FlyBase Allele Report: Dmel\park[13]

General Information

Symbol

Dmel\park¹³

Species

D. melanogaster

Name

FlyBase ID

FBal0146939

Feature type

allele

Associated gene

Dmel\park

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Greene et al., 2003)

Mutagen

P-element activity

(Greene et al., 2003)

Progenitor genotype

park^EPLA1

(Greene et al., 2003)

Cytology

Description

The entire park gene has been deleted.

(Greene et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease 2

CEC

(Das et al., 2023)

CEA

(Dwivedi and Lakhotia, 2016)

model of Parkinson's disease

CEA

(Greene et al., 2003)

model of cardiomyopathy

CEA

(Bhandari et al., 2014)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Parkinson's disease 2

is ameliorated by spoon^KG02745

(Das et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate (with Df(3L)Pc-MK)

(Greene et al., 2003)

abnormal flight (with Df(3L)Pc-MK)

(Greene et al., 2003)

abnormal locomotor behavior (with Df(3L)Pc-MK)

(Greene et al., 2003)

decreased rate of adult locomotory behavior | dominant | progressive

(Das et al., 2023)

female fertile (with Df(3L)Pc-MK)

(Greene et al., 2003)

male sterile (with Df(3L)Pc-MK)

(Greene et al., 2003)

short lived

(Bhandari et al., 2014)

short lived (with Df(3L)Pc-MK)

(Greene et al., 2003)

short lived (with park^Δ21)

(Tan et al., 2018)

viable (with park^Δ21)

(Tan et al., 2018)

Phenotype Manifest In

adult heart

(Bhandari et al., 2014)

adult heart chamber

(Chen and Dorn, 2013)

cardial cell

(Bhandari et al., 2014, Chen and Dorn, 2013)

dopaminergic neuron (with Df(3L)Pc-MK)

(Greene et al., 2003)

embryonic/larval hypodermal muscle cell | third instar larval stage (with park^Δ21)

(Tan et al., 2018)

mitochondrion

(Bhandari et al., 2014, Chen and Dorn, 2013)

mitochondrion | third instar larval stage (with park^Δ21)

(Tan et al., 2018)

Nebenkern (with Df(3L)Pc-MK)

(Greene et al., 2003)

nucleus | third instar larval stage (with park^Δ21)

(Tan et al., 2018)

Detailed Description

Statement

Reference

Body wall muscles in park^Δ21/park¹³ transheterozygous third instar larvae frequently show nuclei clustering together or in contact with each other, instead of the typically even spaced in controls, and show larger and more globular mitochondria. Nuclei clustering is not observed in the park¹³ heterozygous background.

(Tan et al., 2018)

At one and four weeks after eclosure, heart tubes of park¹³ mutant flies exhibit reduced fractional shortening compared to controls. Ultrastructural examination of park¹³ heart tubes reveals abnormally enlarged cardiomyocyte mitochondria, many of which have disorganised cristae or a characteristic hollow donut morphology. Loss of cardiomyocyte mitochondrial nucleoids is also seen, as well as an increased number of depolarized cardiomyocyte mitochondria and greater numbers of mitochondrion producing reactive oxygen species (ROS).

(Bhandari et al., 2014)

Cardiomyocyte mitochondria from park¹³ animals are enlarged, and heart tubes exhibit impaired respiration with chamber dilation and contractile impairment.

(Chen and Dorn, 2013)

park¹³/Df(3L)Pc-MK animals eclose a day later than wild-type and have an average lifespan of 27 days (as opposed to 39 days for wild-type). park¹³/Df(3L)Pc-MK males are sterile. Spermatogenesis appears to proceed normally in mutants until the individualisation stage, at which point a 64-cell germ-line cyst that normally separates into mature sperm cells fails to do so, resulting in an absence of mature sperm in the seminal vesicle. The axonemes in mutant males appear normal, but Nebenkern integrity is severely disrupted; some spermatids have multiple Nebenkern, whereas others have only an extremely diminished component. park¹³/Df(3L)Pc-MK animals exhibit impaired flight and climbing ability compared to controls. park¹³/Df(3L)Pc-MK animals do not exhibit a general neuronal degeneration, and no age-related increase in neurodegeneration is evident. In the dorsomedial cluster, the number of neurons appear normal though shrinkage of the cell body is seen.

(Greene et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

park¹³ has decreased rate of adult locomotory behavior | dominant | progressive phenotype, suppressible | partially by spoon^KG02745

(Das et al., 2023)

park^Δ21/park¹³ has short lived phenotype, suppressible | partially by Hsap\ARIH1^UAS.cFa/Scer\GAL4^da.G32