FlyBase Allele Report: Dmel\sra[GS3080]

General Information

Symbol

Dmel\sra^GS3080

Species

D. melanogaster

Name

FlyBase ID

FBal0138128

Feature type

allele

Associated gene

Dmel\sra

Associated Insertion(s)

P{GSV1}sra^GS3080

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Seong et al., 2001)

Progenitor genotype

Associated Insertion(s)

P{GSV1}sra^GS3080

Cytology

Description

P{GS} insertion at position +2668 (relative to the transcription start site), in an intron.

(Ejima et al., 2004)

The P{GS} insertion is in the first intron of sra, upstream of the translation start site.

(Seong et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{GSV1}

misexpression element

Regulatory region(s)

UASt

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:16,189,609..16,189,609

(Nakai et al., 2011, Takeo et al., 2006, Ejima et al., 2004, Seong et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | female, with Scer\GAL4^elav-C155

(Ejima et al., 2004)

abnormal sleep

(Nakai et al., 2011)

abnormal sleep (with sra^KO)

(Nakai et al., 2011)

female sterile | recessive

(Ejima et al., 2004)

long lived, with Scer\GAL4^hs.PB

(Seong et al., 2001)

Phenotype Manifest In

Detailed Description

Statement

Reference

sra^GS3080 mutants exhibit a decrease in in the time spent sleeping, compared to wild-type. sra^GS3080/sra^KO mutants exhibit a precipitous decrease in time spent asleep, approximately one third of the amount of wild-type sleep.

(Nakai et al., 2011)

Expression of sra^GS3080 under the control of Scer\GAL4^elav-C155 or Scer\GAL4^sca-537.4 results in increased ovulation in virgin females compared to control virgin females (the effect is stronger with Scer\GAL4^elav-C155). Within 30 minutes in a mating assay, only 20% of virgin females expressing sra^GS3080 under the control of Scer\GAL4^elav-C155 have mated, compared to 82% of wild-type virgin females. Virgin females expressing sra^GS3080 under the control of Scer\GAL4^elav-C155 elicit intermediate courtship index (CI) levels (CI is the period of time within the observation period that a male displays any element of courtship behaviour), that are between the level elicited by virgin wild-type females and mated wild-type females. Virgin females expressing sra^GS3080 under the control of Scer\GAL4^elav-C155 have a high extrusion index (EI, the percentage of a time a female shows extrusion behaviour when being courted by a male) which is nearly comparable to that of wild-type mated females. Homozygous females lay fewer eggs than wild type and most of the eggs are arrested at metaphase I of meiosis. Spontaneous ovulation occurs in 3% of mutant virgin females (virtually no ovulation occurs in wild-type virgin females). The level of ovulation of mutant mated females is not as high as that of wild-type mated females. The mutant females show reduced receptivity after mating (as occurs in wild-type females), but their remating rate 24 hours after mating is significantly higher than that of wild-type females. Receptivity of mutant females is restored earlier than for wild-type females; the remating rate is significantly higher for mutant females compared to wild-type females 10 days after mating.

(Ejima et al., 2004)

Mutant animals have a mean longevity of about 32.6 days at 30^oC.

(Seong et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

sra^GS3080/sra^KO has abnormal sleep phenotype, suppressible | partially by CanA-14F^KO

(Nakai et al., 2011)

sra^GS3080/sra^KO has abnormal sleep phenotype, suppressible | partially by Pp2B-14D^KO

(Nakai et al., 2011)

Enhancer of

Statement

Reference

sra^GS3080/sra^KO is an enhancer of abnormal sleep phenotype of CanA-14F^KO

(Nakai et al., 2011)

sra^GS3080/sra^KO is an enhancer of abnormal sleep phenotype of Pp2B-14D^KO

(Nakai et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

A CanA-14F^KO background partially suppresses the reduction in total sleep found in sra^GS3080/sra^KO mutants.

A Pp2B-14D^KO background partially suppresses the reduction in total sleep found in sra^GS3080/sra^KO mutants.

(Nakai et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

205062

y¹ w^67c23; P{GSV1}sra^GS3080/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

Comments

Excision of the P{GS} element can restore female fertility.

(Ejima et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

sra^GS3080

(Nakai et al., 2011, Takeo et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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