FlyBase Allele Report: Dmel\Diap1[UASp.cPa]

General Information

Symbol

Dmel\Diap1^UASp.cPa

Species

D. melanogaster

Name

FlyBase ID

FBal0150217

Feature type

allele

Associated gene

Dmel\Diap1

Associated Insertion(s)

Carried in Construct

P{UASp-Diap1.P}

Also Known As

UASp-DIAP1, UAS-diap1

Key Links

Transgenic product class

cDNA

(Peterson et al., 2003)

wild_type

(Peterson et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Peterson et al., 2003)

wild_type

(Peterson et al., 2003)

Mutagen

in vitro construct

(Peterson et al., 2003)

Progenitor genotype

Carried in construct

P{UASp-Diap1.P}

Cytology

Description

UASp regulatory sequences control expression of Diap1 (+421 to +1748 of Diap1 cDNA (GenBank: L49440)).

(Peterson et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Diap1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates carcinoma

modeled by Egfr^UAS.cBa, Socs36E^GD8929

(Xu et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^nanos.PG

(Baum et al., 2007)

abnormal cell death | adult stage | nutrition conditional

(Etchegaray et al., 2012)

Phenotype Manifest In

egg chamber, with Scer\GAL4^{VP16.nanos.UTR}

(Mazzalupo and Cooley, 2006)

egg chamber, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^nanos.PG

(Baum et al., 2007)

egg chamber | nutrition conditional

(Etchegaray et al., 2012)

follicle cell | nutrition conditional

(Etchegaray et al., 2012)

Detailed Description

Statement

Reference

Engulfment of nurse cells by follicle cells is largely inhibited in the "undead" egg chambers (egg chambers in which the nurse cell nuclei fail to condense and the follicle cells disappear) of starved flies expressing th^{Scer\UAS.P\T.cPa} in the germline under the control of Scer\GAL4^nos.PG. Most of the follicle cells fail to enlarge and display thinning out of the membranes. 36% of undead egg chambers show some FC enlargement.

(Etchegaray et al., 2012)

Expression of th^{Scer\UAS.P\T.cPa} under the control of both Scer\GAL4^nos.PG and Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in defective egg chambers during mid-oogenesis (stages 7-9), characterised by a lack of follicle cells and the presence of large, uncondensed nurse cell nuclei. Approximately 20% of stage 14 egg chambers show uncondensed, persisting nurse cell nuclei.

(Baum et al., 2007)

The base of the ovaries of starved females expressing th^{Scer\UAS.P\T.cPa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} is filled with a mass of unhealthy, undead egg chamber tissue, including large uncondensed nurse cell nuclei. Residual egg chambers are devoid of follicle cell nuclei.

(Mazzalupo and Cooley, 2006)

Normal nurse cell death occurs in th^{Scer\UAS.P\T.cPa}; Scer\GAL4^{nos.UTR.T:Hsim\VP16}/Scer\GAL4^{nos.UTR.T:Hsim\VP16} adult females.

(Peterson et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Diap1^UASp.cPa, Scer\GAL4^{VP16.nanos.UTR} is a suppressor of sterile phenotype of Dcp-1^ΔN.UASp, Scer\GAL4^{VP16.nanos.UTR}

(Peterson et al., 2003)

NOT Suppressor of

Statement

Reference

Diap1^UASp.cPa, Scer\GAL4^{VP16.mat.αTub67C} is a non-suppressor of increased cell death phenotype of Scer\GAL4^{VP16.mat.αTub67C}, lok^UASp.Tag:HA

(Bakhrat et al., 2010)

Diap1^UASp.cPa, Scer\GAL4^{VP16.nanos.UTR} is a non-suppressor of increased cell death | germline clone phenotype of Scer\GAL4^{VP16.nanos.UTR}, p53^UASp.cBa

(Bakhrat et al., 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

Diap1^UASp.cPa, Scer\GAL4^{VP16.nanos.UTR} is a suppressor | partially of egg chamber phenotype of Dcp-1^ΔN.UASp, Scer\GAL4^{VP16.nanos.UTR}

(Peterson et al., 2003)

NOT Suppressor of

Statement

Reference

Diap1^UASp.cPa, Scer\GAL4^{VP16.mat.αTub67C} is a non-suppressor of egg chamber | oogenesis stage S8 phenotype of Scer\GAL4^{VP16.mat.αTub67C}, lok^UASp.Tag:HA

(Bakhrat et al., 2010)

Diap1^UASp.cPa, Scer\GAL4^{VP16.mat.αTub67C} is a non-suppressor of egg chamber | oogenesis stage S9 phenotype of Scer\GAL4^{VP16.mat.αTub67C}, lok^UASp.Tag:HA

(Bakhrat et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Overexpression of th^{Scer\UAS.P\T.cPa} with p53^{Scer\UAS.P\T.cBa}, during oogenesis under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not suppress the p53^{Scer\UAS.P\T.cBa}-dependent stem cell loss.

(Bakhrat et al., 2010)

th^{Scer\UAS.P\T.cPa} can partially suppress the sterility and egg chamber degeneration seen in adult females carrying one copy of P{UASp-Dcp-1.ΔN} and two copies of P{GAL4::VP16-nos.UTR} raised at 25^oC. However, the resulting egg chambers sometimes lack follicle cells and/or have extra nurse cells.

(Peterson et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

63818