FlyBase Allele Report: Dmel\Sirt1[17]

General Information

Symbol

Dmel\Sirt1¹⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0147418

Feature type

allele

Associated gene

Dmel\Sirt1

Associated Insertion(s)

Carried in Construct

Also Known As

sir2¹⁷, dSir2¹⁷

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Astrom et al., 2003)

Progenitor genotype

P{lacW}k07223

(Astrom et al., 2003)

Cytology

Description

Imprecise excision of the P{lacW} element, removing most of the Sir2 open reading frame (sequences coding for the first 579 amino acids are missing).

(Astrom et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Smith et al., 2014)

exacerbates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Smith et al., 2014)

ameliorates neurodegenerative disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Pallos et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Joyce and McKim, 2010)

suppressor of variegation, with In(1)w^m4

(Astrom et al., 2003)

viable | female

(Astrom et al., 2003)

viable | female (with Sirt1^ex10)

(Astrom et al., 2003)

viable | male

(Astrom et al., 2003)

viable | male (with Sirt1^ex10)

(Astrom et al., 2003)

Phenotype Manifest In

Detailed Description

Statement

Reference

The median lifespan of Sir2¹⁷ flies (mutant line generated by outcrossing the mutation into the Canton S background) responds in the same way as that of Canton S controls to a range of diets (from dietary restriction to fully-fed conditions).

(Burnett et al., 2011)

Sir2¹⁷ heterozygotes do not display abnormal longevity.

(Pallos et al., 2008)

Heterozygous males do not have ectopic sex combs on the T2 or T3 legs.

(Furuyama et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Sirt1¹⁷ is an enhancer of abnormal neuroanatomy phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Smith et al., 2014)

Sirt1¹⁷ is an enhancer of abnormal locomotor behavior phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Smith et al., 2014)

Sirt1¹⁷/Sir2[+] is an enhancer of visible | dominant | homeotic phenotype of Pcl¹¹

(Furuyama et al., 2004)

Sirt1¹⁷/Sir2[+] is an enhancer of visible | dominant | homeotic phenotype of Pc³

(Furuyama et al., 2004)

Suppressor of

Statement

Reference

Sirt1¹⁷/Sir2[+] is a suppressor of abnormal neuroanatomy phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Smith et al., 2014, Pallos et al., 2008)

Sirt1¹⁷/Sir2[+] is a suppressor of abnormal locomotor behavior phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Smith et al., 2014)

Sirt1¹⁷/Sir2[+] is a suppressor of increased mortality phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Pallos et al., 2008)

Sirt1¹⁷/Sir2[+] is a suppressor of long lived phenotype of HDAC1^def24

(Rogina and Helfand, 2004)

Phenotype Manifest In

Enhancer of

Statement

Reference

Sirt1¹⁷ is an enhancer of retina phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Smith et al., 2014)

Sirt1¹⁷/Sir2[+] is an enhancer of mesothoracic leg phenotype of Pcl¹¹

(Furuyama et al., 2004)

Sirt1¹⁷/Sir2[+] is an enhancer of sex comb | ectopic phenotype of Pcl¹¹

(Furuyama et al., 2004)

Sirt1¹⁷/Sir2[+] is an enhancer of mesothoracic leg phenotype of Pc³

(Furuyama et al., 2004)

Sirt1¹⁷/Sir2[+] is an enhancer of sex comb | ectopic phenotype of Pc³

(Furuyama et al., 2004)

Suppressor of

Statement

Reference

Sirt1¹⁷/Sir2[+] is a suppressor of retina phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Smith et al., 2014)

Sirt1¹⁷/Sir2[+] is a suppressor of germarium region 3 phenotype of polo^S025604/polo¹

(Pek et al., 2012)

Sirt1¹⁷/Df(2L)BSC245 is a suppressor of stage S1 oocyte | increased number phenotype of hdm^g7

(Joyce and McKim, 2010)

Sirt1¹⁷/Df(2L)BSC245 is a suppressor of stage S1 oocyte | increased number phenotype of Scer\GAL4^{VP16.nanos.UTR}, pch2^UASp.Tag:HA

(Joyce and McKim, 2010)

Sirt1¹⁷/Df(2L)BSC245 is a suppressor of stage S1 oocyte | increased number phenotype of spn-B^BU

(Joyce and McKim, 2010)

Sirt1¹⁷/Sir2[+] is a suppressor of photoreceptor neuron phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Pallos et al., 2008)

Sirt1¹⁷/Sir2[+] is a suppressor of rhabdomere phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Pallos et al., 2008)

Other

Statement

Reference

Pc³, Sirt1¹⁷/Sir2[+] has metathoracic leg phenotype

(Furuyama et al., 2004)

Pcl¹¹, Sirt1¹⁷/Sir2[+] has metathoracic leg phenotype

(Furuyama et al., 2004)

Pc³, Sirt1¹⁷ has metathoracic leg phenotype

(Furuyama et al., 2004)

Pcl¹¹, Sirt1¹⁷ has metathoracic leg phenotype

(Furuyama et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Sir2¹⁷/+ suppresses the defect in oocyte commitment (high frequency of 'two-oocyte phenotype' in region 3 of the germarium) that is seen in polo¹/polo^S025604 germaria.

(Pek et al., 2012)

The frequency of ectopic sex combs seen in Pc³/+ males is increased by Sir2¹⁷; in Sir2¹⁷/+ ; Pc³/+ double heterozygous males, 94% of T2 legs and 63% of T3 legs have sex combs. The frequency of ectopic sex combs seen in Pcl¹¹/+ males is increased by Sir2¹⁷; in Sir2¹⁷/+ ; Pcl¹¹/+ double heterozygous males, 71% of T2 legs and 38% of T3 legs have sex combs. Eye colour: Sir2¹⁷/+ moderately, though consistently, impairs the silencing of the heterozygous w^UbxPRE.2.2 transgene, resulting in a slight increase in eye pigmentation.

(Furuyama et al., 2004)

The increased average lifespan of Rpd3^def24 homozygous flies is suppressed by Sir2¹⁷/+.

(Rogina and Helfand, 2004)

Xenogenetic Interactions

Statement

Reference

When Scer\GAL4^elav-C155>Hsap\HTT^{Q93.ex1p.Scer\UAS}-expressing animals are also heterozygous for Sir2¹⁷, the extent of neuronal loss is reduced and show improved motor function compared with siblings wild type Sir2. However, homozygous Sir2¹⁷ enhances the neuronal loss phenotype and exacerbates the defects in motor function.

(Smith et al., 2014)

A heterozygous Sir2¹⁷ background markedly increases the survival rate of flies neuronally-expressing Hsap\HTT^{Q93.ex1p.Scer\UAS} flies (from <10% to nearer 35% survival to eclosion). In addition, the level of neurodegeneration, as measured by the ratio of rhabdomeres per ommatidia, is also lowered in a Sir2¹⁷ heterozygous background.

(Pallos et al., 2008)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

24857

w¹¹¹⁸; Sirt1¹⁷/SM6a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Sir2¹⁷

(Smith et al., 2014, Debruhl et al., 2013, Pallos et al., 2008, Wood et al., 2004)

Sirt1¹⁷

(Faragó et al., 2022)

dSir2¹⁷

(Burnett et al., 2011, Rogina and Helfand, 2004)

dsir2¹⁷

(Astrom et al., 2003)

sir2¹⁷

(Pek et al., 2012, Joyce and McKim, 2010)

Name Synonyms

Secondary FlyBase IDs

References (12)

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