FlyBase Allele Report: Dmel\Paip2[UAS.Tag:HA]

General Information

Symbol

Dmel\Paip2^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0176055

Feature type

allele

Associated gene

Dmel\Paip2

Associated Insertion(s)

Carried in Construct

P{UAS-Paip2.HA}

Key Links

Transgenic product class

wild_type

(Roy et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Roy et al., 2004)

Mutagen

in vitro construct

(Roy et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-Paip2.HA}

Cytology

Description

UASt regulatory sequences drive expression of Paip2 tagged with Tag:HA.

(Roy et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Paip2

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell growth, with Scer\GAL4^Bx-MS1096

(Roy et al., 2004)

abnormal size | adult stage, with Scer\GAL4^Bx-MS1096

(Roy et al., 2004)

abnormal size | adult stage, with Scer\GAL4^GMR.PF

(Roy et al., 2004)

abnormal size | larval stage, with Scer\GAL4^Bx-MS1096

(Roy et al., 2004)

decreased cell number | larval stage, with Scer\GAL4^Bx-MS1096

(Roy et al., 2004)

decreased cell size | larval stage, with Scer\GAL4^Act5C.PP

(Roy et al., 2004)

visible, with Scer\GAL4^Bx-MS1096

(Roy et al., 2004)

Phenotype Manifest In

embryonic/larval fat body, with Scer\GAL4^Act5C.PP

(Roy et al., 2004)

ommatidium, with Scer\GAL4^GMR.PF

(Roy et al., 2004)

wing, with Scer\GAL4^Bx-MS1096

(Roy et al., 2004)

wing disc, with Scer\GAL4^Bx-MS1096

(Roy et al., 2004)

Detailed Description

Statement

Reference

When expression is driven in the wing, by Scer\GAL4^Bx-MS1096, wing size is reduced, though pattern is unaffected. The effect on size is evident in the wing disc. The reduction in size is caused by reduction in cell number. The reduction in cell number is not caused by increased apoptosis. Cells take more time than control cells to reach the critical size necessary for cell division. The expression of Paip2 causes an inhibition of cell growth. These effects are related to the number of copies of Paip2^{Scer\UAS.T:Ivir\HA} - the more copies, the greater the effects. When expression is driven in the eye disc, via Scer\GAL4^GMR.PF, the resulting eyes, though normally patterned, have ommatidia reduced in size (not number) compared to those of control eyes. When expression is driven by Scer\GAL4^Act5C.PP, in clones in the larval fat body, the size of the fat body cells is reduced.

(Roy et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Paip2^UAS.Tag:HA, Scer\GAL4^Bx-MS1096 has abnormal cell growth phenotype, suppressible by pAbp^UAS.cSa, Scer\GAL4^Bx-MS1096

(Roy et al., 2004)

Paip2^UAS.Tag:HA, Scer\GAL4^Bx-MS1096 has visible phenotype, suppressible by pAbp^UAS.cSa, Scer\GAL4^Bx-MS1096