FB2024_03 , released June 25, 2024
Allele: Dmel\GαqRNAi.UAS
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General Information
Symbol
Dmel\GαqRNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0131024
Feature type
allele
Associated gene
Dmel\Gαq
Associated Insertion(s)
Carried in Construct
P{UAS-Gαq-RNAi}
Key Links
Transgenic product class
RNAi_reagent
(Kalidas and Smith, 2002)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Kalidas and Smith, 2002)
Mutagen
in vitro construct
(Kalidas and Smith, 2002)
Progenitor genotype
Carried in construct
P{UAS-Gαq-RNAi}
Cytology
Description

UASt sequences drive expression of genomic Gαq sequences (corresponding to exons three four and five and the splice acceptor for exon 6A), and fused with an inverted Gαq cDNA fragment corresponding to the same region.

(Kalidas and Smith, 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Gαq
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Gα49BdsRNA.Scer\UAS under the control of Scer\GAL4Orco.T:Hsim\VP16 does not reduce the odour response of the ab1A or ab2A olfactory receptor neurons (tested with ethyl acetate) or of the ab3A ORN (tested with methyl butyrate).

      Expression of Gα49BdsRNA.Scer\UAS under the control of Scer\GAL4Gr21a.9.323 results in a significant decrease in the response of the ab1C ORN to CO[[2]], across a wide response range.

      (Yao and Carlson, 2010)

      Expression of Gα49BdsRNA.Scer\UAS under the control of Scer\GAL4TrpA1.PR impairs thermotactic selection of 18 over 24[o]C.

      (Kwon et al., 2008)

      Expression of Galpha49BdsRNA.Scer\UAS under the control of either Scer\GAL4c309, Scer\GAL4247 (both drive expression in all lobes of the mushroom bodies) or Scer\GAL4Tab2-201Y (in the γ-lobes) leaves spontaneous odor intensity discrimination and avoidance of individual odors unchanged.

      Expression of Galpha49BdsRNA.Scer\UAS under the control of either Scer\GAL4c309, Scer\GAL4247 (both drive expression in all lobes of the mushroom bodies) or Scer\GAL4Tab2-201Y (in the γ-lobes) severely disrupts spontaneous odor identity discrimination and mildly disrupts conditioned odor identity discrimination but does not disrupt conditioned odor intensity discrimination.

      Spontaneous odor identity discrimination is significantly disrupted in transgenic Scer\GAL4c309/+; Galpha49BdsRNA.Scer\UAS, Scer\GAL80ts.αTub84B/+ flies at the restrictive (30[o]C) but not the permissive temperature.

      (Xia and Tully, 2007)

      The amplitude of light induced quantum bumps in the photoreceptors of flies expressing Gα49BdsRNA.Scer\UAS under the control of Scer\GAL4GMR.PF is reduced compared to wild type (when ATP is present in the intracellular solution during recordings).

      (Hardie et al., 2002)

      When Gα49BdsRNA.Scer\UAS is driven by Scer\GAL4Or83b.T:Hsim\VP16, olfactory behaviour is disrupted. Mutant animals appear insensitive to isoamyl acetate compared to wild-type. Response to benzaldehyde at 10-5M is normal, but at 10-4M benzaldehyde induces avoidance behaviour in mutant flies compared to attraction in controls. Responses to crushed apples or Drosophila food are normal. Mutant flies also exhibit an impaired ability to chemotax into traps containing yeast extract.

      (Kalidas and Smith, 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      GαqRNAi.UAS is rescued by Scer\GAL4Gr21a.9.323/GαqUAS.cRa

      (Yao and Carlson, 2010)
      Comments

      Co-expression of Gα49BScer\UAS.cRa restores a normal response of the ab1C olfactory receptor neuron to CO[[2]] in animals expressing Gα49BdsRNA.Scer\UAS under the control of Scer\GAL4Gr21a.9.323.

      (Yao and Carlson, 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Transcription from the P{UAS-Gα49B-dsRNA} produces Gα49B dsRNA, resulting in dsRNA interference (RNAi) of the Gα49B.

      (Kalidas and Smith, 2002)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      Gα49BdsRNA.Scer\UAS
      GαqRNAi.UAS
      GαqRNAi
      (Mishra et al., 2024)
      GαqdsRNA.Scer\UAS
      GαqdsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (7)