FlyBase Allele Report: Dmel\CrebB[cor.UAS]

General Information

Symbol

Dmel\CrebB^cor.UAS

Species

D. melanogaster

Name

corrected

FlyBase ID

FBal0184295

Feature type

allele

Associated gene

Dmel\CrebB

Associated Insertion(s)

Carried in Construct

P{UAS-CrebB-17A-a.cor}

Also Known As

UAS-CrebB-a

Key Links

Transgenic product class

wild_type

(Perazzona et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Perazzona et al., 2004)

Mutagen

in vitro construct

(Perazzona et al., 2004)

Progenitor genotype

CrebB^mut.UAS

(Perazzona et al., 2004)

Carried in construct

P{UAS-CrebB-17A-a.cor}

Cytology

Description

Coding region for CrebB is corrected to wild type sequence by replacement of first 139bp of the mutated CrebB cassette in CrebB^mut.UAS with wild type sequences from the CrebB isoform b. Expression is governed by UAS regulatory sequences.

(Perazzona et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

CrebB

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory, with Scer\GAL4^hs.PB

(Perazzona et al., 2004)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav.PLu

(Chen and Ganetzky, 2012)

lethal | heat sensitive, with Scer\GAL4^hs.PB

(Perazzona et al., 2004)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav.PLu

(Chen and Ganetzky, 2012)

NMJ bouton | third instar larval stage, with Scer\GAL4^elav.PLu

(Chen and Ganetzky, 2012)

Detailed Description

Statement

Reference

Overexpression of CrebB-17A^cor.Scer\UAS driven by Scer\GAL4^elav.PLu causes a mild, but significant, increase in synaptic bouton numbers at larval neuromuscular junctions.

(Chen and Ganetzky, 2012)

Heat shock induced expression of CrebB-17A^cor.Scer\UAS causes a slow lethality. Four days after 30 min heat shock at 37^oC over 50% of the flies were dead, with lethality reaching 99% by day 7. Without heat shock, though in combination Scer\GAL4^hs.PB with which nevertheless increases expression of CrebB-17A^cor.Scer\UAS, the positive effect of 5x spaced training in the olfactory classical conditioning LTM assay (tested at 4 days) is abolished. Thus expression depresses learning in this assay.

(Perazzona et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

CrebB^cor.UAS has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Df(1)Exel9051

(Chen and Ganetzky, 2012)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

CrebB^cor.UAS has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Df(1)Exel9051

(Chen and Ganetzky, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Overexpression of CrebB-17A^cor.Scer\UAS under the control of Scer\GAL4^elav.PLu in a Df(1)Exel9051/Y genetic background results in a mild neuromuscular junction (NMJ) overgrowth phenotype.

(Chen and Ganetzky, 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9232

P{UAS-CrebB-17A-a.cor}T7.1, ry⁵⁰⁶

9233

P{UAS-CrebB-17A-a.cor}T25.4, ry⁵⁰⁶

Notes on Origin

Discoverer

Comments

Full length CrebB-17A product is encoded by the allele.

(Perazzona et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CrebB-17A^cor.Scer\UAS

CrebB^cor.Scer\UAS

CrebB^cor.UAS

Name Synonyms

corrected

(Perazzona et al., 2004)

Secondary FlyBase IDs

References (9)

Report Sections

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