FB2024_03 , released June 25, 2024
Allele: Dmel\mio1
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General Information
Symbol
Dmel\mio1
Species
D. melanogaster
Name
FlyBase ID
FBal0158955
Feature type
allele
Associated gene
Dmel\mio
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

antimorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

antimorphic allele - genetic evidence

(Iida and Lilly, 2004)
Mutagen
ethyl methanesulfonate
(Iida and Lilly, 2004)
Progenitor genotype
Cytology
Description

Amino acid replacement: W110term.

(Iida and Lilly, 2004)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:2,217,866..2,217,866 [+]
Nucleotide change:

G2217866A

Amino acid change:

W110term | mio-PA

Reported amino acid change:

W110term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Iida and Lilly, 2004)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Egg chambers from mio1/mio2 females often develop without an oocyte and cease to develop beyond stage 5 or 6 of oogenesis.

      (Wei et al., 2014)

      In mio1/mio2 mutants, the vast majority of egg chambers arrest prior to stage 5 of oogenesis, well before the start of vitellogenesis in stage 7.

      (Senger et al., 2011)

      81% of egg chambers in mio1 homozygotes have 16-polyploid nurse cells and no oocyte. These egg chambers rarely develop beyond stage 5 of oogenesis. This is a higher penetrance than seen for mio1/Df(2L)yan-J2 (64%), suggesting that mio1 is a weak antimorph.

      (Iida and Lilly, 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      mio2/mio1 has egg chamber phenotype, suppressible by Scer\GAL4nanos.PG/Nprl2RNAi.UAS.V20

      (Wei et al., 2014)

      mio2/mio1 has egg chamber phenotype, suppressible by Scer\GAL4nanos.PG/Nprl3RNAi.UAS.V20

      (Wei et al., 2014)

      mio2/mio1 has egg chamber phenotype, suppressible by Scer\GAL4nanos.PG/Tsc1GL00012

      (Wei et al., 2014)

      mio2/mio1 has egg chamber phenotype, suppressible | partially by Nup44A[+]/Nup44AEP2417

      (Senger et al., 2011)

      mio2/mio1 has egg chamber phenotype, suppressible by Df(2R)ED1735/+

      (Senger et al., 2011)

      mio2/mio1 has egg chamber phenotype, suppressible by Nup44AΔ15/Nup44A[+]

      (Senger et al., 2011)

      mio2/mio1 has nurse cell phenotype, suppressible | partially by mei-W68[+]/mei-W681

      (Iida and Lilly, 2004)

      mio2/mio1 has nurse cell phenotype, suppressible | partially by mei-W68[+]/mei-W68k05603

      (Iida and Lilly, 2004)

      mio2/mio1 has oocyte phenotype, suppressible | partially by mei-W68[+]/mei-W68k05603

      (Iida and Lilly, 2004)

      mio2/mio1 has oocyte phenotype, suppressible | partially by mei-W68[+]/mei-W681

      (Iida and Lilly, 2004)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Nup44AEP2417/+ partially suppresses the mio1/mio2 oogenesis phenotype. In contrast to mio1/mio2 single mutants, egg chambers from Nup44AEP2417/+, mio1/mio2 females frequently progress through vitellogenesis to produce mature eggs. Moreover, a small percentage of the eggs laid by Nup44AEP2417/+, mio1/mio2 females hatch and develop into viable adults.

      The percentage of mio1/mio2 mutant egg chambers with an oocyte increases 6-fold in a Df(2R)ED1735/+ genetic background.

      The percentage of mio1/mio2 mutant egg chambers with an oocyte increases 6-fold in a Nup44AΔ15/+ genetic background.

      (Senger et al., 2011)

      Transformation of oocytes to nurse cells in mio2/mio1 females is suppressed by mei-W681/+ or mei-W68k05603/+.

      (Iida and Lilly, 2004)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Identified in a screen for mutants that alter the cell cycle of the ovarian cyst.

      (Iida and Lilly, 2004)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Mio1
      (Gu et al., 2022)
      mio1
      (Cai et al., 2016, Wei et al., 2014, Senger et al., 2011)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)