Insertion of P{PdL} into 5'UTR of VhaSFD, in the same orientation as VhaSFD transcription.
long lived, with Tn10\tetRrtTA.Act5C
When expression is driven by Tn10\tetRAct5C.T:Hsim\VP16 from P{rtTA.B}E2 lifespan is increased on average by 8%.