FB2024_03 , released June 25, 2024
Allele: Dmel\foxoUAS.cKa
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General Information
Symbol
Dmel\foxoUAS.cKa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Kramer
FlyBase ID
FBal0151926
Feature type
allele
Associated gene
Dmel\foxo
Associated Insertion(s)
Carried in Construct
P{UAS-foxo.K}
Key Links
Transgenic product class
cDNA
(Kramer et al., 2003)
wild_type
(Kramer et al., 2003)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Kramer et al., 2003)
wild_type
(Kramer et al., 2003)
Mutagen
in vitro construct
(Kramer et al., 2003)
Progenitor genotype
Carried in construct
P{UAS-foxo.K}
Cytology
Description

UASt regulatory sequences drive expression of a foxo cDNA.

(Kramer et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
foxo
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of foxoScer\UAS.cKa under the control of Scer\GAL4Act5C.PI early in larval development results in complete developmental arrest of the larvae, which remain in the first instar for up to 7 days. The feeding behaviour of these larvae is different from controls; the larvae expressing foxoScer\UAS.cKa under the control of Scer\GAL4Act5C.PI show a 3-4 fold increase in wondering at 48 and 72 hours after egg laying compared to control larvae. Expression of foxoScer\UAS.cKa under the control of Scer\GAL4hs.PB early in larval development using heat shock treatment for 10 minutes every 24 hours inhibits larval growth. This developmental arrest is reversible. Expression of foxoScer\UAS.cKa under the control of Scer\GAL4hs.PB in the third larval instar using heat shock at 37oC for 4 hours results in significant lethality. The rare escapers that do survive to adulthood are smaller than control flies. Expression of foxoScer\UAS.cKa under the control of Scer\GAL4hs.PB at 29oC results in flies which are approximately half the weight of control flies. The wing area is reduced nearly one third, due to a reduction in both cell size and cell number. Eyes show reductions in both ommatidia number and ommatidia area (reflecting cell number and cell size respectively). Expression of foxoScer\UAS.cKa under the control of Scer\GAL4GMR.PF results in eyes that are smaller than normal, and they lack many ommatidia and nearly all of the mechanosensory bristles. The remaining ommatidia are arranged in the typical hexagonal array and all of the normal photoreceptor cells are present. Expression of foxoScer\UAS.cKa under the control of Scer\GAL4GMR.PF results in a significant reduction in the area of the ommatidia compared to control flies.

      (Kramer et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4GMR.PF, foxoUAS.cKa has visible phenotype, non-suppressible by BacA\p35UAS.cHa, Scer\GAL4GMR.PF

      (Kramer et al., 2003)
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      NOT suppressed by
      NOT Suppressor of
      Statement
      Reference

      foxoUAS.cKa, Scer\GAL4GMR.PF is a non-suppressor of ommatidium phenotype of EgfrUAS.cBa, Scer\GAL4GMR.PF

      (Kramer et al., 2003)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of foxoScer\UAS.cKa and Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF almost completely obliterates the eyes. Co-expression of Akt1Scer\UAS.cSa or Pi3K92EScer\UAS.T:Hsap\MYC almost completely rescues the eye phenotype caused by expression of foxoScer\UAS.cKa under the control of Scer\GAL4GMR.PF; the ommatidia and nearly all of the mechanosensory bristles are restored. Co-expression of EgfrScer\UAS.cBa and foxoScer\UAS.cKa under the control of Scer\GAL4GMR.PF does not rescue the eye phenotype seen in animals expressing foxoScer\UAS.cKa alone under the control of Scer\GAL4GMR.PF (ommatidia and bristles are still missing) and the general disorganisation of the ommatidia seen in animals expressing EgfrScer\UAS.cBa alone under the control of Scer\GAL4GMR.PF is also unaffected in the double mutant flies. Co-expression of Ras64BVal14.Scer\UAS is sufficient to restore many of the ommatidia and mechanosensory bristles that are lost in the eyes of animals expressing foxoScer\UAS.cKa.

      (Kramer et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      foxoScer\UAS.cKa
      foxoUAS.cKa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Kramer
      (Kramer et al., 2003)
      Secondary FlyBase IDs
        References (1)