FB2024_03 , released June 25, 2024
Allele: Dmel\traF.U2af50
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General Information
Symbol
Dmel\traF.U2af50
Species
D. melanogaster
Name
FlyBase ID
FBal0193397
Feature type
allele
Associated gene
Dmel\tra
Associated Insertion(s)
Carried in Construct
P{U2af50-traF}
Also Known As
U2af-traF
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Evans and Cline, 2007)
Progenitor genotype
Carried in construct
P{U2af50-traF}
Cytology
Description

Contains 4.5kb of U2af50 upstream genomic sequence, followed by the 216bp U2af50 5' UTR with an improved Cavener translation initiation sequence, followed by the tripeptide MAS and the full female-specific tra open reading frame, followed by the 77bp U2af50 3' UTR and adjacent 1.5kb downstream genomic sequence.

(Evans and Cline, 2007)
Allele components
Component
Use(s)
Regulatory region(s)
U2af50
Encoded product / tool
tra
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The XY germline degenerates in XY animals expressing traF.U2af50.

      (Yang et al., 2012)

      In mosaic XX/XO animals (gynanders) which are carrying traF.U2af50, where all the somatic cells of both gonads (those cells inside of the epithelial sheath that surrounds each ovariole) are XO, then the XO somatic cells support normal oogenesis of XX germ cells, resulting in morphologically wild-type eggs. 33% of these gynanders lay their eggs and all those that lay eggs are fertile.

      In mosaic XX/XO animals (gynanders) which are carrying traF.U2af50, XO germ cells form tumorous egg chambers, both when the somatic cells of the gonad (those cells inside of the epithelial sheath that surrounds each ovariole) are XO and also when the somatic cells of the gonad are XX.

      (Evans and Cline, 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      traF.U2af50 feminizes Sxlf7,M1/SxlM1,fΔ33 mutants without increasing their size. Sxlf7,M1/SxlM1,fΔ33 ; traF.U2af50/+ females have large ovaries full of mature eggs, but they fail to lay.

      (Evans and Cline, 2013)

      Phf7ΔN2 significantly rescues germ cell number and organisation in XY animals expressing traF.U2af50.

      (Yang et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      traF.U2af50 rescues tra1/Df(3L)st-j7

      (Evans and Cline, 2007)

      traF.U2af50 rescues tra[-]

      (Evans and Cline, 2007)
      Comments

      XX tra1/Df(3L)st-j7 individuals are feminised by traF.U2af50 and the rescued females are fertile.

      XX tra- individuals derived from a cross of tra1/Df(3L)st-j7 females to tra1/TM6 males are feminised by traF.U2af50 and the rescued females are fertile.

      XX tra- individuals derived from a cross of tra1/Df(3L)st-j7 females to tra1/TM6 males are feminised by traF.U2af50 and the rescued females are fertile.

      XX tra- individuals derived from a cross of tra1/Df(3L)st-j7 females to tra5/TM6 males are feminised by traF.U2af50 and the rescued females are fertile.

      (Evans and Cline, 2007)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      traF.U2af50
      Name Synonyms
      Secondary FlyBase IDs
        References (4)