FB2024_03 , released June 25, 2024
Allele: Dmel\PargUAS.EGFP
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General Information
Symbol
Dmel\PargUAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0191060
Feature type
allele
Associated gene
Dmel\Parg
Associated Insertion(s)
Carried in Construct
P{UAS-Parg.EGFP}
Also Known As
UAS::Parg-EGFP
Key Links
Transgenic product class
wild_type
(Tulin et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Tulin et al., 2006)
Mutagen
in vitro construct
(Tulin et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-Parg.EGFP}
Cytology
Description

UASt regulatory sequences drive expression of full-length Parg, which is tagged with EGFP.

(Tulin et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Parg
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies that express PargScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 are lethal; stage of lethality is dependent on insertion site and dosage of the transgene and driver. Cells expressing PargScer\UAS.T:Avic\GFP-EGFP, under the control of Scer\GAL4da.G32, show fragmented nucleoli. Third instar larvae expressing PargScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 fail to recover from a 40-min heat shock to the same extent as wild-type larvae (60% of mutants survive vs 99% of controls).

      (Tulin et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      PargUAS.EGFP, Scer\GAL4da.G32 has lethal phenotype, suppressible by Parp1I.UAS.DsRed(Unk), Scer\GAL4da.G32

      (Tulin et al., 2006)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The lethality that results from expression of PargScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 is rescued when ParpI.Scer\UAS.T:Disc\RFP is coexpressed.

      (Tulin et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      PargUAS.EGFP/Scer\GAL469B rescues Parg27.1

      (Kotova et al., 2009)

      PargUAS.EGFP/Scer\GAL469B rescues Parg27.1

      (Tulin et al., 2006)
      Comments

      Expression of PargScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL469B rescues the lethality of Parg27.1 animals.

      (Kotova et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      PargScer\UAS.T:Avic\GFP-EGFP
      PargUAS.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)