FB2024_03 , released June 25, 2024
Gene: Dmel\Sac1
Open Close
General Information
Symbol
Dmel\Sac1
Species
D. melanogaster
Name
Sac1 phosphatase
Annotation Symbol
CG9128
Feature Type
protein_coding_gene
FlyBase ID
FBgn0283500
Gene Model Status
Current
Stock Availability
12 publicly available
Gene Summary
Sac1 phosphatase (Sac1) encodes a lipid phosphatase that dephosphorylates phosphatidylinositol 4-phosphate to generate phosphatidylinositol. It is involved in dorsal closure, Hedgehog signaling and axon guidance. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Also Known As

impatent

Key Links
Genomic Location
Cytogenetic map
61F4-61F4
Sequence location
Recombination map
3-0
RefSeq locus
NT_037436 REGION:1247817..1250451
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (19 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables phosphatidylinositol phosphate phosphatase activity
inferred from direct assay
(Lee et al., 2011)
enables phosphatidylinositol-4-phosphate phosphatase activity
inferred from mutant phenotype
(Yavari et al., 2010)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables phosphatase activity
inferred from sequence or structural similarity with UniProtKB:Q9ES21
(Gene Ontology Curators, 2002-)
inferred from electronic annotation with InterPro:IPR002013
(InterPro Project Members, 2004-)
enables phosphatidylinositol-4-phosphate phosphatase activity
inferred from biological aspect of ancestor with PANTHER:PTN000127780
(GO Reference Genome Project, 2011-)
Biological Process (10 terms)
Terms Based on Experimental Evidence (10 terms)
CV Term
Evidence
References
involved_in axo-dendritic transport
inferred from mutant phenotype
(Forrest et al., 2013)
involved_in axon midline choice point recognition
inferred from mutant phenotype
(Lee et al., 2011)
involved_in cytoplasmic microtubule organization
inferred from mutant phenotype
(Forrest et al., 2013)
involved_in dorsal closure, amnioserosa morphology change
inferred from mutant phenotype
(Wei et al., 2003)
involved_in lumen formation, open tracheal system
inferred from high throughput mutant phenotype
(Ghabrial et al., 2011)
involved_in negative regulation of JNK cascade
inferred from mutant phenotype
(Wei et al., 2003)
inferred from genetic interaction with FLYBASE:puc; FB:FBgn0243512
(Wei et al., 2003)
involved_in negative regulation of smoothened signaling pathway
inferred from mutant phenotype
(Yavari et al., 2010)
involved_in phosphatidylinositol dephosphorylation
inferred from mutant phenotype
(Yavari et al., 2010)
involved_in phosphatidylinositol metabolic process
inferred from mutant phenotype
(Forrest et al., 2013)
involved_in synapse maturation
inferred from mutant phenotype
(Forrest et al., 2013)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in phosphatidylinositol dephosphorylation
inferred from biological aspect of ancestor with PANTHER:PTN000127780
(GO Reference Genome Project, 2011-)
Cellular Component (6 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
located_in axon
inferred from direct assay
(Lee et al., 2011)
located_in endomembrane system
inferred from high throughput direct assay
(Tan et al., 2009)
located_in neuronal cell body
inferred from direct assay
(Lee et al., 2011)
located_in sensory dendrite
inferred from direct assay
(Lee et al., 2011)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
located_in endoplasmic reticulum membrane
inferred from sequence or structural similarity with UniProtKB:Q9ES21
(Gene Ontology Curators, 2002-)
is_active_in endoplasmic reticulum
inferred from biological aspect of ancestor with PANTHER:PTN000127780
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
phosphatidylinositol-4-phosphate phosphatase activity
RHEA 55652:
Summaries
Gene Snapshot
Sac1 phosphatase (Sac1) encodes a lipid phosphatase that dephosphorylates phosphatidylinositol 4-phosphate to generate phosphatidylinositol. It is involved in dorsal closure, Hedgehog signaling and axon guidance. [Date last reviewed: 2019-03-14]
Gene Group (FlyBase)
PHOSPHATIDYLINOSITOL PHOSPHATE AND INOSITOL PHOSPHATE PHOSPHATASES -
Phosphoinositide phosphate (PtdInsP) phosphatases and Inositol phosphate (InsP) phosphatases are enzymes that catalyze the hydrolysis of phosphate groups from inositol. Many of these phosphatases can act on both PtdInsP and InsP substrates. (Adapted from PMID:23681533).
Pathway (FlyBase)
Negative Regulators of Hedgehog Signaling Pathway -
Negative regulators of hedgehog signaling down-regulate the pathway, resulting in the repression of transcription of hh-responsive genes.
Protein Function (UniProtKB)
Phosphoinositide phosphatase which catalyzes the hydrolysis of phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 4-phosphate (PtdIns(4)P) (By similarity). Has low activity towards phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2) (By similarity).
(UniProt, Q9W0I6)
Summary (Interactive Fly)

PI4P phosphatase, lipid phosphatase - promotes microtubule stability in the developing retina, regulates postembryonic synaptic maturation and neurodegeneration - binding partner of VAMP-associated protein 33kDa (Vap33 or DVap) - Drosophila model of amyotrophic lateral sclerosis, functions downstream of STT4 and Ptc in the regulation of Smo membrane localization and Hh pathway activation - dorsal closure - axonal transport - microtubule stability

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Sac1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9W0I6)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene
gene
(Ghabrial et al., 2011)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Sac1-RA
FBtr0072652
NM_138243
2365
592
Sac1-RB
FBtr0303217
NM_001202087
2327
592
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Sac1-PA
FBpp0072546
67.8
592
8.45
Q9W0I6
NP_612087
AAF47460
Sac1-PB
FBpp0292309
67.8
592
8.45
Q9W0I6
NP_001189016
ADV37453
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

592 aa isoforms: Sac1-PA, Sac1-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Sac1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

3.76

Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 3
primordial germ cell
(Lee et al., 2011)
organism | ubiquitous
(Lee et al., 2011)
embryonic stage 13
embryonic dorsal epidermis
(Lee et al., 2011)
amnioserosa | surrounding
(Lee et al., 2011)
embryonic stage 16
sensory neuron
(Lee et al., 2011)
larval longitudinal connective | subset
(Lee et al., 2011)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
Additional Descriptive Data

Sac1 is highly expressed during early stages of embryogenesis and is most prominent in the pole cells. At embryonic stage 13 and 16, expression is mostly restricted to the developing central and peripheral nervous systems. Expression is also observed in the dorsal epidermis around the amnioserosa at stage 13. Sac1 expression at stage 16 completely overlaps the 22C10 antibody pattern in the PNS showing strong expression in all sensory neurons. In the CNS, Sac1 is expressed in a subset of longitudinal fascicles.

(Lee et al., 2011)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in axon
inferred from direct assay
(Lee et al., 2011)
located_in endomembrane system
inferred from high throughput direct assay
(Tan et al., 2009)
located_in neuronal cell body
inferred from direct assay
(Lee et al., 2011)
located_in sensory dendrite
inferred from direct assay
(Lee et al., 2011)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Sac1 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 14 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 14 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Sac1
Transgenic constructs containing regulatory region of Sac1
Aberrations (Deficiencies and Duplications) ( 6 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal (with Df(3L)3C7)
lethal (with Df(3L)bab-PG)
lethal | pupal stage, with Scer\GAL4pnr-MD237
lethal - all die before end of larval stage
lethal - all die during embryonic stage | recessive
partially lethal, with Scer\GAL4elav.PU
partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
abnormal neuroanatomy | embryonic stage (with Sac11)
abnormal neuroanatomy | embryonic stage (with Sac12)
abnormal neuroanatomy | embryonic stage (with Sac12107)
abnormal neuroanatomy | larval stage, with Scer\GAL4C57
abnormal neuroanatomy | larval stage, with Scer\GAL4elav.PU
increased cell death, with Scer\GAL4elav.PU
short lived, with Scer\GAL4Mef2.PR
some die during embryonic stage | recessive
some die during larval stage
visible, with Scer\GAL4elav.PU
visible (with Df(3L)3C7)
visible (with Sac1L4H)
visible (with Sac1L16C)
Phenotype manifest in
Allele
amnioserosa
amnioserosa (with Df(3L)Fpa2)
embryonic/first instar larval cuticle | dorsal
embryonic/first instar larval cuticle | dorsal (with Df(3L)B67)
embryonic/first instar larval cuticle | dorsal (with Df(3L)Fpa2)
embryonic/first instar larval cuticle | dorsal (with Sac1BG02228)
embryonic/first instar larval cuticle | dorsal (with Sac1L2F)
embryonic/larval tracheal system
eye, with Scer\GAL4elav.PU
eye (with Df(3L)3C7)
eye (with Sac1L4H)
eye (with Sac1L16C)
eye | somatic clone
interommatidial bristle, with Scer\GAL4elav.PU
larval dorsal hair
NMJ bouton, with Scer\GAL4elav.PU
NMJ bouton | larval stage, with Scer\GAL4C57
ommatidium, with Scer\GAL4elav.PU
terminal tracheal cell
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (6)
Hsap\SACM1L
14 of 14
Yes
Yes
Hsap\FIG4
3 of 14
No
No
Hsap\INPP5F
2 of 14
No
No
Hsap\SYNJ1
2 of 14
No
No
Hsap\SYNJ2
2 of 14
No
No
Hsap\INPP5B
1 of 14
No
No
1  
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (6)
Rnor\Sacm1l
14 of 14
Yes
Yes
Rnor\Fig4
3 of 14
No
No
Rnor\Inpp5f
3 of 14
No
No
Rnor\Synj1
2 of 14
No
No
Rnor\Synj2
2 of 14
No
No
Rnor\Inpp5b
1 of 14
No
No
Mus musculus (laboratory mouse) (5)
Mmus\Sacm1l
14 of 14
Yes
Yes
Mmus\Fig4
3 of 14
No
No
Mmus\Inpp5f
2 of 14
No
No
Mmus\Synj1
2 of 14
No
No
Mmus\Synj2
2 of 14
No
No
Xenopus tropicalis (Western clawed frog) (5)
Xtro\sacm1l
6 of 13
Yes
Yes
Xtro\synj1
2 of 13
No
No
Xtro\synj2
2 of 13
No
No
Xtro\fig4
1 of 13
No
No
Xtro\inpp5f
1 of 13
No
No
Danio rerio (Zebrafish) (8)
Drer\sacm1la
14 of 14
Yes
Yes
Drer\sacm1lb
14 of 14
Yes
Yes
Drer\fig4a
2 of 14
No
No
Drer\inpp5f
2 of 14
No
No
Drer\synj1
2 of 14
No
No
Drer\fig4b
1 of 14
No
Yes
Drer\inpp5b
1 of 14
No
No
Drer\inppl1b
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (4)
Cele\sac-1
14 of 14
Yes
Yes
Cele\figo-1
3 of 14
No
No
Cele\sac-2
2 of 14
No
No
Cele\unc-26
2 of 14
No
No
Anopheles gambiae (African malaria mosquito) (4)
Agam\AgaP_AGAP000891
12 of 12
Yes
Yes
Agam\AgaP_AGAP004477
3 of 12
No
No
Agam\AgaP_AGAP000483
2 of 12
No
No
Agam\AgaP_AGAP012175
2 of 12
No
No
Arabidopsis thaliana (thale-cress) (9)
Atha\ATSAC1B
13 of 13
Yes
Yes
Atha\RHD4
13 of 13
Yes
Yes
Atha\SAC8
11 of 13
No
Yes
Atha\AT1G17340
3 of 13
No
No
Atha\AT3G14205
3 of 13
No
No
Atha\AT3G43220
3 of 13
No
No
Atha\AT5G20840
3 of 13
No
No
Atha\SAC9
3 of 13
No
Yes
Atha\ATSAC1
2 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (5)
Scer\SAC1
13 of 13
Yes
Yes
Scer\FIG4
2 of 13
No
No
Scer\INP51
1 of 13
No
No
Scer\INP52
1 of 13
No
No
Scer\INP53
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (6)
Spom\SPBC19F5.03
12 of 12
Yes
Yes
Spom\SPAC3C7.01c
6 of 12
No
Yes
Spom\SPAC1093.03
2 of 12
No
No
Spom\SPAC9G1.10c
1 of 12
No
No
Spom\syj1
1 of 12
No
No
Spom\syj2
1 of 12
No
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Sac1. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (7)
FIG4
4 of 13
sp3
4 of 13
Synj
3 of 13
CG6805
1 of 13
CG9784
1 of 13
INPP5E
1 of 13
Ocrl
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    Sac1BG02228
    exacerbates  autosomal dominant cerebellar ataxia
    modeled by Hsap\ATXN8OSCTG112.UAS
    (Mutsuddi et al., 2004)
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    SACM1L; SAC1 like phosphatidylinositide phosphatase
    14 of 14
    606569
        FIG4; FIG4 phosphoinositide 5-phosphatase
        3 of 14
        609390
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Show/hide secondary interactors 
        (data from AllianceMine provided by esyN)
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Other Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        Sac1 - Rab23
        anti tag coimmunoprecipitation, peptide massfingerprinting
        (Çiçek et al., 2016)
        Sac1 - Vap33
        two hybrid, anti tag coimmunoprecipitation, anti tag western blot
        (Forrest et al., 2013)
        Summary of Genetic Interactions
        esyN Network Diagram
        Show/hide secondary interactors 
        (data from AllianceMine provided by esyN)
        esyN Network Key:
        Suppression
        Enhancement
        Other Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Sac1
        suppressible
        crb
        (Wei et al., 2003)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        bsk
        suppressible
        Sac1
        (Wei et al., 2003)
        External Data
        Linkouts
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Negative Regulators of Hedgehog Signaling Pathway -
        Negative regulators of hedgehog signaling down-regulate the pathway, resulting in the repression of transcription of hh-responsive genes.
        Metabolic Pathways
        KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
        External Data
        Linkouts
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3L
        Recombination map
        3-0
        Cytogenetic map
        61F4-61F4
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        61F4-61F4
        Limits computationally determined from genome sequence between P{PZ}l(3)0596705967 and P{PZ}l(3)0264002640
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Location based on deficiency mapping with Df(3L)bab-PG.
        (Ghabrial et al., 2011)
        61-61
        (Ghabrial et al., 2011)
        Experimentally Determined Recombination Data
        Location

        3-0

        (FlyBase, 2016)

        3-1.5

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (12)
        Genomic Clones (18)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (128)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        Antibody Information
        Laboratory Generated Antibodies
         

        polyclonal

        (Lee et al., 2011)
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments

            A weak Sac1 allele causes defects in eye development, while stronger alleles die as embryos with defects in head involution and dorsal closure.

            (Wei et al., 2003)

            Sac1 contributes to negative regulation of the JNK cascade during dorsal closure.

            (Wei et al., 2003)

            Mutants die as embryos with defects in dorsal closure.

            (Wei et al., 2003)
            Relationship to Other Genes
            Source for database merge of

            Source for merge of: Sac1 impatent

            (Ghabrial, 2015.8.26)
            Additional comments
            Nomenclature History
            Source for database identify of

            Source for identity of: Sac1 CG9128

            (Wei, 2003.2.12)
            Nomenclature comments
            Etymology
            Synonyms and Secondary IDs (8)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 43 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
            References (60)