FB2024_03 , released June 25, 2024
Allele: Dmel\Sac1L2F
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General Information
Symbol
Dmel\Sac1L2F
Species
D. melanogaster
Name
FlyBase ID
FBal0141545
Feature type
allele
Associated gene
Dmel\Sac1
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Wei et al., 2003, Wei et al., 2003)
Progenitor genotype
Cytology
Description

Amino acid replacement: W438term.

(Wei et al., 2003, Wei et al., 2003)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:1,249,603..1,249,603 [+]
Nucleotide change:

G1249603A

Amino acid change:

W438term | Sac1-PA; W438term | Sac1-PB

Reported amino acid change:

W438term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Wei et al., 2003)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous embryos have dorsal anterior holes in the cuticle. They also show puckering of the dorsal cuticle and an absence of dorsal hairs along the midline. Homozygous and Sac1L2F/Df(3L)Fpa2 embryos show apical constriction of the middle amnioserosa cells at the onset of dorsal closure (in wild-type embryos, the middle amnioserosa cells are the last to show apical constriction during dorsal closure). Sac1BG02228/Sac1L2F and Sac1L2F/Df(3L)B67 embryos have dorsal anterior holes in the cuticle and puckering of the dorsal cuticle. Heterozygous embryos do not show cuticle defects.

      (Wei et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      Sac1[+]/Sac1L2F is a suppressor of embryonic/first instar larval cuticle | dorsal phenotype of bsk1

      (Wei et al., 2003)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The frequency of dorsal holes in the cuticle seen in bsk1 embryos is partially suppressed by Sac1L2F/+.

      (Wei et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Sac1L2F is rescued by Sac1+t2.8

      (Wei et al., 2003)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Sac1L2F
      dsac1L2F
      (Wei et al., 2003)
      l(3)2107L2F
      (Wei et al., 2003)
      sac1L2F
      (Wei et al., 2003)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)