FlyBase Allele Report: Dmel\Met[W3]

General Information

Symbol

Dmel\Met^W3

Species

D. melanogaster

Name

FlyBase ID

FBal0191692

Feature type

allele

Associated gene

Dmel\Met

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

gamma ray

Nature of the Allele

Allele class

Mutagen

gamma ray

(Wilson et al., 2006)

Progenitor genotype

Cytology

Description

Amino acid replacement: E5G.

(Wilson et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:11,616,881..11,616,881 [+]

Nucleotide change:

A11616881G

Amino acid change:

E5G | Met-PA; E5G | Met-PB

Reported amino acid change:

E5G

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Wilson et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical resistant

(Wilson et al., 2006)

visible

(Baumann et al., 2010, Wilson et al., 2006)

Phenotype Manifest In

embryonic/larval fat body | wandering third instar larval stage, with Met^UAS.cBa, Scer\GAL4^Adh.PF

(Liu et al., 2009)

eye

(Wilson et al., 2006)

ommatidium

(Baumann et al., 2010)

Detailed Description

Statement

Reference

Mutants have defective facets in the posterior part of the eye.

(Baumann et al., 2010)

In addition to delayed larval development, the body weight of animals expressing Met^Scer\UAS.cBa driven by Scer\GAL4^Adh.PF in a hemizygous Met^W3 mutant background is dramatically reduced in comparison to Scer\GAL4^Adh.PF controls. Once these mutants reach wandering third instar larval stage, their fat body cells begin to dissociate and undergo dramatic apoptosis.

(Liu et al., 2009)

Homozygotes show increased resistance to methoprene compared to controls.

Homozygous females have a reduced rate of oviposition compared to controls.

78% of mutant flies have a small number of defective ommatidia in the posterior quarter of the eye.

(Wilson et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Met^W3 has visible phenotype, enhanceable by gce^RNAi.UAS.cBa/Scer\GAL4^Act5C.PI

(Baumann et al., 2010)

Met^W3 has visible phenotype, enhanceable by br^rbp-2

(Wilson et al., 2006)

Suppressed by

Statement

Reference

Met^W3 has visible phenotype, suppressible by gce^UAS.cBa/Scer\GAL4^Tub.PU

(Baumann et al., 2010)

Met^W3 has visible phenotype, suppressible by gce^UAS.cBa/Scer\GAL4^dan-AC116

(Baumann et al., 2010)

NOT suppressed by

Statement

Reference

Met^W3 has visible phenotype, non-suppressible by gce^UAS.cBa/Scer\GAL4^Lsp2.PH

(Baumann et al., 2010)

Other

Statement

Reference

Met^W3, br¹ has lethal - all die during P-stage phenotype

(Wilson et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Met^W3 has ommatidium phenotype, enhanceable by gce^RNAi.UAS.cBa/Scer\GAL4^Act5C.PI

(Baumann et al., 2010)

Met^W3 has eye phenotype, enhanceable by br^rbp-2

(Wilson et al., 2006)

Suppressed by

Statement

Reference

Met^W3 has ommatidium phenotype, suppressible by gce^UAS.cBa/Scer\GAL4^Tub.PU

(Baumann et al., 2010)

Met^W3 has ommatidium phenotype, suppressible by gce^UAS.cBa/Scer\GAL4^dan-AC116

(Baumann et al., 2010)

NOT suppressed by

Statement

Reference

Met^W3 has ommatidium phenotype, non-suppressible by gce^UAS.cBa/Scer\GAL4^Lsp2.PH

(Baumann et al., 2010)

Enhancer of

Statement

Reference

Met^W3/Met^W3 is an enhancer of ovary phenotype of br¹

(Wilson et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The defective facet phenotype seen in Met^W3 flies is suppressed by expression of gce^Scer\UAS.cBa under the control of either Scer\GAL4^tub.PU or Scer\GAL4^dan-AC116, but is not suppressed by expression of gce^Scer\UAS.cBa under the control of Scer\GAL4^Lsp2.PH.

Expression of gce^{dsRNA.Scer\UAS.cBa} under the control of Scer\GAL4^Act5C.PI enhances the defective facet phenotype seen in Met^W3 flies.

(Baumann et al., 2010)

br¹, Rst(1)JH^W3 double mutant escapers show significantly reduced levels of oogenesis compared to br¹ single mutants.

(Wilson et al., 2006)

The expressivity of the defective eye phenotype seen in Met^W3 flies is increased by br^rbp-2 but not by br¹.

(Wilson et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Met^W3 is rescued by Met^UAS.cBa

(Barry et al., 2008)

Met^W3 is rescued by Scer\GAL4^Act.PU/Met^UAS.cBa

(Barry et al., 2008)

Comments

Either of the transgenic lines Met^hs.PA or P{UAS-Met.B}pV2 can rescue the Met^W3 mutant eye phenotype due to their Scer\GAL4-independent expression of Met^Scer\UAS.cBa. The methoprene resistance of Met^W3 mutants is also lost as a result of Met^Scer\UAS.cBa expression.

Scer\GAL4^Act-driven Met^Scer\UAS.cBa rescues the Met^W3 mutant eye phenotype.

(Barry et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

90980

w^* v^* Met^W3; Kr^If-1/SM5

90981

w^* v^* Met^W3 gce^2.5k/FM7c, P{2xTb¹-RFP}FM7c

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Met^W3

(Baumann et al., 2010, Barry et al., 2008, Wilson et al., 2006, Wilson et al., 2006)

Met^w3

(Liu et al., 2009)

Rst(1)JH^W3

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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