FlyBase Allele Report: Dmel\Adar[3.4.UAS]

General Information

Symbol

Dmel\Adar^3.4.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0191441

Feature type

allele

Associated gene

Dmel\Adar

Associated Insertion(s)

Carried in Construct

P{UAS-Adar.3.4}

Also Known As

UAS-Adar

Key Links

Transgenic product class

cDNA

(Keegan et al., 2005)

wild_type

(Keegan et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Keegan et al., 2005)

wild_type

(Keegan et al., 2005)

Mutagen

in vitro construct

(Keegan et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Adar.3.4}

Cytology

Description

UASt regulatory sequences drive expression of cDNA encoding the Adar 3/4 isoform. The coding region is tagged with both Tag:FLAG and Tag:polyHis.

(Keegan et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight | adult stage, with Scer\GAL4^elav-C155

(Varte et al., 2022)

lethal, with Scer\GAL4^Act5C.PU

(Deng et al., 2020)

partially lethal - majority die | male, with Adar^1F4, Scer\GAL4^Act5C.PI

(Keegan et al., 2005)

viable, with Scer\GAL4^Mef2.PR

(Keegan et al., 2005)

Phenotype Manifest In

Detailed Description

Statement

Reference

Among male progeny of rescue crosses that carry both the Adar^1F4 mutation and the Adar^3.4.Scer\UAS construct, the number of Scer\GAL4^Act5C.PI driver flies obtained, expressed as a percentage of the number of control flies lacking a GAL4 driver, is 20%.

(Keegan et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Adar^3.4.UAS, Scer\GAL4^elav-C155 is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^elav-C155, tx^UAS.cMa

(Varte et al., 2022)

NOT Suppressor of

Statement

Reference

Adar^3.4.UAS, Scer\GAL4^elav-C155 is a non-suppressor of abnormal flight | adult stage phenotype of Scer\GAL4^elav-C155, tx^UAS.cMa

(Varte et al., 2022)

Phenotype Manifest In

Suppressor of

Statement

Reference

Adar^3.4.UAS, Scer\GAL4^elav-C155 is a suppressor | partially of wing phenotype of Scer\GAL4^elav-C155, tx^UAS.cMa

(Varte et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^βtub.PU/Adar^3.4.UAS rescues Adar^5G1

(Bhogal et al., 2011)

Scer\GAL4^elav-C155/Adar^3.4.UAS rescues Adar^5G1

(Bhogal et al., 2011)

Scer\GAL4^scrt-11-6/Adar^3.4.UAS rescues Adar^5G1

(Bhogal et al., 2011)

Scer\GAL4^ChAT.7.4/Adar^3.4.UAS rescues Adar^5G1

(Keegan et al., 2011)

Scer\GAL4^ChAT.7.4/Adar^3.4.UAS rescues Adar^1F4

(Keegan et al., 2011)

Partially rescues

Scer\GAL4^ChAT.7.4/Adar^3.4.UAS partially rescues Adar^5G1

(Deng et al., 2020)

Scer\GAL4^ChAT.7.4/Adar^3.4.UAS partially rescues Adar^E374A

(Deng et al., 2020)

Scer\GAL4^Act5C.PI/Adar^3.4.UAS partially rescues Adar^1F4

(Keegan et al., 2005)

Fails to rescue

Scer\GAL4^Mhc.PU/Adar^3.4.UAS fails to rescue Adar^5G1

(Bhogal et al., 2011)

Scer\GAL4^G14/Adar^3.4.UAS fails to rescue Adar^5G1

(Bhogal et al., 2011)

Comments

Ubiquitous expression of Adar^3.4.Scer\UAS under the control of Scer\GAL4^βtub.PU completely rescues the neuromuscular junction phenotype observed in Adar^5G1 larvae with respect to synaptic bouton number and branching.

(Bhogal et al., 2011)

Expression of Adar^3.4.Scer\UAS under the control of Scer\GAL4^Cha.7.4 rescues the locomotor defects seen in Adar^5G1 and in Adar^1F4 adults.

Expression of Adar^3.4.Scer\UAS under the control of Scer\GAL4^Cha.7.4 significantly rescues the neurodegeneration phenotype seen in the mushroom body calyces and retina of 30 day old Adar^5G1 adults.

(Keegan et al., 2011)

Expression of Adar^3.4.Scer\UAS, under the control of Scer\GAL4^Act5C.PI, in a Adar^1F4 background partially rescues the Adar^1F4 mutant phenotype.

(Keegan et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Adar^3.4.Scer\UAS

Adar^3.4.UAS

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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