FlyBase Allele Report: Dmel\mid[RNAi.UAS]

General Information

Symbol

Dmel\mid^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0178885

Feature type

allele

Associated gene

Dmel\mid

Associated Insertion(s)

Carried in Construct

P{UAS-mid.RNAi}

Also Known As

UAS-nmr2RNAi

Key Links

Transgenic product class

RNAi_reagent

(Qian et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Qian et al., 2005)

Mutagen

in vitro construct

(Qian et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-mid.RNAi}

Cytology

Description

An inverted repeat of mid corresponding to the coding region 5' upstream of the T-box, separated by a w intron, is under the control of UAS sequences. The mid fragment was selected as the region with least homology to H15.

(Qian et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mid

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cardiomyopathy

CEA

(Qian et al., 2008)

model of congenital heart disease

CEA

(Qian et al., 2008)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity, with Scer\GAL4^tin.CΔ4

(Qian et al., 2005)

short lived, with Scer\GAL4^twi.PG

(Qian et al., 2008)

Phenotype Manifest In

adult heart | progressive, with Scer\GAL4^how-24B, Scer\GAL80^ts.αTub84B

(Qian et al., 2008)

adult heart | progressive, with Scer\GAL4^tin.CΔ4, Scer\GAL80^ts.αTub84B

(Qian et al., 2008)

adult heart | progressive, with Scer\GAL4^twi.PG

(Qian et al., 2008)

cardioblast, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Qian et al., 2005)

cardioblast, with Scer\GAL4^tin.CΔ4

(Qian et al., 2005)

embryonic/larval heart, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Qian et al., 2005)

Detailed Description

Statement

Reference

Expression of mid^{dsRNA.Scer\UAS} under the control of Scer\GAL4^twi.PG results in a dramatic increase in the rate of pacing-induced heart arrest/fibrillation, with further increases with age. Down-regulation of mid also results in a severely truncated lifespan.

Heart-specific knockdown of mid through expression of mid^{dsRNA.Scer\UAS} under the control of Scer\GAL80^ts.αTub84B;Scer\GAL4^tin.CΔ4 or Scer\GAL80^ts.αTub84B;Scer\GAL4^how-24B results in an increased rate in pacing-induced heart arrest/fibrillation compared to control flies. The regularity of the myofibrillar alignment and the spacing between the Z-lines is disrupted with the tin-positive myocardium, where mid expression is diminished, but not in the ventral longitudinal muscle fibers that do not expression tin. No notable change in cell death is observed. There is a moderate decrease in diastolic diameter and a mild increase in systolic diameter in mid knockdown flies, resulting in a significant decrease in fractional shortening compared to controls.

(Qian et al., 2008)

Almost one quarter of stage 16/17 embryos expressing mid^{dsRNA.Scer\UAS} throughout the mesoderm, under the control of both Scer\GAL4^how-24B and Scer\GAL4^twi.PG, show defects in heart tube morphogenesis. These embryos exhibit intercalation, clustering and misorientation of myocardial cells. Expression of mid^{dsRNA.Scer\UAS} in differentiating myocardial cells, driven by Scer\GAL4^tin.CΔ4, disrupts the polarity of myocardial cells but does not affect their specification.

(Qian et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^how-24B, Scer\GAL4^twi.PG, mid^RNAi.UAS has embryonic/larval heart phenotype, enhanceable by H15^nmr-614

(Qian et al., 2005)

Scer\GAL4^how-24B, Scer\GAL4^twi.PG, mid^RNAi.UAS has cardioblast phenotype, enhanceable by H15^nmr-614

(Qian et al., 2005)

Other

Statement

Reference

H15^nmr-614, Scer\GAL4^how-24B, Scer\GAL4^twi.PG, mid^RNAi.UAS has cardioblast phenotype

(Qian et al., 2005)

H15^nmr-614, Scer\GAL4^how-24B, Scer\GAL4^twi.PG, mid^RNAi.UAS has embryonic pericardial cell | increased number phenotype

(Qian et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The heart morphogenesis defect of embryos expressing mid^{dsRNA.Scer\UAS} under the control of both Scer\GAL4^how-24B and Scer\GAL4^twi.PG is enhanced in a H15^nmr-614 background, with a greater number of embryos exhibiting misalignment of the myocardial cells. Additionally, these double mutant embryos show a reduction in the number of myocardial cells and a concomitant increase in pericardial cells at stage 14, a phenotype not seen either in H15^nmr-614 or Scer\GAL4^how-24B,Scer\GAL4^twi.PG > mid^{dsRNA.Scer\UAS} single mutants.

(Qian et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into flies to study the phenotypic consequences of dsRNA interference (RNAi) of the mid gene.

(Qian et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mid^RNAi.UAS

mid^{dsRNA.Scer\UAS}

mid^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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