Expression of NPFR1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav.PU significantly increases ethanol preference in mated males, but not in virgin males.

The memory performance of NPFR1^{dsRNA.Scer\UAS};Scer\GAL4^n-syb.PS flies is impaired. These flies are normal for aversive olfactory conditioning.

Food deprived NPFR1^{dsRNA.Scer\UAS};Scer\GAL4^c061 flies exhibit an impaired appetitive memory.

Expression of NPFR1^{dsRNA.Scer\UAS} under the control of Scer\GAL80^ple.PK or Scer\GAL4^c061 in food-deprived flies impairs 3hr appetitive memory performance. Expression of NPFR1^{dsRNA.Scer\UAS} in all Scer\GAL4^c061 neurons except the DA neurons (through the presence of Scer\GAL80^ple.PK) does not affect appetitive memory.

Mutant animals are significantly more resistant to the sedative effect of ethanol vapour than controls.

Third instar larvae that have fasted for 120 minutes that express NPFR1^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^NPFR1.6.6 or in the nervous system (Scer\GAL4^elav-C155 or Scer\GAL4^Appl.G1a) are deficient in motivated feeding of solid but not liquid food. In contrast, expression of NPFR1^{dsRNA.Scer\UAS} in muscle cells Scer\GAL4^Mhc.PW results in normal feeding responses.