FlyBase Allele Report: Dmel\Nf1[c00617]

General Information

Symbol

Dmel\Nf1^c00617

Species

D. melanogaster

Name

FlyBase ID

FBal0160134

Feature type

allele

Associated gene

Dmel\Nf1

Associated Insertion(s)

PBac{PB}Nf1^c00617

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

piggyBac activity

Nature of the Allele

Allele class

Mutagen

piggyBac activity

(Bloomington Drosophila Stock Center, 2004.11.6)

Progenitor genotype

Associated Insertion(s)

PBac{PB}Nf1^c00617

Cytology

Description

Allele components

Component

Use(s)

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:25,986,098..25,986,098 [+]

(Buchanan and Davis, 2010, Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory

(Buchanan and Davis, 2010)

abnormal sleep (with Nf1^P2)

(Bai and Sehgal, 2015)

decreased body size | male limited

(Buchanan and Davis, 2010)

fertile

(Bloomington Drosophila Stock Center, 2004.11.6)

viable

(Bloomington Drosophila Stock Center, 2004.11.6)

Phenotype Manifest In

Detailed Description

Statement

Reference

Nf1^P2/Nf1^c00617 male or female flies sleep significantly less than wild type.

(Bai and Sehgal, 2015)

Nf1^c00617 males are smaller than control males, while females show no change.

Nf1^c00617 flies exhibit 3 minute, 3 hour, and 24 hour memory deficits. In training trial assays, where flies are repeatedly trained with odor avoidance assays, Nf1^P1 flies perform poorly even after one trial, but then exhibit wild-type levels of olfactory memory acquisition. When Nf1^c00617 mutants and controls are both trained with three trials, immediate memory is identical (when performance is normalised to the control so memory decay can be compared). Likewise, memory tested at two subsequent time points is indistinguishable.

(Buchanan and Davis, 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Nf1^c00617 is rescued by Scer\GAL4^elav-C155/Nf1^UAS.cBa

(Buchanan and Davis, 2010)

Nf1^c00617 is rescued by Nf1^UAS.cBa/Scer\GAL4^{Mef2.247.Switch}

(Buchanan and Davis, 2010)

Nf1^c00617 is rescued by Scer\GAL4^Mz1081/Nf1^UAS.cBa

(Buchanan and Davis, 2010)

Nf1^c00617 is rescued by Scer\GAL4^NP0009/Nf1^UAS.cBa

(Buchanan and Davis, 2010)

Nf1^c00617 is rescued by Nf1^UAS.cBa/Scer\GAL4^c739

(Buchanan and Davis, 2010)

Nf1^c00617 is rescued by Scer\GAL4^c747/Nf1^UAS.cBa

(Buchanan and Davis, 2010)

Not rescued by

Nf1^c00617 is not rescued by Nf1^UAS.cBa/Scer\GAL4^c305a

(Buchanan and Davis, 2010)

Nf1^c00617 is not rescued by Nf1^UAS.cBa/Scer\GAL4^NP1131

(Buchanan and Davis, 2010)

Nf1^c00617 is not rescued by Nf1^UAS.cBa/Scer\GAL4^17d

(Buchanan and Davis, 2010)

Nf1^c00617 is not rescued by Scer\GAL4^c747/Scer\GAL80^Mef2.mb247/Nf1^UAS.cBa

(Buchanan and Davis, 2010)

Comments

Expression of Nf1^Scer\UAS.cBa in mushroom body neurons during development and adulthood under the control of inducible Scer\GAL4^{Mef2.247.Switch} (in a RU486 environment) completely rescues the 3 hour memory phenotype seen in Nf1^c00617 mutants. Expression only during adulthood is sufficient for complete rescue.

Expression of Nf1^Scer\UAS.cBa in α'/β' neurons under the control of Scer\GAL4^c305a is not sufficient to rescue the 3 hour memory phenotype of Nf1^c00617 homozygous mutants.

Expression of Nf1^Scer\UAS.cBa in γ neurons under the control of Scer\GAL4^NP1131 is not sufficient to rescue the 3 hour memory phenotype of Nf1^c00617 homozygous mutants.

Expression of Nf1^Scer\UAS.cBa throughout the central brain under the control of Scer\GAL4^Mz1081 is sufficient to rescue the 3 hour memory phenotype found in Nf1^c00617 mutants.

Expression of Nf1^Scer\UAS.cBa throughout the central brain under the control of Scer\GAL4^NP0009 is sufficient to rescue the 3 hour memory phenotype found in Nf1^c00617 mutants.

Expression of Nf1^Scer\UAS.cBa in the α/β mushroom body neurons under the control of Scer\GAL4^c739 is sufficient to rescue the 3 hour memory phenotype found in Nf1^c00617 mutants.

Expression of Nf1^Scer\UAS.cBa in the central core of α/β mushroom body neurons under the control of Scer\GAL4^17d is not sufficient to rescue the 3 hour memory phenotype found in Nf1^c00617 mutants.

The Nf1^c00617 3 hour memory phenotype is fully rescued when Scer\GAL4^c747 is used to express Nf1^Scer\UAS.cBa. However, flies also carrying Scer\GAL80^Mef2.PT exhibit a performance score that is indistinguishable from Nf1^c00617 homozygous mutants.

(Buchanan and Davis, 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

10201

w¹¹¹⁸; PBac{PB}Nf1^c00617

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Nf1^c00617

(Bai and Sehgal, 2015)

nf1^c00617

(Buchanan and Davis, 2010)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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