FB2024_03 , released June 25, 2024
Allele: Dmel\rtRNAi.UAS
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General Information
Symbol
Dmel\rtRNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0158710
Feature type
allele
Associated gene
Dmel\rt
Associated Insertion(s)
Carried in Construct
P{UAS-rt.RNAi}
Key Links
Transgenic product class
RNAi_reagent
(Ichimiya et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Ichimiya et al., 2004)
Mutagen
in vitro construct
(Ichimiya et al., 2004)
Progenitor genotype
Carried in construct
P{UAS-rt.RNAi}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat of nucleotides 67-566 of the coding sequence of rt.

(Ichimiya et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
rt
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  Walker-Warburg syndrome
      CEA
      (Ueyama et al., 2010)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Adults expressing rtdsRNA.Scer\UAS under the control of Scer\GAL4Act5C.PU have significantly decreased climbing ability compared to control flies.

      Expression of rtdsRNA.Scer\UAS under the control of Scer\GAL4Act5C.PU results in some lethality, which increases as the temperature is increased.

      Expression of rtdsRNA.Scer\UAS under the control of Scer\GAL4Act5C.PU, but not Scer\GAL4elav.PU or Scer\GAL4repo, results in a significant decrease in the median lifespan of flies compared to controls.

      (Ueyama et al., 2010)

      At 25oC, flies expressing rtdsRNA.Scer\UAS under the control of Scer\GAL4Act5C.PHb have a viability of 19%, while at 28oC, the viability is 0%. Adult escapers expressing rtdsRNA.Scer\UAS under the control of Scer\GAL4Act5C.PHb show a clockwise twisted abdomen phenotype (the phenotype is seen in both males and females).

      (Ichimiya et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      rtRNAi.UAS, Scer\GAL4en-e16E is an enhancer of visible phenotype of DgRNAi.UAS.cUa, Scer\GAL4en-e16E

      (Ueyama et al., 2010)
      Other
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      rtRNAi.UAS, Scer\GAL4en-e16E is an enhancer of wing phenotype of DgRNAi.UAS.cUa, Scer\GAL4en-e16E

      (Ueyama et al., 2010)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The penetrance of the blistered wing phenotype caused by expression of DgdsRNA.Scer\UAS.cUa under the control of Scer\GAL4en-e16E is significantly enhanced by co-expression of rtdsRNA.Scer\UAS at 28[o]C.

      (Ueyama et al., 2010)

      Animals expressing both Pomt2dsRNA.Scer\UAS and rtdsRNA.Scer\UAS under the control of Scer\GAL4Act5C.PHb show complete lethality.

      (Ichimiya et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      rtRNAi.UAS
      rtdsRNA.Scer\UAS
      rtdsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (3)