FlyBase Allele Report: Dmel\CadN[Δ14]

General Information

Symbol

Dmel\CadN^Δ14

Species

D. melanogaster

Name

FlyBase ID

FBal0193539

Feature type

allele

Associated gene

Dmel\CadN

Associated Insertion(s)

Carried in Construct

Also Known As

ncad^Δ14

Key Links

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

(Prakash et al., 2005)

Progenitor genotype

P{GT1}BG01221

(Prakash et al., 2005)

Caused by aberration

Df(2L)CadN^Δ14

Cytology

Description

X-ray-mediated deletion of P{GT1}BG01221, resulting in deletion of the entire CadN2 gene, part of the CadN gene and sequences in between the two genes (including the P{GT1}BG01221 element).

(Prakash et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Nern et al., 2008, Choe et al., 2006)

abnormal neuroanatomy | adult stage | somatic clone

(Pecot et al., 2013)

abnormal neuroanatomy | P-stage | somatic clone

(Pecot et al., 2013)

abnormal neuroanatomy | pupal stage | somatic clone

(Prakash et al., 2009)

abnormal neuroanatomy | somatic clone

(Tong et al., 2011)

Phenotype Manifest In

eye photoreceptor cell

(Choe et al., 2006)

inner medulla | adult stage | somatic clone

(Pecot et al., 2013)

inner medulla | P-stage | somatic clone

(Pecot et al., 2013)

lamina

(Choe et al., 2006)

lamina monopolar neuron

(Nern et al., 2008)

lamina monopolar neuron L1 | adult stage | somatic clone

(Pecot et al., 2013)

lamina monopolar neuron L1 | P-stage | somatic clone

(Pecot et al., 2013)

lamina monopolar neuron L2 | adult stage | somatic clone

(Pecot et al., 2013)

lamina monopolar neuron L3 | adult stage | somatic clone

(Pecot et al., 2013)

lamina monopolar neuron L3 | P-stage | somatic clone

(Pecot et al., 2013)

lamina monopolar neuron L4 | adult stage | somatic clone

(Pecot et al., 2013)

lamina monopolar neuron L5 | P-stage | somatic clone

(Pecot et al., 2013)

medulla

(Choe et al., 2006)

medulla layer M6 | adult stage | somatic clone

(Pecot et al., 2013)

medulla layer M6 | P-stage | somatic clone

(Pecot et al., 2013)

optic cartridge

(Choe et al., 2006)

outer medulla | adult stage | somatic clone

(Pecot et al., 2013)

photoreceptor cell R4 | cell non-autonomous | somatic clone

(Choe et al., 2006)

photoreceptor cell R4 | pupal stage | somatic clone

(Prakash et al., 2009)

photoreceptor cell R7

(Nern et al., 2008)

photoreceptor cell R7 | somatic clone

(Tong et al., 2011)

photoreceptor neuron | pupal stage | somatic clone

(Prakash et al., 2009)

Detailed Description

Statement

Reference

chp staining of the medulla of CadN^Δ14 eye clones (made using the 'eyFLP' system) reveals a few 'gaps' as if some R7 terminals are missing.

CadN^Δ14 eye clones (made using the 'eyFLP' system) have R7 targeting phenotypes.

(Tong et al., 2011)

When large clones of CadN^Δ14 mutant cells are generated in the eye, R4 axons frequently fail to extend, or extend aberrantly.

(Prakash et al., 2009)

Using MARCM, mistargeting of CadN^Δ14 mutant L1-L5 cells is seen at the following percentages: 29% of L1 neurons, typically terminating in M10 rather than M5; 0% of L2 neurons; 81% of L3 neuron, typically terminating in M5 and M6 rather than M3; 81% of L4 neurons, terminating in M2 or, less frequently, M8 instead of M4; 0% of L5 terminals, but 100% of L5 interstitial branches fail to extend from M1 into M2. 100% of CadN^Δ14 mutant R7 cells mistarget. 4% of R8 cells extend significantly deeper into the medulla than the remaining R8 cells, similar to controls.

At 25hrs APF, the centres of CadN^Δ14 L3 growth cones are located in the R7 temporary layer about 2υm more proximal than in wild-type L3s. Mutant L3 terminals continue to exhibit incorrect layer choice, and remain elongated along one side of the column with only some diffuse lateral processes.

When using a reverse MARCM approach, 44/825 wildtype L5 neurons, neighboured by mutant cells, are observed in which almost no interstitial branches extend into M2 but instead send branches into adjacent columns, compared to controls which show no targeting defects.

(Nern et al., 2008)

CadN^Δ14 mutants exhibit a specific pattern of disruption in the structure of the cartridge, the synaptic unit in the lamina. Some cartridges have either >6 or <6 R cells axons, and some adjacent cartridges fuse.

In CadN^Δ14 mutants, R7 axons frequently stop at abnormally distal positions within the R8 recipient layer, leaving gaps in the array of otherwise regular R7 termini. However the ganglion-specific targeting of R1-R6 axons to the lamina nor the layer-specific targeting of R8 axons within the medulla are affected.

In CadN^Δ14 homozygous mutants, the pattern of cartridges (where the R cell axons and lamina neuron processes form a highly organised fascicle) is severely disrupted.

In CadN^Δ14 mutant target clones, the array of R4 axons appear disrupted, with many axons failing to extend, while others target inappropriately.

(Choe et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

CadN^Δ14 has abnormal neuroanatomy | adult stage | somatic clone phenotype, enhanceable by Sema1a^k13702/Sema1a^k13702

(Pecot et al., 2013)

CadN^Δ14 has abnormal neuroanatomy | P-stage | somatic clone phenotype, enhanceable by Sema1a^k13702/Sema1a^k13702

(Pecot et al., 2013)

NOT suppressed by

Statement

Reference

CadN^Δ14 has abnormal neuroanatomy | somatic clone | pupal stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Lar^UAS.cKa

(Prakash et al., 2009)

Enhancer of

Statement

Reference

CadN^Δ14/CadN^Δ14 is an enhancer of abnormal neuroanatomy | adult stage | somatic clone phenotype of Sema1a^k13702

(Pecot et al., 2013)

CadN^Δ14/CadN^Δ14 is an enhancer of abnormal neuroanatomy | P-stage | somatic clone phenotype of Sema1a^k13702

(Pecot et al., 2013)

CadN[+]/CadN^Δ14 is an enhancer of lethal phenotype of Lar²¹²⁷/Lar⁴⁵¹

(Prakash et al., 2009)

NOT Enhancer of

Statement

Reference

CadN[+]/CadN^Δ14 is a non-enhancer of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Mirkovic et al., 2011)

Suppressor of

Statement

Reference

CadN[+]/CadN^Δ14 is a suppressor | partially of lethal phenotype of Liprin-α^E/Liprin-α¹

(Prakash et al., 2009)

CadN[+]/CadN^Δ14 is a suppressor | partially of lethal phenotype of Liprin-α^F/Liprin-α^E

(Prakash et al., 2009)

NOT Suppressor of

Statement

Reference

CadN[+]/CadN^Δ14 is a non-suppressor of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Mirkovic et al., 2011)

Other

Statement

Reference

CadN^Δ14, Rich¹ has abnormal neuroanatomy | somatic clone phenotype

(Tong et al., 2011)

CadN[+]/CadN^Δ14, Lar²¹²⁷/Lar[+], Liprin-α^F has partially lethal - majority die | dominant phenotype

(Prakash et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

CadN^Δ14 has lamina monopolar neuron L2 | adult stage | somatic clone phenotype, enhanceable by Sema1a^k13702/Sema1a^k13702

(Pecot et al., 2013)

CadN^Δ14 has lamina monopolar neuron L4 | adult stage | somatic clone phenotype, enhanceable by Sema1a^k13702/Sema1a^k13702

(Pecot et al., 2013)

CadN^Δ14 has lamina monopolar neuron L3 | adult stage | somatic clone phenotype, enhanceable by Sema1a^k13702/Sema1a^k13702

(Pecot et al., 2013)

CadN^Δ14 has lamina monopolar neuron L3 | P-stage | somatic clone phenotype, enhanceable by Sema1a^k13702/Sema1a^k13702

(Pecot et al., 2013)

CadN^Δ14 has lamina monopolar neuron L1 | P-stage | somatic clone phenotype, enhanceable by Sema1a^k13702/Sema1a^k13702

(Pecot et al., 2013)

CadN^Δ14 has lamina monopolar neuron L1 | adult stage | somatic clone phenotype, enhanceable by Sema1a^k13702/Sema1a^k13702

(Pecot et al., 2013)

CadN^Δ14 has lamina monopolar neuron L5 | P-stage | somatic clone phenotype, enhanceable by Sema1a^k13702/Sema1a^k13702

(Pecot et al., 2013)

NOT suppressed by

Statement

Reference

CadN^Δ14 has photoreceptor neuron | somatic clone | pupal stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Lar^UAS.cKa

(Prakash et al., 2009)

Enhancer of

Statement

Reference

CadN^Δ14/CadN^Δ14 is an enhancer of lamina monopolar neuron L3 | adult stage | somatic clone phenotype of Sema1a^k13702

(Pecot et al., 2013)

CadN^Δ14/CadN^Δ14 is an enhancer of lamina monopolar neuron L1 | P-stage | somatic clone phenotype of Sema1a^k13702

(Pecot et al., 2013)

CadN^Δ14/CadN^Δ14 is an enhancer of lamina monopolar neuron L1 | adult stage | somatic clone phenotype of Sema1a^k13702

(Pecot et al., 2013)

CadN^Δ14/CadN^Δ14 is an enhancer of lamina monopolar neuron L5 | P-stage | somatic clone phenotype of Sema1a^k13702

(Pecot et al., 2013)

CadN^Δ14/CadN^Δ14 is an enhancer of lamina monopolar neuron L5 | adult stage | somatic clone phenotype of Sema1a^k13702

(Pecot et al., 2013)

NOT Enhancer of

Statement

Reference

CadN[+]/CadN^Δ14 is a non-enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Mirkovic et al., 2011)

NOT Suppressor of

Statement

Reference

CadN[+]/CadN^Δ14 is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Mirkovic et al., 2011)

CadN^Δ14 is a non-suppressor of ommatidium phenotype of ec^spok

(Montrasio et al., 2007)

Other

Statement

Reference

CadN^Δ14, Rich¹ has photoreceptor cell R7 | somatic clone phenotype

(Tong et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

CadN^Δ14, Rich¹ double mutant eye clones (made using the 'eyFLP' system) have R7 targeting phenotypes similar to CadN^Δ14 single mutants.

(Tong et al., 2011)

When large clones of cells mutant for both Liprin-α^E and CadN^Δ14 are generated in the eye, R4 axon targeting errors are more frequently observed than in single mutants.

When large clones of cells mutant for both Lar²¹²⁷ and CadN^Δ14 are generated in the eye, R4 axon targeting errors are more frequently observed than in single mutants.

Expression of Lar^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 in CadN^Δ14 somatic mutant clones does not rescue the photoreceptor targeting defects.

When large clones of cells mutant for Liprin-α^E, Lar²¹²⁷, and CadN^Δ14 are generated in the eye, R4 axon targeting errors are observed, but the frequency of defects is similar to Liprin-α^E; Lar²¹²⁷, Lar²¹²⁷; CadN^Δ14, or Liprin-α^E; CadN^Δ14 double mutant combinations.

(Prakash et al., 2009)

The ommatidial rotation defects observed in ec^spok hemizygous adult eyes are not suppressed by CadN^Δ14/+.

(Montrasio et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CadN^Δ14

(Fic et al., 2021, Tong et al., 2011, Nern et al., 2008)

CadNΔ14

(Pecot et al., 2013)

N-cadherin^Δ14

(Prakash et al., 2009)

Ncad^Δ14

(Schwabe et al., 2013, Mirkovic et al., 2011, Montrasio et al., 2007)

ncad^Δ14

(Gontang et al., 2011)

Δ14

(Prakash et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

Open Close