FlyBase Allele Report: Hsap\GPR37[UAS.cYa]

General Information

Symbol

Hsap\GPR37^UAS.cYa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Yang

FlyBase ID

FBal0152460

Feature type

allele

Associated gene

Hsap\GPR37

Associated Insertion(s)

Carried in Construct

P{UAS-Pael-R.Y}

Key Links

Transgenic product class

cDNA

(Yang et al., 2003)

wild_type

(Yang et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Yang et al., 2003)

wild_type

(Yang et al., 2003)

Mutagen

in vitro construct

(Yang et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Pael-R.Y}

Cytology

Description

UASt regulatory sequences drive expression of a full length Hsap\GPR37 cDNA.

(Yang et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\GPR37

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease

CEA

(Umeda-Kameyama et al., 2007, Yang et al., 2003)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Parkinson's disease

is ameliorated by Hsap\PRKN^UAS.cYa

(Yang et al., 2003)

is exacerbated by park^RNAi.UAS

(Yang et al., 2003)

is exacerbated by dhd^P8

(Umeda-Kameyama et al., 2007)

is exacerbated by dhd^J5

(Umeda-Kameyama et al., 2007)

is ameliorated by dhd^UAS.cUa

(Umeda-Kameyama et al., 2007)

is ameliorated by Trx2^UAS.cUa

(Umeda-Kameyama et al., 2007)

is ameliorated by TrxT^UAS.cUa

(Umeda-Kameyama et al., 2007)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior, with Scer\GAL4^elav.PLu

(Umeda-Kameyama et al., 2007)

abnormal neuroanatomy, with Scer\GAL4^Ddc.PL

(Yang et al., 2003)

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(Yang et al., 2003)

visible, with Scer\GAL4^ap-md544

(Yang et al., 2003)

Phenotype Manifest In

adult thorax, with Scer\GAL4^ap-md544

(Yang et al., 2003)

dopaminergic neuron, with Scer\GAL4^Ddc.PL

(Yang et al., 2003)

dopaminergic neuron, with Scer\GAL4^elav.PLu

(Umeda-Kameyama et al., 2007)

dopaminergic neuron, with Scer\GAL4^elav-C155

(Yang et al., 2003)

Detailed Description

Statement

Reference

50 day old flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^elav.PLu show a reduction in average climbing activity compared to 5 day old flies of the same genotype. 50 day old flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^elav.PLu show an approximately 80% reduction in the number of dopaminergic neurons in the dorsomedial clusters of the brain compared to 5 day old flies of the same genotype.

(Umeda-Kameyama et al., 2007)

In 40 day old flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^Ddc.PL, the number of dopaminergic (DA) neurons in the dorsomedial (DM) cluster of the brain is reduced compared to controls. The degree of reduction in the number of DA neurons correlates with the expression level of Hsap\GPR37^Scer\UAS.cYa. The number of DA neurons in the DM clusters of newly eclosed flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^Ddc.PL is similar to that of control flies. The number of DA neurons in the DM clusters is reduced in 40 day old flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^elav-C155. The overall morphology of the central brain is normal in these flies. Retinal architecture and photoreceptor neuron number is also normal. Flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^ey.PH or Scer\GAL4^GMR.PF have normal eye morphology and eye structure. Expression of Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^how-24B or Scer\GAL4^dpp.blk1 does not result in a mutant phenotype. Newly eclosed flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^ap-md544 have body fluid leaking from two spots located in a bilaterally symmetric fashion on the dorsal thorax at the junction between the notum and scutellum. The fluids quickly solidify and form two dark patches adhering to the cuticle.

(Yang et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\GPR37^UAS.cYa has abnormal locomotor behavior phenotype, enhanceable by dhd[+]/dhd^P8/Scer\GAL4^elav.PLu

(Umeda-Kameyama et al., 2007)

Hsap\GPR37^UAS.cYa has abnormal locomotor behavior phenotype, enhanceable by dhd^J5/dhd[+]/Scer\GAL4^elav.PLu

(Umeda-Kameyama et al., 2007)

Hsap\GPR37^UAS.cYa, Scer\GAL4^Ddc.PL has abnormal neuroanatomy phenotype, enhanceable by park^RNAi.UAS, Scer\GAL4^Ddc.PL

(Yang et al., 2003)

Suppressed by

Statement

Reference

Hsap\GPR37^UAS.cYa has abnormal locomotor behavior phenotype, suppressible by Scer\GAL4^elav.PLu/TrxT^UAS.cUa

(Umeda-Kameyama et al., 2007)

Hsap\GPR37^UAS.cYa has abnormal locomotor behavior phenotype, suppressible by Trx2^UAS.cUa/Scer\GAL4^elav.PLu

(Umeda-Kameyama et al., 2007)

Hsap\GPR37^UAS.cYa has abnormal locomotor behavior phenotype, suppressible by dhd^UAS.cUa/Scer\GAL4^elav.PLu

(Umeda-Kameyama et al., 2007)

Hsap\GPR37^UAS.cYa has abnormal locomotor behavior phenotype, suppressible by Scer\GAL4^elav.PLu/Hsap\PRKN^UAS.cYa

(Umeda-Kameyama et al., 2007)

Hsap\GPR37^UAS.cYa has abnormal locomotor behavior phenotype, suppressible by TrxT^{D26A.K57I.UAS}/Scer\GAL4^elav.PLu

(Umeda-Kameyama et al., 2007)

Hsap\GPR37^UAS.cYa has abnormal locomotor behavior phenotype, suppressible by TrxT^C35A.UAS/Scer\GAL4^elav.PLu

(Umeda-Kameyama et al., 2007)

Hsap\GPR37^UAS.cYa, Scer\GAL4^Ddc.PL has abnormal neuroanatomy phenotype, suppressible by Hsap\PRKN^UAS.cYa, Scer\GAL4^Ddc.PL

(Yang et al., 2003)

Hsap\GPR37^UAS.cYa, Scer\GAL4^ap-md544 has visible phenotype, suppressible by Hsap\PRKN^UAS.cYa, Scer\GAL4^ap-md544

(Yang et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\GPR37^UAS.cYa, Scer\GAL4^Ddc.PL has dopaminergic neuron phenotype, enhanceable by park^RNAi.UAS, Scer\GAL4^Ddc.PL

(Yang et al., 2003)

Suppressed by

Statement

Reference

Hsap\GPR37^UAS.cYa has dopaminergic neuron phenotype, suppressible by Scer\GAL4^elav.PLu/TrxT^UAS.cUa

(Umeda-Kameyama et al., 2007)

Hsap\GPR37^UAS.cYa has dopaminergic neuron phenotype, suppressible by Trx2^UAS.cUa/Scer\GAL4^elav.PLu

(Umeda-Kameyama et al., 2007)

Hsap\GPR37^UAS.cYa has dopaminergic neuron phenotype, suppressible by dhd^UAS.cUa/Scer\GAL4^elav.PLu

(Umeda-Kameyama et al., 2007)

Hsap\GPR37^UAS.cYa has dopaminergic neuron phenotype, suppressible by Scer\GAL4^elav.PLu/Hsap\PRKN^UAS.cYa

(Umeda-Kameyama et al., 2007)

Hsap\GPR37^UAS.cYa has dopaminergic neuron phenotype, suppressible by TrxT^{D26A.K57I.UAS}/Scer\GAL4^elav.PLu

(Umeda-Kameyama et al., 2007)

Hsap\GPR37^UAS.cYa has dopaminergic neuron phenotype, suppressible by TrxT^C35A.UAS/Scer\GAL4^elav.PLu

(Umeda-Kameyama et al., 2007)

Hsap\GPR37^UAS.cYa, Scer\GAL4^ap-md544 has adult thorax phenotype, suppressible by Hsap\PRKN^UAS.cYa, Scer\GAL4^ap-md544

(Yang et al., 2003)

Hsap\GPR37^UAS.cYa, Scer\GAL4^Ddc.PL has dopaminergic neuron phenotype, suppressible by Hsap\PRKN^UAS.cYa, Scer\GAL4^Ddc.PL

(Yang et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of one of TrxT^Scer\UAS.cUa, Trx-2^Scer\UAS.cUa, dhd^Scer\UAS.cUa or Hsap\PARK2^Scer\UAS.cYa dramatically improves the average climbing activity of 50 day old flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^elav.PLu.

Co-expression of TrxT^{D26A.K57I.Scer\UAS} or TrxT^{C35A.Scer\UAS} improves the average climbing activity of 50 day old flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^elav.PLu.

Co-expression of one of TrxT^Scer\UAS.cUa, TrxT^{D26A.K57I.Scer\UAS}, TrxT^{C35A.Scer\UAS}, Trx-2^Scer\UAS.cUa, dhd^Scer\UAS.cUa or Hsap\PARK2^Scer\UAS.cYa significantly rescues the reduction in the number of dopaminergic neurons in the dorsomedial clusters of the brain seen in 50 day old flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^elav.PLu.

The reduction in climbing ability seen in 50 day old flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^elav.PLu is made more severe by the addition of dhd^P8/+ or dhd^J5/+.

(Umeda-Kameyama et al., 2007)

Coexpression of Hsap\PARK2^Scer\UAS.cYa suppresses the thoracic degeneration phenotype (leaking body fluid on the dorsal thorax) seen in newly eclosed flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^ap-md544. Coexpression of Hsap\PARK2^Scer\UAS.cYa suppresses the reduction in the number of DA neurons in the DM clusters that is seen in 40 day old flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^Ddc.PL. Coexpression of park^{dsRNA.Scer\UAS} results in a dosage-dependent acceleration of the DA neuron degeneration that is seen in flies expressing Hsap\GPR37^Scer\UAS.cYa under the control of Scer\GAL4^Ddc.PL; flies co-expressing Hsap\GPR37^Scer\UAS.cYa and two copies of park^{dsRNA.Scer\UAS} show the degeneration phenotype at 14 days of age, whereas flies expressing Hsap\GPR37^Scer\UAS.cYa alone or Hsap\GPR37^Scer\UAS.cYa and one copy of park^{dsRNA.Scer\UAS} have a relatively normal number of DA neurons in the DM clusters. The severity of the DA neuron degeneration phenotype is also enhanced.

(Yang et al., 2003)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\GPR37^Scer\UAS.cYa

Hsap\GPR37^UAS.cYa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Yang

(Yang et al., 2003)

Secondary FlyBase IDs

References (2)

Report Sections

Open Close