FB2024_03 , released June 25, 2024
Allele: Dmel\ThorΔ1034-2617
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General Information
Symbol
Dmel\ThorΔ1034-2617
Species
D. melanogaster
Name
FlyBase ID
FBal0190247
Feature type
allele
Associated gene
Dmel\Thor
Associated Insertion(s)
P{?lacW}ThorΔ1034-2617
Carried in Construct
Also Known As
d4E-BPnull, 4E-BPnull
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Tettweiler et al., 2005)
Mutagen
P-element activity
(Tettweiler et al., 2005)
Progenitor genotype
P{lacW}Thork13517
(Tettweiler et al., 2005)
Associated Insertion(s)
P{?lacW}ThorΔ1034-2617
Cytology
Description

Imprecise excision of the progenitor insertion (P{lacW}Thork13517) has resulted in a deletion that removes the entire Thor coding region. A 27bp fragment of the original P{lacW} element remains.

(Tettweiler et al., 2005)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
2L:3,478,437..3,480,015 [+]
Comment:

A 1579bp deletion resulting from the imprecise excision of P{lacW}Thork13517 removes the Thor coding region. 27 bases of the insertion (CATGATGAAATAACATATCAGATCATG) remain at the site of the excision. The deletion coordinates were determined from the reported junction sequences.

Inserted_sequence:

CATGATGAAATAACATATCAGATCATG

(Tettweiler et al., 2005)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      ThorΔ1034-2617 has no effect on the increase in sleep duration seen in wild type flies in response to rapamycin.

      (Metaxakis et al., 2014)

      The life-span of ThorΔ1034-2617 flies is significantly shorter in comparison to revertant ThorRv1 control flies. The median life-span of mutant males is 19.8 days, approximately 25% shorter than that of control males (26.6 days). The life-span of females is longer than in males, but a comparable relative effect of the ThorΔ1034-2617 mutation on life-span is observed in both sexes. ThorΔ1034-2617 larvae die substantially faster than their control counterparts under starvation (median life-span of 20.8 hours for starved ThorΔ1034-2617 larvae, 26.2 hours for wild-type). ThorΔ1034-2617 flies exhibit a reduced life-span when exposed to 5% hydrogen peroxide-containing media (median life-span of 23.2 hours). No ThorΔ1034-2617 flies survived 60 hours after exposure to oxidative stress, compared to 66.1% in wild-type.

      (Tettweiler et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      ThorΔ1034-2617 is a suppressor of viable | pupal stage phenotype of eIF4E107238/eIF-4E[+], parkP23

      (Ottone et al., 2011)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The pupal lethality observed in parkP23/parkP23 mutant animals is suppressed in a eIF-4E07238/+ background. Pupal lethality is restored in ThorΔ1034-2617/ThorΔ1034-2617; parkP23, eIF-4E07238/parkP23 animals.

      eIF-4E07238/+ significantly abrogates the oogenesis defects in parkP23/parkP23 mutant females. Oogenesis defects are not restored in ThorΔ1034-2617/ThorΔ1034-2617; parkP23, eIF-4E07238/parkP23 mutant animals.

      (Ottone et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      ThorΔ1034-2617 is rescued by ThorUAS.cTa/Scer\GAL4hs.PB

      (Tettweiler et al., 2005)
      Not rescued by

      ThorΔ1034-2617 is not rescued by ThorY54A.M59A.UAS/Scer\GAL4hs.PB

      (Tettweiler et al., 2005)
      Comments

      Expression of ThorScer\UAS.cTa under the control of Scer\GAL4hs.PB rescues the increased sensitivity of ThorΔ1034-2617 larvae to starvation. Expression of ThorY54A.M59A.Scer\UAS, under the control of Scer\GAL4hs.PB does not rescue the increased sensitivity of ThorΔ1034-2617 larvae to starvation. Expression of ThorScer\UAS.cTa under the control of Scer\GAL4hs.PB rescues oxidative stress sensitivity (median life-span of 55.4 hours). Expression of ThorY54A.M59A.Scer\UAS under the control of Scer\GAL4hs.PB does not rescue oxidative stress sensitivity (median life-span of 24.1 hours, 0.4% survival rate at 60 hours).

      (Tettweiler et al., 2005)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      4E-BPnull
      (Ottone et al., 2011, Tadros et al., 2007)
      4ebpnull
      (Hsieh et al., 2010)
      ThorΔ1034-2617
      d4E-BPNull
      (Malzer et al., 2018)
      d4E-BPnull
      (Omata et al., 2014, Tettweiler et al., 2005)
      Name Synonyms
      Secondary FlyBase IDs
        References (7)