FlyBase Allele Report: Dmel\loj[00898]

General Information

Symbol

Dmel\loj⁰⁰⁸⁹⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0150354

Feature type

allele

Associated gene

Dmel\loj

Associated Insertion(s)

P{PZ}loj⁰⁰⁸⁹⁸

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Carney and Taylor, 2003)

Progenitor genotype

Associated Insertion(s)

P{PZ}loj⁰⁰⁸⁹⁸

Cytology

Description

P{PZ} insertion in the 5' untranslated region of the loj class I, Ia and III transcripts.

(Carney and Taylor, 2003)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile

(Boltz et al., 2007)

female sterile (with loj⁰⁴⁰²⁶)

(Saleem et al., 2012, Boltz et al., 2007)

female sterile | recessive

(Saleem et al., 2012, Carney and Taylor, 2003)

viable (with loj⁰⁴⁰²⁶)

(Carney and Taylor, 2003)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous loj⁰⁰⁸⁹⁸ mutant females that have been mated with wild type males are unable to lay eggs. However they are able to ovulate and move eggs through the uterus, as 85% of flies have an egg lodged in their uterus and the remainder have an egg in the oviduct. loj⁰⁰⁸⁹⁸ heterozygotes are able to lay eggs and only 35% of females have an egg in the uterus upon dissection.

The oviposition defects seen in loj⁰⁰⁸⁹⁸/loj⁰⁴⁰²⁶ mutant females cannot be rescued through feeding the flies either octopamine or glutamate.

(Saleem et al., 2012)

loj⁰⁰⁸⁹⁸ homozygotes or loj⁰⁰⁸⁹⁸/loj⁰⁴⁰²⁶ mutants show oviposition defects rendering them sterile.

(Boltz et al., 2007)

In homozygous and loj⁰⁰⁸⁹⁸/Df(3L)ZN47 females, one or more mature eggs become lodged within the genital tract, causing a "logjam" of eggs within the female and preventing the release of eggs. In almost all cases an egg is found in the uterus and in many females eggs are also found within the lateral or common oviducts. Homozygous and loj⁰⁰⁸⁹⁸/Df(3L)ZN47 females mate with latency and frequency similar to that of control females when mated to wild-type males.

(Carney and Taylor, 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

loj⁰⁴⁰²⁶

(Carney and Taylor, 2003)

Rescued by

loj⁰⁰⁸⁹⁸/loj⁰⁴⁰²⁶ is rescued by Scer\GAL4^ptc-559.1/loj^UAS.N9d

(Saleem et al., 2012)

loj⁰⁰⁸⁹⁸/loj⁰⁴⁰²⁶ is rescued by loj^UAS.N9d/Scer\GAL4^C164

(Saleem et al., 2012)

loj⁰⁰⁸⁹⁸/loj⁰⁴⁰²⁶ is rescued by loj^UAS.N9d/Scer\GAL4^dimm-929

(Saleem et al., 2012)

loj⁰⁰⁸⁹⁸ is rescued by Scer\GAL4^CY2/loj^UAS.N9d

(Boltz et al., 2007)

loj⁰⁰⁸⁹⁸ is rescued by loj^UAS.N9d/Scer\GAL4^SG18.1

(Boltz et al., 2007)

loj⁰⁰⁸⁹⁸/loj⁰⁴⁰²⁶ is rescued by loj^UAS.N9d/Scer\GAL4^SG18.1

(Boltz et al., 2007)

Partially rescued by

loj⁰⁰⁸⁹⁸ is partially rescued by loj^hs.PC

(Carney and Taylor, 2003)

Not rescued by

loj⁰⁰⁸⁹⁸ is not rescued by loj^UAS.N9d/Scer\GAL4^185Y

(Boltz et al., 2007)

loj⁰⁰⁸⁹⁸ is not rescued by Scer\GAL4^c355/loj^UAS.N9d

(Boltz et al., 2007)

loj⁰⁰⁸⁹⁸ is not rescued by loj^UAS.N9d/Scer\GAL4^c698a

(Boltz et al., 2007)

Comments

Expression of loj^Scer\UAS.N9d in octopaminergic neurons under the control of either Scer\GAL4^ptc-559.1 or Scer\GAL4^C164 rescues the egg deposition defects seen in loj⁰⁰⁸⁹⁸/loj⁰⁴⁰²⁶ mutant females. Expression of loj^Scer\UAS.N9d under the control of the neuropeptidergic driver Scer\GAL4^dimm-929 also rescues the phenotype.

(Saleem et al., 2012)

Expression of loj^Scer\UAS.N9d using Scer\GAL4^CY2 rescues oviposition behaviour and sterility of loj⁰⁰⁸⁹⁸ mutants.

Expression of loj^Scer\UAS.N9d using Scer\GAL4^SG18.1 rescues oviposition behaviour and sterility of loj⁰⁰⁸⁹⁸ and loj⁰⁰⁸⁹⁸/loj⁰⁴⁰²⁶ mutants.

Expression of loj^Scer\UAS.N9d using Scer\GAL4^185Y, Scer\GAL4^c355 or Scer\GAL4^c698a does not rescue oviposition behaviour or sterility of loj⁰⁰⁸⁹⁸ mutants.

(Boltz et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Complete or partial loss of the P{PZ} element is sufficient to rescue the loj mutant phenotype.

(Carney and Taylor, 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

fs(3)00898

(Carney and Taylor, 2003)

loj⁰⁰⁸⁹⁸

(Saleem et al., 2012, Boltz et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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