FB2024_03 , released June 25, 2024
Allele: Dmel\Hsp27UAS.Tag:HA
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General Information
Symbol
Dmel\Hsp27UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0183168
Feature type
allele
Associated gene
Dmel\Hsp27
Associated Insertion(s)
Carried in Construct
P{UAS-Hsp27.HA}
Also Known As
UAS-hsp27
Key Links
Transgenic product class
cDNA
(Wang et al., 2004)
wild_type
(Wang et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Wang et al., 2004)
wild_type
(Wang et al., 2004)
Mutagen
in vitro construct
(Wang et al., 2004)
Progenitor genotype
Carried in construct
P{UAS-Hsp27.HA}
Cytology
Description

UAS regulatory sequences drive expression of full length Hsp27 (from EST LD06811) tagged at the C-terminal end with Tag:HA.

(Wang et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hsp27
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  neurodegenerative disease
      modeled by Zzzz\CAG41Q.UAS.Tag:HA
      (Chen et al., 2012)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing Hsp27Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU have a normal eye phenotype.

      (Chen et al., 2012)

      Overexpression of Hsp27Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PI fails to protect against tachypacing-induced atrial fibrillation. Tachypacing also engenders heart dysfunction in wild-type flies.

      (Zhang et al., 2011)

      Expression of Hsp27Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4hs.PB at either 25 or 29oC in male flies results in an increase in mean lifespan compared to controls. Expression of Hsp27Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4da.G32 at 29oC in male flies results in an increase in mean lifespan compared to controls. Expression of Hsp27Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4hs.PB in male flies results in an increased resistance to paraquat and heat stress compared to controls, and a marginal increase in starvation resistance compared to controls. Females expressing Hsp27Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4hs.PB show a reduction in fecundity compared to controls, although there is no significant difference in hatching between eggs derived from these females and those derived from controls.

      (Wang et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      Hsp27UAS.Tag:HA, Scer\GAL4GMR.PU is a suppressor of eye phenotype of Scer\GAL4GMR.PU, Zzzz\CAG41Q.UAS.Tag:HA

      (Chen et al., 2012)
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Flies expressing both Atg7Scer\UAS.cCa and Hsp27Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU have a normal eye phenotype.

      Co-expression of Hsp27Scer\UAS.T:Ivir\HA1 does not rescue the rough eye phenotype caused by expression of Atg7GD11671 under the control of Scer\GAL4GMR.PU.

      (Chen et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference

      The mild mosaic eye pigmentation defect caused by expression of Zzzz\CAG41Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU is rescued by co-expression of Hsp27Scer\UAS.T:Ivir\HA1.

      The rough eye defect caused by expression of Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU is not rescued by co-expression of Hsp27Scer\UAS.T:Ivir\HA1.

      (Chen et al., 2012)
      Complementation and Rescue Data
      Partially rescues

      Hsp27UAS.Tag:HA/Scer\GAL4da.G32 partially rescues Hsp27KG03935

      (Chen et al., 2010)
      Comments

      Expression of Hsp27Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4da.G32 largely rescues the increased susceptibility of Hsp27KG03935 flies to infection with Enterobacter cloacae, Listeria monocytogenes or Beauveria bassiana.

      (Chen et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsp27Scer\UAS.T:Ivir\HA1
      Hsp27UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (4)