Expression of Sod1^{dsRNA.Scer\UAS.cHa} RNAi under the control of Scer\GAL4^da.PU strongly reduces the survival rate of adult flies compared to controls.

Expression via P{GAL4-tim.E}27 or P{cry-GAL4.Z}17 (in the presence of Dcr-2^Scer\UAS.cDa) does not significantly affect circadian rhythmicity in locomotor activity assays in constant darkness.

Flies expressing Sod^{dsRNA.Scer\UAS.cHa} under the control of Scer\GAL4^{Hand.ΔVM.Switch} show no significant difference in heart function compared to controls.

Flies expressing Sod^{dsRNA.Scer\UAS.cHa} under the control of Scer\GAL4^da.G32 are short lived and highly sensitive to paraquat. However, lifespan changes in response to diet in males, but not in females, expressing Sod^{dsRNA.Scer\UAS.cHa} differ from that of control flies. Diet containing relative high sugar and low protein levels (HS-LP) results in a slight increase (16.5%) in mean lifespan of Sod^{dsRNA.Scer\UAS.cHa}-expressing males relative to the base diet, and decreased lifespan by 49.6% relative to a low sugar, low protein diet (L-C). In contrast, compared with the base diet, wild-type flies show an increase in mean lifespan in response to both HS-LP and L-C.

Antioxidant treatment in the form of N-acetylcysteine (NAC) improves the survival of males expressing Sod^{dsRNA.Scer\UAS.cHa} under the control of Scer\GAL4^da.G32.

Compared with wild-type, expression of Sod^{dsRNA.Scer\UAS.cHa} using a gut-specific driver (Scer\GAL4^cad-md509) is not sufficient to alter the lifespan of flies responding to diet.

Lifespan patterns in control males and females without RU486 induction of Sod^{dsRNA.Scer\UAS.cHa} expression using Scer\GAL4^da.Switch.PT in response to diet are similar to that of wild-type (Canton S). The HS-LP diet shortened lifespan in males with adult-onset Sod^{dsRNA.Scer\UAS.cHa}-expression relative to the L-C diet and slightly to the base diet. However, the HS-LP diet shortened lifespan in females with adult-onset Sod^{dsRNA.Scer\UAS.cHa}-expression relative to the L-C diet only.

In wild-type males, rapamycin treatment slightly increases the lifespan of animals fed the base diet and decreases the lifespan of animals fed the L-C diet, but it does not alter lifespan in males fed the HS-LP diet, relative to diet-matched non-supplemented controls. Rapamycin increases lifespan in control females fed the base and L-C dites but not the HS-LP diet. In contrast, rapamycin increases lifespan both in males and females expressing Scer\GAL4^da.G32>Sod^{dsRNA.Scer\UAS.cHa} on all three diets relative to genotype-matched non-supplemented controls. Rapamycin-fed Scer\GAL4^da.G32>Sod^{dsRNA.Scer\UAS.cHa} males on the L-C diet are still longest lived relative to those on the base and HS-LP diets.

The expression of Sod1^{dsRNA.Scer\UAS.cHa} under the control of Scer\GAL4^da.G32 results in a severe decrease in life span, as compared to controls. Similar to the findings in wild-type flies, food-supplementation with up to 4% nectarine does not consistently extend the mean and maximum life span of males expressing Sod1^{dsRNA.Scer\UAS.cHa} under the control of Scer\GAL4^da.G32. However, supplementation with nectarine at 2 or 4% significantly increases the mean and maximumlife span of females expressing Sod1^{dsRNA.Scer\UAS.cHa} under the control of Scer\GAL4^da.G32.

Expression of Sod^{dsRNA.Scer\UAS.cHa} under the control of Scer\GAL4^da.G32 severely sensitises flies to iron toxicity.

The expression of Sod1^{dsRNA.Scer\UAS.cHa} under the control of Scer\GAL4^da.G32 leads a significant reduction in adult lifespan, as compared to controls.

Chronic hypoxia (5% O[[2]]) dramatically restores the abbreviated life span of flies expressing Sod^{dsRNA.Scer\UAS.cHa} under the control of Scer\GAL4^da.G32.

Animals expressing Sod^{dsRNA.Scer\UAS.cHa} under the control of Scer\GAL4^da.G32 which are switched from normoxia into hypoxia at a point at which approximately 50% of the population have died continue to die as if they have remained in normoxia throughout.

Scer\GAL4^sca.PU/Sod1^RNAi.UAS.cHa is a suppressor of visible phenotype of Scer\GAL4^sca.PU, Vap33^UAS.cRa

Sod1^RNAi.UAS.cHa, Scer\GAL4^elav-C155 is a suppressor of short lived phenotype of Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155