FlyBase Allele Report: Dmel\Pak[AID.UAS.Tag:FLAG]

General Information

Symbol

Dmel\Pak^{AID.UAS.Tag:FLAG}

Species

D. melanogaster

Name

FlyBase ID

FBal0176054

Feature type

allele

Associated gene

Dmel\Pak

Associated Insertion(s)

Carried in Construct

P{UAS-Pak.AID.FLAG}

Also Known As

UAS-Pak-AID

Key Links

Transgenic product class

inframe_deletion

(Conder et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Conder et al., 2004)

Mutagen

in vitro construct

(Conder et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-Pak.AID.FLAG}

Cytology

Description

UASt sequences drive expression of amino acids His94 to Gly191 of the Pak gene tagged with Tag:FLAG.

(Conder et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Pak

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage, with Scer\GAL4^332.3

(Conder et al., 2004)

lethal - all die during embryonic stage, with Scer\GAL4^c381

(Conder et al., 2004)

lethal - all die during embryonic stage, with Scer\GAL4^LE

(Conder et al., 2004)

some die during embryonic stage, with Scer\GAL4^332.3

(Conder et al., 2004)

some die during embryonic stage, with Scer\GAL4^c381

(Conder et al., 2004)

some die during embryonic stage, with Scer\GAL4^LE

(Conder et al., 2004)

Phenotype Manifest In

Detailed Description

Statement

Reference

When Pak^{AID.Scer\UAS.T:Zzzz\FLAG} is driven by Scer\GAL4^LE a a high frequency of embryonic lethality is seen, with 44% of embryo failing to form cuticle. Of the embryos that form cuticle 21% demonstrate dorsal closure defects in the form of small holes in the dorsal surface of the embryos. Irregularities are seen in cell shape change in the epidermis. Animals expressing Pak^{AID.Scer\UAS.T:Zzzz\FLAG} driven by Scer\GAL4^GMR.PF have abnormally thick axon bundles in the medulla neuropil in the developing eye and axons terminate in poorly differentiated growth cones. When Pak^{AID.Scer\UAS.T:Zzzz\FLAG} is driven by Scer\GAL4^c381 a high frequency of embryonic lethality is seen, with more than half of the embryos failing to secrete cuticle. Of those embryos that do secrete cuticle, about 25% have large holes in the dorsal surface. Mutants also exhibit defects in head involution and incomplete or "puckered" dorsal closure. However cell elongation of the lateral epidermal cells appear normal. When Pak^{AID.Scer\UAS.T:Zzzz\FLAG} is driven by Scer\GAL4^332.3 a high frequency of embryonic lethality is seen, with more than half of the embryos failing to secrete cuticle. Of those embryos that do secrete cuticle, about 25% have large holes in the dorsal surface. The amnioserosa appears somewhat abnormal, but apical constriction of amnioserosa cells occurs as in wild-type.

(Conder et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Pak^{AID.UAS.Tag:FLAG}/Scer\GAL4^332.3 is a suppressor | partially of majority die during embryonic stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Pak^{AID.UAS.Tag:FLAG}, Scer\GAL4^hs.PB is a non-enhancer of amnioserosa phenotype of Rac1^V12.UAS, Scer\GAL4^hs.PB

(Conder et al., 2004)

Suppressor of

Statement

Reference

Pak^{AID.UAS.Tag:FLAG}/Scer\GAL4^332.3 is a suppressor | partially of embryo | dorsal closure stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

Pak^{AID.UAS.Tag:FLAG}/Scer\GAL4^332.3 is a suppressor | partially of embryonic/first instar larval cuticle | embryonic stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

NOT Suppressor of

Statement

Reference

Pak^{AID.UAS.Tag:FLAG}, Scer\GAL4^hs.PB is a non-suppressor of amnioserosa phenotype of Rac1^V12.UAS, Scer\GAL4^hs.PB

(Conder et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Pak^{AID.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^332.3 partially suppresses the embryonic lethality and dorsal closure defects seen in embryos expressing crb^Y10A in a crb^GX24w-/crb^11A22 background, resulting in some adult survival.

(Flores-Benitez and Knust, 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pak^{AID.Scer\UAS.T:Zzzz\FLAG}

Pak^{AID.UAS.Tag:FLAG}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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