FlyBase Allele Report: Dmel\Lk6[EP30.18]

General Information

Symbol

Dmel\Lk6^EP30.18

Species

D. melanogaster

Name

FlyBase ID

FBal0182925

Feature type

allele

Associated gene

Dmel\Lk6

Associated Insertion(s)

P{EP}EP30.18

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{EP}EP30.18

Cytology

Description

P{EP} insertion approximately 1950bp upstream of the Lk6 open reading frame encoding the shorter isoform.

(Reiling et al., 2005)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size, with Scer\GAL4^da.G32

(Reiling et al., 2005)

decreased cell number, with Scer\GAL4^da.G32

(Reiling et al., 2005)

lethal, with Scer\GAL4^Act5C.PU

(Reiling et al., 2005)

Phenotype Manifest In

wing, with Scer\GAL4^da.G32

(Reiling et al., 2005)

Detailed Description

Statement

Reference

Flies expressing Lk6^EP30.18 under the control of Scer\GAL4^da.G32 are smaller than normal (weight reduction compared to controls is 10% in females and 9% in males). The wings of these flies are smaller than normal, and this is caused by a decrease in cell number, but not in cell size. The weight reduction caused by overexpression of Lk6^EP30.18 under the control of Scer\GAL4^da.G32 is diminished by reducing the yeast content of the food. The lethality caused by overexpression of Lk6^EP30.18 under the control of Scer\GAL4^Act5C is substantially suppressed if the animals are reared on low yeast food. Under starvation conditions, flies expressing Lk6^EP30.18 under the control of Scer\GAL4^da.G32 show an extension of lifespan compared to controls.

(Reiling et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Lk6^EP30.18, Scer\GAL4^da.G32 has decreased body size phenotype, suppressible by eIF4E1^S058911/eIF4E1^{Ser251Ala.t8.9}, Scer\GAL4^da.G32

(Reiling et al., 2005)

Suppressor of

Statement

Reference

Lk6^EP30.18 is a suppressor of increased body size phenotype of Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF

(Reiling et al., 2005)

Phenotype Manifest In

Suppressor of

Statement

Reference

Lk6^EP30.18 is a suppressor of eye phenotype of Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF

(Reiling et al., 2005)

Scer\GAL4^GMR.PF, Lk6^EP30.18 is a suppressor of eye phenotype of Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF

(Reiling et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Animals expressing both Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF have a large-eye phenotype. Expression of Lk6^EP30.18 in this background strongly suppresses this phenotype. The small body phenotype caused by expression of Lk6^EP30.18 under the control of Scer\GAL4^da.G32 is not seen if the flies are also expressing eIF-4E^{Ser251Ala.t8.9} in a eIF-4E^S058911/eIF-4E^S058911 background.

(Reiling et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Lk6^EP30.18

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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