FlyBase Allele Report: Dmel\CTCF[EY15833]

General Information

Symbol

Dmel\CTCF^EY15833

Species

D. melanogaster

Name

FlyBase ID

FBal0184453

Feature type

allele

Associated gene

Dmel\CTCF

Associated Insertion(s)

P{EPgy2}CTCF^EY15833

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Macdonald et al., 2010)

Mutagen

P-element activity

(Mohan et al., 2007, Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EPgy2}CTCF^EY15833

Cytology

Description

The P-element in P{EPgy2}CTCF^EY15833 harbors the y gene reporter adjacent to the 5' region of CTCF and the w gene reporter adjacent to the 3' region.

(Gerasimova et al., 2007)

P{EPgy2}CTCF^EY15833 is located 35bp downstream of the transcription start site of CTCF.

(Mohan et al., 2007)

Allele components

Component

Use(s)

Inserted element

P{EPgy2}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:7,356,662..7,356,662 [+]

(Gerasimova et al., 2007, Mohan et al., 2007, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

enhancer of variegation

(Macdonald et al., 2010)

fertile

(Macdonald et al., 2010, Gene Disruption Project members, 2001-)

homeotic

(Savitsky et al., 2016)

homeotic (with CTCF^y+1)

(Savitsky et al., 2016)

non-enhancer of variegation

(Macdonald et al., 2010)

viable

(Savitsky et al., 2016, Macdonald et al., 2010, Gene Disruption Project members, 2001-)

viable (with CTCF^y+1)

(Savitsky et al., 2016)

Phenotype Manifest In

abdominal segment 6

(Savitsky et al., 2016)

abdominal segment 6 (with CTCF^y+1)

(Savitsky et al., 2016)

abdominal segment 7

(Savitsky et al., 2016)

abdominal segment 7 (with CTCF^y+1)

(Savitsky et al., 2016)

abdominal segment 8

(Savitsky et al., 2016)

abdominal segment 8 (with CTCF^y+1)

(Savitsky et al., 2016)

abdominal sternite bristle

(Savitsky et al., 2016)

abdominal sternite bristle (with CTCF^y+1)

(Savitsky et al., 2016)

male genitalia (with CTCF^y+1)

(Savitsky et al., 2016)

sternite

(Savitsky et al., 2016)

sternite (with CTCF^y+1)

(Savitsky et al., 2016)

tergite

(Savitsky et al., 2016)

tergite (with CTCF^y+1)

(Savitsky et al., 2016)

Detailed Description

Statement

Reference

CTCF^EY15833/CTCF^EY15833 are viable, 10% of males and 20% of females have a wild type appearance, and show weak homeotic transformations (85% penetrance) of segments A7 and A6 towards A6 and A5: in males s6 (sternite of the sixth segment) has 0-4 bristles, t7 (tergite of the seventh segment) has 1 to several bristles (70%), or t7 is enlarged with a row of bristles (20%); in females s7 has 8-16 (average 13) bristles, occasionally 3 large bristles instead of 2, occasionally (<5%) s7 shape is transformed towards s6 and bristles lose orientation, and t8 has small bristles (20%).

CTCF^y+1/CTCF^EY15833 are viable and show homeotic transformations of A6-8: in males s6 has 0-6 (average 2.5) bristles, 15% have genitalia rotated by approximately 30[o], and t7 is well developed (100% penetrance); in females s7 has 9-14 (average 12) bristles, the shape of s7 is transformed toward s6 and bristles lose orientation (100%), and t8 has a row of small (100%) and several large (15%) bristles.

(Savitsky et al., 2016)

Homozygous adults appear healthy and are reasonably fertile.

Heterozygosity for CTCF^EY15833 substantially reduces the maternal, but not paternal, imprint of the g gene on the Dp(1;f)LJ9 mini-X chromosome.

(Macdonald et al., 2010)

CTCF^EY15833 mutants are viable and lack any visible phenotype.

(Gerasimova et al., 2007)

CTCF^EY15833 homozygotes and CTCF^EY15833/Df(3L)0463 appear as wild-type.

(Mohan et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

CTCF^EY15833/CTCF^y+1 has homeotic phenotype, enhanceable by mod(mdg4)^m9/mod(mdg4)^GS23019

(Savitsky et al., 2016)

CTCF^EY15833 has homeotic phenotype, enhanceable by mod(mdg4)^GS23019/mod(mdg4)^GS23019

(Savitsky et al., 2016)

NOT Enhanced by

Statement

Reference

CTCF^EY15833 has homeotic phenotype, non-enhanceable by mod(mdg4)^ul/mod(mdg4)^ul

(Savitsky et al., 2016)

Enhancer of

Statement

Reference

CTCF^EY15833/CTCF^EY15833 is an enhancer of homeotic phenotype of mod(mdg4)^GS23019

(Savitsky et al., 2016)

Other

Statement

Reference

CTCF^EY15833, mod(mdg4)^GS23019 has female semi-fertile phenotype

(Savitsky et al., 2016)

CTCF^EY15833/CTCF^y+1, mod(mdg4)^neo129/mod(mdg4)^m9 has lethal - all die during prepupal stage phenotype

(Savitsky et al., 2016)

CTCF^EY15833/CTCF^y+1, mod(mdg4)^m9/mod(mdg4)^GS23019 has lethal - all die before end of P-stage phenotype

(Savitsky et al., 2016)

CTCF^EY15833/CTCF^y+1, mod(mdg4)^m9/mod(mdg4)^GS23019 has majority die during prepupal stage phenotype

(Savitsky et al., 2016)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

CTCF^EY15833/CTCF^EY15833 mod(mdg4)^GS23019/mod(mdg4)^GS23019 double mutants are viable, males are fertile but females have reduced fertility and flies show stronger homeotic transformations of abdominal segments 6-8 than in either single mutant: in males, genitalia is rotated by 90-180[o] (100%), s6 has 4-15 (average 8) bristles, t7 is well developed, t8 is separated from t9 and bears 0-2 bristles; in females, s7 has 10-15 (average 13) bristles and t8 always has a row of bristles.

CTCF^EY15833/CTCF^EY15833 mod(mdg4)^ul/mod(mdg4)^ul double mutant phenotypes are the same as in single CTCF^EY15833/CTCF^EY15833 flies.

CTCF^y+1 mod(mdg4)^GS23019 / CTCF^EY15833 mod(mdg4)^m9 show early pupal lethality (stage P1-4), with rare escapers that die at pharate adult stage and show strong homeotic transformations (A6-8): in males genitalia is rotated by 180[o], s6 has 4-11 (average 9) bristles, display a small cuticle structure with 1-2 bristles interpreted as s7, t7 is well developed, and t8 is well separated from t9 and bears rows of large bristles; in females phenotypes are as in CTCF^y+1/CTCF^EY15833 but t8 always has rows of large bristles.

(Savitsky et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

21162

y¹ w^67c23; P{EPgy2}CTCF^EY15833

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

CTCF^EY15833

(Savitsky et al., 2016, Macdonald et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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