterile, have reduced wing size, a crooked L2 and ectopic vein tissue, mainly extra cross-veins and thicker veins. Late third instar wing discs in these mutants are smaller than wild-type.

(Angulo et al., 2004)

About 12% of homozygotes develop into sterile adults that survive for two days. These files show an anomalous pattern of differentiation of the appendages, often associated with homeotic transformations. One example of this is a transformation of the first to the second thoracic segment as observed by reduction of the sex comb teeth, and the occasional partial transformation of the haltere to wing. Mutant wings contain supernumerary cross-veins with 95% penetrance, they are seen in the proximal regions between wing veins L2 and L3, and L4 and L5. Differentiation elements such as longitudinal veins are normally distributed and an overall reduction in wing size is observed. Occasionally other phenotypes are seen: Notches along the posterior margin, winder L3 and L5 wing veins, and distorted L2 veins.

(Amoros et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

ash2^S112411 has wing phenotype, enhanceable by Egfr^E3/Egfr[+]

(Angulo et al., 2004)

ash2^S112411 has wing vein | ectopic phenotype, enhanceable by Egfr^E3/Egfr[+]

(Angulo et al., 2004)

ash2^S112411 has submarginal cell phenotype, enhanceable by bs⁰³²⁶⁷/bs[+]

(Angulo et al., 2004)

ash2^S112411 has discal cell phenotype, enhanceable by bs⁰³²⁶⁷/bs[+]

(Angulo et al., 2004)

ash2^S112411 has wing vein | ectopic phenotype, enhanceable by bs⁰³²⁶⁷/bs[+]

(Angulo et al., 2004)

ash2^S112411 has crossvein | ectopic phenotype, enhanceable by bs⁰³²⁶⁷/bs[+]

(Angulo et al., 2004)

ash2^S112411 has wing vein L2 phenotype, enhanceable by bs⁰³²⁶⁷/bs[+]

(Angulo et al., 2004)

NOT suppressed by

Statement

Reference

ash2^S112411 has crossvein | ectopic phenotype, non-suppressible by cv-2¹

(Amoros et al., 2002)

Enhancer of

Statement

Reference

ash2^S112411/ash2^S112411 is an enhancer of wing vein phenotype of Egfr^E3

(Angulo et al., 2004)

ash2^S112411/ash2^S112411 is an enhancer of wing phenotype of Egfr^E3

(Angulo et al., 2004)

Suppressor of

Statement

Reference

ash2^S112411/ash2^S112411 is a suppressor | partially of wing vein phenotype of rho^ve-1, vn¹

(Angulo et al., 2004)

ash2^S112411/ash2^S112411 is a suppressor of wing vein L2 phenotype of kni^ri-1

(Angulo et al., 2004)

ash2^S112411 is a suppressor of crossvein phenotype of cv-2¹

(Amoros et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

rho^ve-1 vn¹ double homozygotes lack wing veins. This phenotype is partially suppressed by ash2^S112411 /ash2^S112411. The degree of suppression varies from development of L2 only to almost complete rescue, albeit with some abnormalities such as extra crossvein material or proximal fusions between L2 and L3 or L4 and L5. In 25% of cases, hollow, tube like wings are formed, probably due to detachment of dorsal and ventral layers.

Egfr^E3/+ adults have extra vein tissue at the distal end of L2. This phenotype is enhanced by ash2^S112411/ash2^S112411 - the resulting wings are more reduced and have more ectopic veination than ash2^S112411/ash2^S112411 animals.

Heterozygous bs⁰³²⁶⁷ flies have small patches of ectopic vein in the vicininty of L2 and L5. If these flies are also homozygous for ash2^S112411 their wings are small, blistered and exhibit localised reduction and loss of intervein betwee L2 and L3 and between L4 and L5 and ectopic crossveins. Fusions occur between L2 and L3 and between L4 and L5, particularly proximally.

ash2^S112411/ash2^S112411 suppresses the loss of L2 seen in kni^ri-1/kni^ri-1 flies.

(Angulo et al., 2004)

cv-2¹/cv-2¹, ash2^S112411/+ flies have normal crossveins. cv-2¹/cv-2¹, ash2^S112411/ash2^S112411 have extra crossveins and reduction in wing size.

(Amoros et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ash2^S112411 is rescued by ash2^UAS.Tag:HA/Scer\GAL4^nub-AC-62

(Beltran et al., 2007)

ash2^S112411 is rescued by ash2^UAS.Tag:HA/Scer\GAL4^Bx-MS1096

(Beltran et al., 2007)

Comments

The reduced wing size seen in ash2^S112411 adults is rescued by expression of ash2^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^nub-AC-62.

Expression of ash2^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Bx-MS1096 rescues the partial ventralisation of the dorsal wing margin bristles which is seen in ash2^S112411 mutants.

(Beltran et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Mutant phenotypes are revertable by excision of the P-element.

(Amoros et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

1124/11

(Deak et al., 1997)

ash2¹¹²⁴¹¹

(Beltran et al., 2007, Amoros et al., 2002)

ash2^S112411

l(3)S112411^S112411

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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