FlyBase Allele Report: Dmel\Nos[4.GMR.Tag:FLAG]

General Information

Symbol

Dmel\Nos^{4.GMR.Tag:FLAG}

Species

D. melanogaster

Name

FlyBase ID

FBal0160130

Feature type

allele

Associated gene

Dmel\Nos

Associated Insertion(s)

Carried in Construct

P{GMR-Nos.4.FLAG}

Key Links

Transgenic product class

cDNA

(Stasiv et al., 2004)

wild_type

(Stasiv et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Stasiv et al., 2004)

wild_type

(Stasiv et al., 2004)

Mutagen

in vitro construct

(Stasiv et al., 2004)

Progenitor genotype

Carried in construct

P{GMR-Nos.4.FLAG}

Cytology

Description

A Nos dNOS4 isoform cDNA is tagged with two copies of Tag:FLAG at the 3' end and expressed under the control of GMR regulatory sequences.

(Stasiv et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

Nos

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | adult stage

(Stasiv et al., 2004)

increased occurrence of cell division | larval stage

(Stasiv et al., 2004)

Phenotype Manifest In

cell cycle

(Stasiv et al., 2004)

eye

(Stasiv et al., 2004)

interommatidial bristle

(Stasiv et al., 2004)

secondary pigment cell

(Stasiv et al., 2004)

tertiary pigment cell

(Stasiv et al., 2004)

Detailed Description

Statement

Reference

Mutant animals exhibit a distorted eye phenotype with visible rearrangements of the ommatidia lattice and extra bristles. Mutant animals have extra secondary and tertiary pigment cells as well as extra bristle cells. No change is seen in the number of photoreceptor cells. The extra cells distort the regular hexagonal shape of the ommatidia and the general lattice pattern of the retina. When mutant imaginal disc are imagined a 2.13 fold increase in the number of cells in S phase are seen. These cells are seen in the morphogenetic furrow and the area posterior to it.

(Stasiv et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Nos^{4.GMR.Tag:FLAG} is an enhancer of ommatidium phenotype of BacA\p35^GMR.PH, Dp^GMR.PD, E2f1^GMR.PD

(Stasiv et al., 2004)

Nos^{4.GMR.Tag:FLAG} is an enhancer of eye phenotype of BacA\p35^GMR.PH, Dp^GMR.PD, E2f1^GMR.PD

(Stasiv et al., 2004)

Suppressor of

Statement

Reference

Nos^{4.GMR.Tag:FLAG} is a suppressor of interommatidial bristle phenotype of Rbf^GMR.PD

(Stasiv et al., 2004)

Nos^{4.GMR.Tag:FLAG} is a suppressor of ommatidium phenotype of Rbf^GMR.PD

(Stasiv et al., 2004)

Nos^{4.GMR.Tag:FLAG} is a suppressor of pigment cell phenotype of Rbf^GMR.PD

(Stasiv et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Nos^{4.GMR.T:Zzzz\FLAG}

Nos^{4.GMR.Tag:FLAG}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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