FlyBase Allele Report: Dmel\Rab11[RNAi.UAS.WIZ]

General Information

Symbol

Dmel\Rab11^RNAi.UAS.WIZ

Species

D. melanogaster

Name

FlyBase ID

FBal0190337

Feature type

allele

Associated gene

Dmel\Rab11

Associated Insertion(s)

Carried in Construct

P{UAS-Rab11.RNAi.WIZ}

Also Known As

UAS-Rab11-RNAi, UAS-Rab11 RNAi, Rab11 RNAi

Key Links

Transgenic product class

RNAi_reagent

(Satoh et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Satoh et al., 2005)

Mutagen

in vitro construct

(Satoh et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Rab11.RNAi.WIZ}

Cytology

Description

UAS sequences drive expression of a pair of complementary 403bp fragments of Rab11, resulting in a construct which should produce Rab11 dsRNA.

(Satoh et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Rab11

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | adult stage | conditional, with Scer\GAL4^ninaE.PT

(Xiong et al., 2012)

increased cell death, with Scer\GAL4^GMR.PF

(Tiwari and Roy, 2009)

visible, with Scer\GAL4^GMR.PF

(Tiwari and Roy, 2009)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Tiwari and Roy, 2009)

eye photoreceptor cell, with Scer\GAL4^GMR.PF

(Houalla et al., 2010)

nephrocyte, with Scer\GAL4^Ugt36A1.PK, Scer\GAL80^ts.αTub84B

(Kampf et al., 2019)

rhabdomere, with Scer\GAL4^hs.PB

(Satoh et al., 2005)

slit diaphragm, with Scer\GAL4^Ugt36A1.PK, Scer\GAL80^ts.αTub84B

(Kampf et al., 2019)

wing hair | pupal stage, with Scer\GAL4^Act5C.PI

(Gault et al., 2012)

Detailed Description

Statement

Reference

Expression of Rab11^{dsRNA.UAS.WIZ} under the combined control of Scer\GAL4^Ugt36A1.PK and Gal80[ts] (for the temporal control of expression) leads to slit diagram localization defects (localized loss of slit diaphragms and shortened lines of slit diaphragm protein on the surface upon 2d expression; and extensive loss of slit diaphragm protein from the membrane upon 4d expression).

(Kampf et al., 2019)

Expression of Rab11^{dsRNA.Scer\UAS.WIZ} in somatic clones under the control of Scer\GAL4^Act5C.PI results in cells with short, missing or malformed trichomes in pupal wings.

(Gault et al., 2012)

Flies expressing Rab11^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^ninaE.PT which are raised at 18[o]C prior to eclosion and then raised under 12 hour light/12 hour dark conditions show a reduction in the amplitude of the electroretinogram at 3 weeks of age. This retinal degeneration defect is not seen if the flies are raised in constant darkness after eclosion.

(Xiong et al., 2012)

Expression of Rab11^{dsRNA.Scer\UAS.WIZ} in the eye, under the control of Scer\GAL4^GMR.PF results in R-cells failing to maintain their apical localization. Instead of forming a tightly associated R-cell cluster in the apical region, as observed in wild-type, R-cell clusters in Rab11^{dsRNA.Scer\UAS.WIZ} mutants extend in a long stretch with the distribution of R-cell nuclei along the entire apical-basal axis.

(Houalla et al., 2010)

Expression of Rab11^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^hs.PB results in small rhabdomeres.

(Satoh et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rab11^RNAi.UAS.WIZ, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by egr^UAS.cIa, Scer\GAL4^GMR.PF