FB2024_03 , released June 25, 2024
Allele: Dmel\cupΔ212
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General Information
Symbol
Dmel\cupΔ212
Species
D. melanogaster
Name
FlyBase ID
FBal0180286
Feature type
allele
Associated gene
Dmel\cup
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
Delta2-3 transposase
(Nakamura et al., 2004)
Progenitor genotype
cup04506
(Nakamura et al., 2004)
Cytology
Description

Imprecise excision of P{PZ}cup04506 has caused a deletion of a 1466 bp. The remaining cup coding region allele is predicted to encode a truncated protein lacking the first 347 amino acids.

(Nakamura et al., 2004)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      cup20/cupΔ212 or cupΔ212/cup8 third instar larvae have significant increases in small extra satellite boutons (for example, budding from parent type 1b boutons) at the (muscle 4) neuromuscular junction (NMJ), compared to wild type or heterozygote controls; Df(2L)BSC7/cupΔ212 show significant increases compared to wild type but not Df(2L)BSC7/+ heterozygotes; cupΔ212/+ heterozygotes show significant increases in boutons compared to wild type.

      cupΔ212/Df(2L)BSC7 third instar larvae do not show significant changes in evoked excitatory postsynaptic potential (EPSP) amplitudes (recordings from muscle 6/7 NMJs), but miniature EPSP (mEPSP) frequency (but not amplitude) is significantly increased compared to controls.

      (Menon et al., 2015)

      cupΔ212 homozygous females lay fragile eggs. The cytoskeletal polarity of cupΔ212 homozygous stage 9 oocytes appears normal.

      (Nakamura et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      Enhancer of
      Statement
      Reference

      cupΔ212/cup[+] is an enhancer of NMJ bouton | third instar larval stage | increased number phenotype of eIF4E1GD1432

      (Menon et al., 2015)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      cupΔ212/+;Df(3L)BSC113/+, cupΔ212/+;EndoAΔ4/+, cupΔ212/+;Dadj1E4/+ or cupΔ212/Df(2L)BSC302 transheterozygotes show significant increases in satellite boutons at the NMJ of third instar larvae compared to either single heterozygote. cupΔ212/Df(2R)X58-7 or cupΔ212/+;Df(3R)3450/+ transheterozygotes do not show significant increases in satellite boutons at the NMJ of third instar larvae compared to either single heterozygote.

      (Menon et al., 2015)

      cupΔ212/+ has a very weak effect on the penetrance of the mutant dorsal appendage phenotype seen in eggs laid by BicC4/+ females, increasing it from 5% to 9% in the double heterozygous flies.

      (Snee and Macdonald, 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      cupΔ212/Df(2L)BSC7 is rescued by Scer\GAL4elav.PU/cupUAS.cMa

      (Menon et al., 2015)
      Comments

      Expression of cupScer\UAS.cMa driven by Scer\GAL4elav.PU significantly rescues the satellite bouton phenotype seen at the NMJ of Df(2L)BSC7/cupΔ212 or Df(2L)BSC7/cup20 third instar larvae; expression driven by Scer\GAL4how-24B fails to rescue the phenotype in Df(2L)BSC7/cup20 larvae.

      (Menon et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      cupΔ212
      (Menon et al., 2015, Snee and Macdonald, 2009, Tadros et al., 2007, Chekulaeva et al., 2006)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)