FlyBase Allele Report: Dmel\ed[Ext.UAS]

General Information

Symbol

Dmel\ed^Ext.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0155343

Feature type

allele

Associated gene

Dmel\ed

Associated Insertion(s)

Carried in Construct

P{UAS-ed.Ext}

Key Links

Transgenic product class

dominant_negative_variant

(Ahmed et al., 2003)

inframe_deletion

(Ahmed et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Ahmed et al., 2003)

inframe_deletion

(Ahmed et al., 2003)

Mutagen

in vitro construct

(Ahmed et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-ed.Ext}

Cytology

Description

UASt regulatory sequences drive expression of amino acids 1-1028 of ed (the entire extracellular region, the entire predicted transmembrane domain and an additional 11 amino acids after the transmembrane domain). Encodes a dominant negative protein.

(Ahmed et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^69B

(Ahmed et al., 2003)

visible, with Scer\GAL4^Eq

(Ahmed et al., 2003)

visible, with Scer\GAL4^pnr-MD237

(Ahmed et al., 2003)

visible, with Scer\GAL4^T80

(Ahmed et al., 2003)

Phenotype Manifest In

microchaeta | ectopic, with Scer\GAL4^Eq

(Ahmed et al., 2003)

microchaeta | ectopic, with Scer\GAL4^pnr-MD237

(Ahmed et al., 2003)

scutellar bristle | ectopic, with Scer\GAL4^pnr-MD237

(Ahmed et al., 2003)

scutellar bristle | ectopic, with Scer\GAL4^T80

(Ahmed et al., 2003)

scutellum, with Scer\GAL4^pnr-MD237

(Ahmed et al., 2003)

wing | distal, with Scer\GAL4^T80

(Ahmed et al., 2003)

wing vein L2, with Scer\GAL4^69B

(Ahmed et al., 2003)

wing vein L2, with Scer\GAL4^T80

(Ahmed et al., 2003)

Detailed Description

Statement

Reference

Animals expressing ed^Ext.Scer\UAS under the control of Scer\GAL4^69B have extra vein material at the distal end of wing vein 2. Animals expressing ed^Ext.Scer\UAS under the control of Scer\GAL4^T80 have extra vein material at the distal end of wing vein 2, and occasionally, notching of the distal wing tip. The scutellum has extra sensory bristles. Animals expressing ed^Ext.Scer\UAS under the control of Scer\GAL4^pnr-MD237 have an increased number of macrochaetae, particularly in the scutellar region. The extra bristle phenotype is accompanied by loss of epidermal tissue, resulting in a smaller scutellum. Microchaetae are duplicated or quadrupled and missing their sockets, or are missing completely. Expression of ed^Ext.Scer\UAS under the control of Scer\GAL4^Eq1 results in a slight increase in the number of microchaetae on the adult thorax.

(Ahmed et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Eq, ed^Ext.UAS has visible phenotype, enhanceable by Delta^UAS.cDa, Scer\GAL4^Eq

(Ahmed et al., 2003)

Enhancer of

Statement

Reference

ed^Ext.UAS, Scer\GAL4^Eq is an enhancer of visible phenotype of Delta^UAS.cDa, Scer\GAL4^Eq

(Ahmed et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Eq, ed^Ext.UAS has microchaeta | ectopic phenotype, enhanceable by Delta^UAS.cDa, Scer\GAL4^Eq

(Ahmed et al., 2003)

Enhancer of

Statement

Reference

ed^Ext.UAS, Scer\GAL4^Eq is an enhancer of microchaeta | ectopic phenotype of Delta^UAS.cDa, Scer\GAL4^Eq

(Ahmed et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of both Dl^Scer\UAS.cDa and ed^Ext.Scer\UAS under the control of Scer\GAL4^Eq1 significantly increases the density of microchaetae on the adult thorax (the increase is greater than an additive combination of the phenotypes seen when Dl^Scer\UAS.cDa or ed^Ext.Scer\UAS are expressed singly using Scer\GAL4^Eq1). Adults expressing both ed^Ext.Scer\UAS and HLHm7^{Scer\UAS.cdCa} simultaneously under the control of Scer\GAL4^pnr-MD237 show the same loss of bristles phenotype as flies expressing HLHm7^{Scer\UAS.cdCa} alone under the control of Scer\GAL4^pnr-MD237.

(Ahmed et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ed^Ext.Scer\UAS

ed^Ext.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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